Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0833 and Shewana3_0834 are separated by 98 nucleotides Shewana3_0834 and Shewana3_0835 are separated by 130 nucleotides
Shewana3_0833: Shewana3_0833 - diguanylate cyclase with GAF sensor (RefSeq), at 965,846 to 966,814
_0833
Shewana3_0834: Shewana3_0834 - hypothetical protein (RefSeq), at 966,913 to 967,872
_0834
Shewana3_0835: Shewana3_0835 - LysR family transcriptional regulator (RefSeq), at 968,003 to 968,986
_0835
Position (kb) 966
967
968 Strain fitness (log2 ratio) -2
-1
0
1 at 966.018 kb on + strand, within Shewana3_0833 at 966.490 kb on + strand, within Shewana3_0833 at 966.490 kb on + strand, within Shewana3_0833 at 966.498 kb on - strand, within Shewana3_0833 at 966.498 kb on - strand, within Shewana3_0833 at 966.498 kb on - strand, within Shewana3_0833 at 966.498 kb on - strand, within Shewana3_0833 at 966.558 kb on - strand, within Shewana3_0833 at 966.625 kb on - strand, within Shewana3_0833 at 966.810 kb on - strand at 966.816 kb on - strand at 966.862 kb on + strand at 966.932 kb on + strand at 966.983 kb on + strand at 967.064 kb on - strand, within Shewana3_0834 at 967.289 kb on + strand, within Shewana3_0834 at 967.297 kb on - strand, within Shewana3_0834 at 967.381 kb on + strand, within Shewana3_0834 at 967.389 kb on - strand, within Shewana3_0834 at 967.389 kb on - strand, within Shewana3_0834 at 967.393 kb on + strand, within Shewana3_0834 at 967.480 kb on - strand, within Shewana3_0834 at 967.634 kb on - strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.654 kb on - strand, within Shewana3_0834 at 967.749 kb on + strand, within Shewana3_0834 at 967.757 kb on - strand, within Shewana3_0834 at 967.781 kb on + strand at 968.067 kb on + strand at 968.196 kb on - strand, within Shewana3_0835 at 968.237 kb on - strand, within Shewana3_0835 at 968.258 kb on + strand, within Shewana3_0835 at 968.313 kb on + strand, within Shewana3_0835 at 968.401 kb on + strand, within Shewana3_0835 at 968.409 kb on - strand, within Shewana3_0835 at 968.578 kb on - strand, within Shewana3_0835 at 968.744 kb on + strand, within Shewana3_0835 at 968.752 kb on - strand, within Shewana3_0835 at 968.867 kb on + strand, within Shewana3_0835
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 966,018 + Shewana3_0833 0.18 +1.2 966,490 + Shewana3_0833 0.66 -2.3 966,490 + Shewana3_0833 0.66 -0.2 966,498 - Shewana3_0833 0.67 -1.1 966,498 - Shewana3_0833 0.67 -1.1 966,498 - Shewana3_0833 0.67 +0.7 966,498 - Shewana3_0833 0.67 -1.1 966,558 - Shewana3_0833 0.73 -1.4 966,625 - Shewana3_0833 0.80 -1.8 966,810 - +1.1 966,816 - +1.0 966,862 + +0.6 966,932 + +0.2 966,983 + -1.2 967,064 - Shewana3_0834 0.16 +0.9 967,289 + Shewana3_0834 0.39 +0.5 967,297 - Shewana3_0834 0.40 -1.3 967,381 + Shewana3_0834 0.49 +1.2 967,389 - Shewana3_0834 0.50 +0.6 967,389 - Shewana3_0834 0.50 +0.3 967,393 + Shewana3_0834 0.50 +0.7 967,480 - Shewana3_0834 0.59 -1.2 967,634 - Shewana3_0834 0.75 -0.3 967,646 + Shewana3_0834 0.76 +0.1 967,646 + Shewana3_0834 0.76 +0.5 967,646 + Shewana3_0834 0.76 +1.2 967,654 - Shewana3_0834 0.77 -2.4 967,749 + Shewana3_0834 0.87 -0.6 967,757 - Shewana3_0834 0.88 +1.6 967,781 + -2.0 968,067 + +0.6 968,196 - Shewana3_0835 0.20 +1.0 968,237 - Shewana3_0835 0.24 -1.3 968,258 + Shewana3_0835 0.26 +0.4 968,313 + Shewana3_0835 0.32 +0.3 968,401 + Shewana3_0835 0.40 -0.2 968,409 - Shewana3_0835 0.41 +0.4 968,578 - Shewana3_0835 0.58 +0.1 968,744 + Shewana3_0835 0.75 -1.2 968,752 - Shewana3_0835 0.76 +1.5 968,867 + Shewana3_0835 0.88 -1.8
Or see this region's nucleotide sequence