Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0832 and Shewana3_0833 are separated by 102 nucleotides Shewana3_0833 and Shewana3_0834 are separated by 98 nucleotides
Shewana3_0832: Shewana3_0832 - glutamate-ammonia ligase adenylyltransferase (RefSeq), at 962,879 to 965,743
_0832
Shewana3_0833: Shewana3_0833 - diguanylate cyclase with GAF sensor (RefSeq), at 965,846 to 966,814
_0833
Shewana3_0834: Shewana3_0834 - hypothetical protein (RefSeq), at 966,913 to 967,872
_0834
Position (kb) 965
966
967 Strain fitness (log2 ratio) -2
-1
0
1 at 964.953 kb on - strand, within Shewana3_0832 at 965.119 kb on - strand, within Shewana3_0832 at 965.159 kb on + strand, within Shewana3_0832 at 965.242 kb on + strand, within Shewana3_0832 at 965.242 kb on + strand, within Shewana3_0832 at 965.323 kb on + strand, within Shewana3_0832 at 965.517 kb on + strand at 965.525 kb on - strand at 965.590 kb on + strand at 965.610 kb on - strand at 966.018 kb on + strand, within Shewana3_0833 at 966.490 kb on + strand, within Shewana3_0833 at 966.490 kb on + strand, within Shewana3_0833 at 966.498 kb on - strand, within Shewana3_0833 at 966.498 kb on - strand, within Shewana3_0833 at 966.498 kb on - strand, within Shewana3_0833 at 966.498 kb on - strand, within Shewana3_0833 at 966.558 kb on - strand, within Shewana3_0833 at 966.625 kb on - strand, within Shewana3_0833 at 966.810 kb on - strand at 966.816 kb on - strand at 966.862 kb on + strand at 966.932 kb on + strand at 966.983 kb on + strand at 967.064 kb on - strand, within Shewana3_0834 at 967.289 kb on + strand, within Shewana3_0834 at 967.297 kb on - strand, within Shewana3_0834 at 967.381 kb on + strand, within Shewana3_0834 at 967.389 kb on - strand, within Shewana3_0834 at 967.389 kb on - strand, within Shewana3_0834 at 967.393 kb on + strand, within Shewana3_0834 at 967.480 kb on - strand, within Shewana3_0834 at 967.634 kb on - strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.654 kb on - strand, within Shewana3_0834 at 967.749 kb on + strand, within Shewana3_0834 at 967.757 kb on - strand, within Shewana3_0834 at 967.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 964,953 - Shewana3_0832 0.72 +0.5 965,119 - Shewana3_0832 0.78 +0.5 965,159 + Shewana3_0832 0.80 +0.3 965,242 + Shewana3_0832 0.82 -1.5 965,242 + Shewana3_0832 0.82 +1.0 965,323 + Shewana3_0832 0.85 -1.0 965,517 + +0.5 965,525 - -0.7 965,590 + +0.1 965,610 - +0.1 966,018 + Shewana3_0833 0.18 +1.2 966,490 + Shewana3_0833 0.66 -2.3 966,490 + Shewana3_0833 0.66 -0.2 966,498 - Shewana3_0833 0.67 -1.1 966,498 - Shewana3_0833 0.67 -1.1 966,498 - Shewana3_0833 0.67 +0.7 966,498 - Shewana3_0833 0.67 -1.1 966,558 - Shewana3_0833 0.73 -1.4 966,625 - Shewana3_0833 0.80 -1.8 966,810 - +1.1 966,816 - +1.0 966,862 + +0.6 966,932 + +0.2 966,983 + -1.2 967,064 - Shewana3_0834 0.16 +0.9 967,289 + Shewana3_0834 0.39 +0.5 967,297 - Shewana3_0834 0.40 -1.3 967,381 + Shewana3_0834 0.49 +1.2 967,389 - Shewana3_0834 0.50 +0.6 967,389 - Shewana3_0834 0.50 +0.3 967,393 + Shewana3_0834 0.50 +0.7 967,480 - Shewana3_0834 0.59 -1.2 967,634 - Shewana3_0834 0.75 -0.3 967,646 + Shewana3_0834 0.76 +0.1 967,646 + Shewana3_0834 0.76 +0.5 967,646 + Shewana3_0834 0.76 +1.2 967,654 - Shewana3_0834 0.77 -2.4 967,749 + Shewana3_0834 0.87 -0.6 967,757 - Shewana3_0834 0.88 +1.6 967,781 + -2.0
Or see this region's nucleotide sequence