Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0871 and Shewana3_0872 are separated by 41 nucleotides Shewana3_0872 and Shewana3_0873 are separated by 264 nucleotides Shewana3_0873 and Shewana3_0874 are separated by 123 nucleotides
Shewana3_0871: Shewana3_0871 - protoporphyrinogen oxidase (RefSeq), at 1,017,066 to 1,017,587
_0871
Shewana3_0872: Shewana3_0872 - hypothetical protein (RefSeq), at 1,017,629 to 1,018,156
_0872
Shewana3_0873: Shewana3_0873 - putative lipoprotein (RefSeq), at 1,018,421 to 1,019,428
_0873
Shewana3_0874: Shewana3_0874 - DSBA oxidoreductase (RefSeq), at 1,019,552 to 1,020,304
_0874
Position (kb)
1018
1019
1020 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1017.714 kb on + strand, within Shewana3_0872 at 1017.746 kb on - strand, within Shewana3_0872 at 1017.746 kb on - strand, within Shewana3_0872 at 1017.799 kb on - strand, within Shewana3_0872 at 1017.916 kb on + strand, within Shewana3_0872 at 1017.916 kb on + strand, within Shewana3_0872 at 1017.955 kb on + strand, within Shewana3_0872 at 1017.968 kb on - strand, within Shewana3_0872 at 1018.001 kb on + strand, within Shewana3_0872 at 1018.290 kb on + strand at 1018.298 kb on - strand at 1018.335 kb on + strand at 1018.490 kb on - strand at 1018.702 kb on + strand, within Shewana3_0873 at 1019.004 kb on + strand, within Shewana3_0873 at 1019.012 kb on - strand, within Shewana3_0873 at 1019.396 kb on + strand at 1019.429 kb on + strand at 1019.872 kb on + strand, within Shewana3_0874 at 1020.039 kb on + strand, within Shewana3_0874 at 1020.093 kb on + strand, within Shewana3_0874 at 1020.100 kb on + strand, within Shewana3_0874 at 1020.186 kb on - strand, within Shewana3_0874 at 1020.295 kb on - strand at 1020.295 kb on - strand at 1020.310 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,017,714 + Shewana3_0872 0.16 -1.4 1,017,746 - Shewana3_0872 0.22 +0.9 1,017,746 - Shewana3_0872 0.22 +0.9 1,017,799 - Shewana3_0872 0.32 +0.7 1,017,916 + Shewana3_0872 0.54 +0.6 1,017,916 + Shewana3_0872 0.54 -2.4 1,017,955 + Shewana3_0872 0.62 +0.8 1,017,968 - Shewana3_0872 0.64 -1.3 1,018,001 + Shewana3_0872 0.70 -3.1 1,018,290 + +0.7 1,018,298 - +0.5 1,018,335 + -1.5 1,018,490 - +0.8 1,018,702 + Shewana3_0873 0.28 +0.5 1,019,004 + Shewana3_0873 0.58 +0.6 1,019,012 - Shewana3_0873 0.59 +0.9 1,019,396 + -0.0 1,019,429 + +0.5 1,019,872 + Shewana3_0874 0.42 +0.3 1,020,039 + Shewana3_0874 0.65 -1.4 1,020,093 + Shewana3_0874 0.72 +0.6 1,020,100 + Shewana3_0874 0.73 -1.7 1,020,186 - Shewana3_0874 0.84 +0.1 1,020,295 - -0.0 1,020,295 - -1.3 1,020,310 + +0.8
Or see this region's nucleotide sequence