Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0857 and Shewana3_0858 are separated by 676 nucleotides Shewana3_0858 and Shewana3_0859 are separated by 367 nucleotides
Shewana3_0857: Shewana3_0857 - phosphoadenosine phosphosulfate reductase (RefSeq), at 996,944 to 997,741
_0857
Shewana3_0858: Shewana3_0858 - monofunctional biosynthetic peptidoglycan transglycosylase (RefSeq), at 998,418 to 999,203
_0858
Shewana3_0859: Shewana3_0859 - hypothetical protein (RefSeq), at 999,571 to 1,001,307
_0859
Position (kb) 998
999
1000 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 997.571 kb on + strand, within Shewana3_0857 at 998.035 kb on + strand at 998.035 kb on + strand at 998.176 kb on + strand at 998.219 kb on - strand at 998.239 kb on - strand at 998.259 kb on - strand at 998.334 kb on + strand at 998.690 kb on + strand, within Shewana3_0858 at 998.762 kb on - strand, within Shewana3_0858 at 998.818 kb on - strand, within Shewana3_0858 at 998.827 kb on + strand, within Shewana3_0858 at 998.883 kb on - strand, within Shewana3_0858 at 998.883 kb on - strand, within Shewana3_0858 at 998.924 kb on + strand, within Shewana3_0858 at 998.944 kb on + strand, within Shewana3_0858 at 999.072 kb on + strand, within Shewana3_0858 at 999.077 kb on + strand, within Shewana3_0858 at 999.083 kb on + strand at 999.107 kb on + strand, within Shewana3_0858 at 999.107 kb on + strand, within Shewana3_0858 at 999.118 kb on + strand, within Shewana3_0858 at 999.121 kb on - strand, within Shewana3_0858 at 999.121 kb on - strand, within Shewana3_0858 at 999.126 kb on - strand at 999.126 kb on - strand at 999.143 kb on - strand at 999.163 kb on + strand at 999.175 kb on + strand at 999.346 kb on - strand at 999.395 kb on - strand at 999.417 kb on + strand at 999.462 kb on + strand at 999.462 kb on + strand at 999.462 kb on + strand at 999.470 kb on - strand at 999.470 kb on - strand at 999.597 kb on + strand at 999.641 kb on + strand at 999.649 kb on - strand at 999.722 kb on - strand at 999.849 kb on + strand, within Shewana3_0859 at 999.849 kb on + strand, within Shewana3_0859 at 999.849 kb on + strand, within Shewana3_0859 at 999.849 kb on + strand, within Shewana3_0859 at 999.849 kb on + strand, within Shewana3_0859 at 999.849 kb on + strand, within Shewana3_0859 at 999.849 kb on + strand, within Shewana3_0859 at 999.849 kb on + strand, within Shewana3_0859 at 999.849 kb on + strand, within Shewana3_0859 at 999.857 kb on - strand, within Shewana3_0859 at 999.857 kb on - strand, within Shewana3_0859 at 999.857 kb on - strand, within Shewana3_0859 at 999.882 kb on + strand, within Shewana3_0859 at 999.891 kb on - strand, within Shewana3_0859 at 1000.127 kb on + strand, within Shewana3_0859
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 997,571 + Shewana3_0857 0.79 -5.3 998,035 + +0.5 998,035 + +0.2 998,176 + +0.9 998,219 - +0.9 998,239 - -1.3 998,259 - -1.1 998,334 + -2.0 998,690 + Shewana3_0858 0.35 -1.5 998,762 - Shewana3_0858 0.44 +0.8 998,818 - Shewana3_0858 0.51 +1.1 998,827 + Shewana3_0858 0.52 +0.5 998,883 - Shewana3_0858 0.59 +0.8 998,883 - Shewana3_0858 0.59 +0.4 998,924 + Shewana3_0858 0.64 -0.8 998,944 + Shewana3_0858 0.67 +1.3 999,072 + Shewana3_0858 0.83 +0.4 999,077 + Shewana3_0858 0.84 +0.3 999,083 + +0.4 999,107 + Shewana3_0858 0.88 +0.4 999,107 + Shewana3_0858 0.88 -1.8 999,118 + Shewana3_0858 0.89 +0.3 999,121 - Shewana3_0858 0.89 +0.1 999,121 - Shewana3_0858 0.89 +0.7 999,126 - -1.9 999,126 - -1.6 999,143 - +0.1 999,163 + +0.7 999,175 + +0.7 999,346 - +0.6 999,395 - +0.5 999,417 + -0.3 999,462 + -1.3 999,462 + -2.3 999,462 + +0.5 999,470 - +0.6 999,470 - -1.9 999,597 + +0.7 999,641 + -0.6 999,649 - +0.6 999,722 - +0.1 999,849 + Shewana3_0859 0.16 +0.6 999,849 + Shewana3_0859 0.16 -1.1 999,849 + Shewana3_0859 0.16 -1.6 999,849 + Shewana3_0859 0.16 -1.4 999,849 + Shewana3_0859 0.16 +0.5 999,849 + Shewana3_0859 0.16 -1.1 999,849 + Shewana3_0859 0.16 +3.3 999,849 + Shewana3_0859 0.16 +0.8 999,849 + Shewana3_0859 0.16 -1.6 999,857 - Shewana3_0859 0.16 +1.0 999,857 - Shewana3_0859 0.16 +0.7 999,857 - Shewana3_0859 0.16 -0.0 999,882 + Shewana3_0859 0.18 +1.2 999,891 - Shewana3_0859 0.18 -1.1 1,000,127 + Shewana3_0859 0.32 +0.5
Or see this region's nucleotide sequence