Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0556 and Shewana3_0557 are separated by 142 nucleotides Shewana3_0557 and Shewana3_0558 are separated by 364 nucleotides
Shewana3_0556: Shewana3_0556 - GumN family protein (RefSeq), at 648,950 to 649,846
_0556
Shewana3_0557: Shewana3_0557 - methyltransferase type 11 (RefSeq), at 649,989 to 650,774
_0557
Shewana3_0558: Shewana3_0558 - dehydratase (RefSeq), at 651,139 to 652,002
_0558
Position (kb) 649
650
651 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 649.015 kb on + strand at 649.053 kb on - strand, within Shewana3_0556 at 649.172 kb on - strand, within Shewana3_0556 at 649.308 kb on + strand, within Shewana3_0556 at 649.328 kb on + strand, within Shewana3_0556 at 649.423 kb on - strand, within Shewana3_0556 at 649.438 kb on + strand, within Shewana3_0556 at 649.481 kb on + strand, within Shewana3_0556 at 649.515 kb on + strand, within Shewana3_0556 at 649.567 kb on + strand, within Shewana3_0556 at 649.654 kb on + strand, within Shewana3_0556 at 649.841 kb on + strand at 649.920 kb on - strand at 650.075 kb on - strand, within Shewana3_0557 at 650.161 kb on - strand, within Shewana3_0557 at 650.263 kb on - strand, within Shewana3_0557 at 650.263 kb on - strand, within Shewana3_0557 at 650.355 kb on - strand, within Shewana3_0557 at 650.577 kb on + strand, within Shewana3_0557 at 650.585 kb on - strand, within Shewana3_0557 at 650.719 kb on + strand at 650.784 kb on - strand at 650.786 kb on + strand at 650.978 kb on + strand at 650.978 kb on + strand at 650.980 kb on - strand at 650.986 kb on - strand at 651.136 kb on + strand at 651.191 kb on - strand at 651.237 kb on - strand, within Shewana3_0558 at 651.240 kb on - strand, within Shewana3_0558 at 651.339 kb on + strand, within Shewana3_0558 at 651.728 kb on + strand, within Shewana3_0558 at 651.767 kb on - strand, within Shewana3_0558
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 649,015 + -0.2 649,053 - Shewana3_0556 0.11 +1.3 649,172 - Shewana3_0556 0.25 +0.6 649,308 + Shewana3_0556 0.40 +0.4 649,328 + Shewana3_0556 0.42 -2.3 649,423 - Shewana3_0556 0.53 -1.2 649,438 + Shewana3_0556 0.54 +0.2 649,481 + Shewana3_0556 0.59 -1.9 649,515 + Shewana3_0556 0.63 +0.2 649,567 + Shewana3_0556 0.69 -0.2 649,654 + Shewana3_0556 0.78 +0.5 649,841 + +0.4 649,920 - +0.2 650,075 - Shewana3_0557 0.11 -0.6 650,161 - Shewana3_0557 0.22 -0.2 650,263 - Shewana3_0557 0.35 -0.4 650,263 - Shewana3_0557 0.35 -1.5 650,355 - Shewana3_0557 0.47 -1.8 650,577 + Shewana3_0557 0.75 -2.1 650,585 - Shewana3_0557 0.76 -1.4 650,719 + +0.8 650,784 - -2.9 650,786 + -2.0 650,978 + -2.2 650,978 + +0.4 650,980 - +0.8 650,986 - +1.1 651,136 + +0.7 651,191 - +0.7 651,237 - Shewana3_0558 0.11 -0.8 651,240 - Shewana3_0558 0.12 -2.1 651,339 + Shewana3_0558 0.23 +1.1 651,728 + Shewana3_0558 0.68 +1.0 651,767 - Shewana3_0558 0.73 +1.4
Or see this region's nucleotide sequence