Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0778 and Shewana3_0779 overlap by 4 nucleotides Shewana3_0779 and Shewana3_0780 are separated by 295 nucleotides Shewana3_0780 and Shewana3_0781 overlap by 1 nucleotides Shewana3_0781 and Shewana3_0782 are separated by 162 nucleotides
Shewana3_0778: Shewana3_0778 - ammonium transporter (RefSeq), at 900,554 to 901,954
_0778
Shewana3_0779: Shewana3_0779 - nitrogen regulatory protein P-II (RefSeq), at 901,951 to 902,289
_0779
Shewana3_0780: Shewana3_0780 - 2-dehydropantoate 2-reductase (RefSeq), at 902,585 to 903,568
_0780
Shewana3_0781: Shewana3_0781 - VanZ family protein (RefSeq), at 903,568 to 903,930
_0781
Shewana3_0782: Shewana3_0782 - putative nucleotide-binding protein (RefSeq), at 904,093 to 904,578
_0782
Position (kb) 902
903
904 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 901.613 kb on + strand, within Shewana3_0778 at 901.613 kb on + strand, within Shewana3_0778 at 901.618 kb on + strand, within Shewana3_0778 at 901.618 kb on + strand, within Shewana3_0778 at 901.700 kb on - strand, within Shewana3_0778 at 901.715 kb on - strand, within Shewana3_0778 at 901.798 kb on - strand, within Shewana3_0778 at 902.155 kb on - strand, within Shewana3_0779 at 902.186 kb on - strand, within Shewana3_0779 at 902.712 kb on + strand, within Shewana3_0780 at 902.730 kb on - strand, within Shewana3_0780 at 902.788 kb on + strand, within Shewana3_0780 at 902.912 kb on - strand, within Shewana3_0780 at 902.970 kb on - strand, within Shewana3_0780 at 902.991 kb on + strand, within Shewana3_0780 at 902.993 kb on + strand, within Shewana3_0780 at 903.003 kb on + strand, within Shewana3_0780 at 903.003 kb on + strand, within Shewana3_0780 at 903.011 kb on - strand, within Shewana3_0780 at 903.011 kb on - strand, within Shewana3_0780 at 903.011 kb on - strand, within Shewana3_0780 at 903.079 kb on - strand, within Shewana3_0780 at 903.144 kb on - strand, within Shewana3_0780 at 903.184 kb on - strand, within Shewana3_0780 at 903.537 kb on - strand at 903.620 kb on - strand, within Shewana3_0781 at 903.687 kb on - strand, within Shewana3_0781 at 903.818 kb on - strand, within Shewana3_0781 at 904.074 kb on + strand at 904.486 kb on + strand, within Shewana3_0782 at 904.542 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 901,613 + Shewana3_0778 0.76 +0.0 901,613 + Shewana3_0778 0.76 +0.6 901,618 + Shewana3_0778 0.76 +0.5 901,618 + Shewana3_0778 0.76 +0.5 901,700 - Shewana3_0778 0.82 -0.3 901,715 - Shewana3_0778 0.83 +1.0 901,798 - Shewana3_0778 0.89 -0.1 902,155 - Shewana3_0779 0.60 +0.6 902,186 - Shewana3_0779 0.69 +0.5 902,712 + Shewana3_0780 0.13 -0.1 902,730 - Shewana3_0780 0.15 -1.1 902,788 + Shewana3_0780 0.21 -1.9 902,912 - Shewana3_0780 0.33 -3.4 902,970 - Shewana3_0780 0.39 -3.3 902,991 + Shewana3_0780 0.41 -2.2 902,993 + Shewana3_0780 0.41 -2.9 903,003 + Shewana3_0780 0.42 -5.2 903,003 + Shewana3_0780 0.42 -0.9 903,011 - Shewana3_0780 0.43 -3.3 903,011 - Shewana3_0780 0.43 -3.7 903,011 - Shewana3_0780 0.43 -4.1 903,079 - Shewana3_0780 0.50 -3.8 903,144 - Shewana3_0780 0.57 -2.8 903,184 - Shewana3_0780 0.61 -3.3 903,537 - -3.4 903,620 - Shewana3_0781 0.14 -2.2 903,687 - Shewana3_0781 0.33 +0.9 903,818 - Shewana3_0781 0.69 +0.9 904,074 + +0.1 904,486 + Shewana3_0782 0.81 +0.9 904,542 + +0.5
Or see this region's nucleotide sequence