Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0531 and Shewana3_0532 are separated by 500 nucleotides Shewana3_0532 and Shewana3_0533 are separated by 40 nucleotides Shewana3_0533 and Shewana3_0534 are separated by 65 nucleotides
Shewana3_0531: Shewana3_0531 - lysine exporter protein LysE/YggA (RefSeq), at 625,299 to 625,910
_0531
Shewana3_0532: Shewana3_0532 - ArsR family transcriptional regulator (RefSeq), at 626,411 to 626,755
_0532
Shewana3_0533: Shewana3_0533 - bile acid:sodium symporter (RefSeq), at 626,796 to 627,815
_0533
Shewana3_0534: Shewana3_0534 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 627,881 to 628,894
_0534
Position (kb) 626
627
628 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 625.796 kb on - strand, within Shewana3_0531 at 625.808 kb on + strand, within Shewana3_0531 at 625.854 kb on - strand at 625.856 kb on + strand at 625.856 kb on + strand at 625.856 kb on + strand at 625.856 kb on + strand at 626.033 kb on + strand at 626.052 kb on + strand at 626.053 kb on - strand at 626.107 kb on - strand at 626.217 kb on - strand at 626.455 kb on + strand, within Shewana3_0532 at 626.463 kb on - strand, within Shewana3_0532 at 626.535 kb on - strand, within Shewana3_0532 at 626.672 kb on - strand, within Shewana3_0532 at 626.736 kb on - strand at 626.827 kb on + strand at 626.848 kb on + strand at 626.964 kb on - strand, within Shewana3_0533 at 627.226 kb on + strand, within Shewana3_0533 at 627.779 kb on - strand at 627.932 kb on - strand at 628.165 kb on - strand, within Shewana3_0534 at 628.172 kb on - strand, within Shewana3_0534 at 628.299 kb on - strand, within Shewana3_0534 at 628.407 kb on + strand, within Shewana3_0534 at 628.546 kb on + strand, within Shewana3_0534 at 628.552 kb on + strand, within Shewana3_0534 at 628.654 kb on - strand, within Shewana3_0534 at 628.724 kb on - strand, within Shewana3_0534 at 628.798 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 625,796 - Shewana3_0531 0.81 -3.3 625,808 + Shewana3_0531 0.83 -1.5 625,854 - +0.7 625,856 + +0.5 625,856 + +0.5 625,856 + +0.2 625,856 + -2.4 626,033 + -0.1 626,052 + +0.2 626,053 - -0.3 626,107 - -0.8 626,217 - +0.9 626,455 + Shewana3_0532 0.13 +0.4 626,463 - Shewana3_0532 0.15 -0.8 626,535 - Shewana3_0532 0.36 +1.0 626,672 - Shewana3_0532 0.76 -1.3 626,736 - +0.2 626,827 + +0.7 626,848 + -0.7 626,964 - Shewana3_0533 0.16 +0.5 627,226 + Shewana3_0533 0.42 +0.4 627,779 - -0.5 627,932 - -3.2 628,165 - Shewana3_0534 0.28 +0.2 628,172 - Shewana3_0534 0.29 +0.4 628,299 - Shewana3_0534 0.41 +0.6 628,407 + Shewana3_0534 0.52 -1.8 628,546 + Shewana3_0534 0.66 +0.6 628,552 + Shewana3_0534 0.66 +0.7 628,654 - Shewana3_0534 0.76 -1.9 628,724 - Shewana3_0534 0.83 +0.6 628,798 + +0.5
Or see this region's nucleotide sequence