Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0572 and Shewana3_0573 are separated by 65 nucleotides Shewana3_0573 and Shewana3_0574 are separated by 238 nucleotides
Shewana3_0572: Shewana3_0572 - enoyl-CoA hydratase (RefSeq), at 664,860 to 665,666
_0572
Shewana3_0573: Shewana3_0573 - hypothetical protein (RefSeq), at 665,732 to 666,691
_0573
Shewana3_0574: Shewana3_0574 - ATP-dependent helicase HepA (RefSeq), at 666,930 to 669,836
_0574
Position (kb) 665
666
667 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 664.903 kb on + strand at 664.911 kb on - strand at 665.159 kb on + strand, within Shewana3_0572 at 665.159 kb on + strand, within Shewana3_0572 at 665.186 kb on + strand, within Shewana3_0572 at 665.192 kb on - strand, within Shewana3_0572 at 665.295 kb on + strand, within Shewana3_0572 at 665.544 kb on + strand, within Shewana3_0572 at 665.544 kb on + strand, within Shewana3_0572 at 665.546 kb on + strand, within Shewana3_0572 at 665.552 kb on - strand, within Shewana3_0572 at 665.552 kb on - strand, within Shewana3_0572 at 665.552 kb on - strand, within Shewana3_0572 at 665.569 kb on + strand, within Shewana3_0572 at 665.772 kb on - strand at 665.846 kb on + strand, within Shewana3_0573 at 666.221 kb on + strand, within Shewana3_0573 at 666.229 kb on - strand, within Shewana3_0573 at 666.304 kb on + strand, within Shewana3_0573 at 666.312 kb on - strand, within Shewana3_0573 at 666.437 kb on + strand, within Shewana3_0573 at 666.437 kb on + strand, within Shewana3_0573 at 666.581 kb on - strand, within Shewana3_0573 at 666.670 kb on - strand at 666.696 kb on - strand at 666.815 kb on - strand at 666.837 kb on - strand at 666.927 kb on + strand at 666.935 kb on - strand at 667.477 kb on - strand, within Shewana3_0574 at 667.478 kb on - strand, within Shewana3_0574
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 664,903 + +0.0 664,911 - -1.3 665,159 + Shewana3_0572 0.37 +1.0 665,159 + Shewana3_0572 0.37 +0.7 665,186 + Shewana3_0572 0.40 -1.7 665,192 - Shewana3_0572 0.41 +0.0 665,295 + Shewana3_0572 0.54 +0.2 665,544 + Shewana3_0572 0.85 +0.5 665,544 + Shewana3_0572 0.85 -2.2 665,546 + Shewana3_0572 0.85 -1.0 665,552 - Shewana3_0572 0.86 -0.3 665,552 - Shewana3_0572 0.86 +0.7 665,552 - Shewana3_0572 0.86 +1.0 665,569 + Shewana3_0572 0.88 -0.5 665,772 - +1.3 665,846 + Shewana3_0573 0.12 -2.2 666,221 + Shewana3_0573 0.51 +0.5 666,229 - Shewana3_0573 0.52 +0.1 666,304 + Shewana3_0573 0.60 -0.4 666,312 - Shewana3_0573 0.60 +3.2 666,437 + Shewana3_0573 0.73 +0.7 666,437 + Shewana3_0573 0.73 -1.1 666,581 - Shewana3_0573 0.88 -1.8 666,670 - +0.1 666,696 - +0.2 666,815 - +0.2 666,837 - +0.2 666,927 + -1.1 666,935 - -1.4 667,477 - Shewana3_0574 0.19 -0.5 667,478 - Shewana3_0574 0.19 -1.3
Or see this region's nucleotide sequence