Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0484 and Shewana3_0485 are separated by 44 nucleotides Shewana3_0485 and Shewana3_0486 are separated by 255 nucleotides Shewana3_0486 and Shewana3_0487 are separated by 49 nucleotides Shewana3_0487 and Shewana3_0488 overlap by 4 nucleotides
Shewana3_0484: Shewana3_0484 - XRE family transcriptional regulator (RefSeq), at 567,175 to 567,408
_0484
Shewana3_0485: Shewana3_0485 - hypothetical protein (RefSeq), at 567,453 to 568,031
_0485
Shewana3_0486: Shewana3_0486 - redoxin domain-containing protein (RefSeq), at 568,287 to 568,871
_0486
Shewana3_0487: Shewana3_0487 - cytochrome C biogenesis protein (RefSeq), at 568,921 to 569,340
_0487
Shewana3_0488: Shewana3_0488 - cytochrome c-type biogenesis protein CcmF (RefSeq), at 569,337 to 571,376
_0488
Position (kb) 568
569 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 567.297 kb on + strand, within Shewana3_0484 at 567.415 kb on + strand at 567.415 kb on + strand at 567.415 kb on + strand at 567.422 kb on + strand at 567.484 kb on + strand at 567.497 kb on - strand at 567.532 kb on - strand, within Shewana3_0485 at 567.735 kb on - strand, within Shewana3_0485 at 567.761 kb on - strand, within Shewana3_0485 at 568.423 kb on + strand, within Shewana3_0486 at 568.431 kb on - strand, within Shewana3_0486 at 568.480 kb on - strand, within Shewana3_0486 at 568.628 kb on + strand, within Shewana3_0486 at 568.658 kb on + strand, within Shewana3_0486 at 568.658 kb on + strand, within Shewana3_0486 at 568.666 kb on - strand, within Shewana3_0486 at 568.666 kb on - strand, within Shewana3_0486 at 568.666 kb on - strand, within Shewana3_0486 at 568.754 kb on + strand, within Shewana3_0486 at 568.803 kb on + strand, within Shewana3_0486 at 568.818 kb on + strand at 568.873 kb on + strand at 568.911 kb on + strand at 568.918 kb on + strand at 568.950 kb on - strand at 569.164 kb on + strand, within Shewana3_0487 at 569.169 kb on + strand, within Shewana3_0487 at 569.172 kb on - strand, within Shewana3_0487 at 569.177 kb on - strand, within Shewana3_0487 at 569.406 kb on - strand at 569.530 kb on - strand at 569.627 kb on + strand, within Shewana3_0488 at 569.634 kb on - strand, within Shewana3_0488 at 569.639 kb on - strand, within Shewana3_0488 at 569.767 kb on - strand, within Shewana3_0488 at 569.767 kb on - strand, within Shewana3_0488 at 569.775 kb on + strand, within Shewana3_0488
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 567,297 + Shewana3_0484 0.52 +0.3 567,415 + +0.5 567,415 + +0.5 567,415 + +1.0 567,422 + -1.4 567,484 + +0.5 567,497 - +0.9 567,532 - Shewana3_0485 0.14 +0.6 567,735 - Shewana3_0485 0.49 +1.1 567,761 - Shewana3_0485 0.53 +0.4 568,423 + Shewana3_0486 0.23 +0.5 568,431 - Shewana3_0486 0.25 +1.2 568,480 - Shewana3_0486 0.33 -3.3 568,628 + Shewana3_0486 0.58 -1.4 568,658 + Shewana3_0486 0.63 +0.5 568,658 + Shewana3_0486 0.63 +0.3 568,666 - Shewana3_0486 0.65 -0.9 568,666 - Shewana3_0486 0.65 -1.2 568,666 - Shewana3_0486 0.65 +0.2 568,754 + Shewana3_0486 0.80 +1.3 568,803 + Shewana3_0486 0.88 +1.1 568,818 + +1.9 568,873 + +0.9 568,911 + -0.8 568,918 + +0.9 568,950 - -0.2 569,164 + Shewana3_0487 0.58 -2.2 569,169 + Shewana3_0487 0.59 +1.3 569,172 - Shewana3_0487 0.60 +0.3 569,177 - Shewana3_0487 0.61 -1.5 569,406 - -0.5 569,530 - +0.4 569,627 + Shewana3_0488 0.14 +0.7 569,634 - Shewana3_0488 0.15 +1.1 569,639 - Shewana3_0488 0.15 +0.4 569,767 - Shewana3_0488 0.21 -0.5 569,767 - Shewana3_0488 0.21 -2.9 569,775 + Shewana3_0488 0.21 +0.6
Or see this region's nucleotide sequence