Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0482 and Shewana3_0483 are separated by 157 nucleotides Shewana3_0483 and Shewana3_0484 overlap by 4 nucleotides Shewana3_0484 and Shewana3_0485 are separated by 44 nucleotides Shewana3_0485 and Shewana3_0486 are separated by 255 nucleotides Shewana3_0486 and Shewana3_0487 are separated by 49 nucleotides
Shewana3_0482: Shewana3_0482 - peptidase S9 prolyl oligopeptidase (RefSeq), at 564,442 to 566,502
_0482
Shewana3_0483: Shewana3_0483 - hypothetical protein (RefSeq), at 566,660 to 567,178
_0483
Shewana3_0484: Shewana3_0484 - XRE family transcriptional regulator (RefSeq), at 567,175 to 567,408
_0484
Shewana3_0485: Shewana3_0485 - hypothetical protein (RefSeq), at 567,453 to 568,031
_0485
Shewana3_0486: Shewana3_0486 - redoxin domain-containing protein (RefSeq), at 568,287 to 568,871
_0486
Shewana3_0487: Shewana3_0487 - cytochrome C biogenesis protein (RefSeq), at 568,921 to 569,340
_0487
Position (kb) 567
568
569 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 566.470 kb on + strand at 566.632 kb on - strand at 566.642 kb on - strand at 567.130 kb on + strand at 567.243 kb on + strand, within Shewana3_0484 at 567.251 kb on - strand, within Shewana3_0484 at 567.251 kb on - strand, within Shewana3_0484 at 567.251 kb on - strand, within Shewana3_0484 at 567.297 kb on + strand, within Shewana3_0484 at 567.415 kb on + strand at 567.415 kb on + strand at 567.415 kb on + strand at 567.422 kb on + strand at 567.484 kb on + strand at 567.497 kb on - strand at 567.532 kb on - strand, within Shewana3_0485 at 567.735 kb on - strand, within Shewana3_0485 at 567.761 kb on - strand, within Shewana3_0485 at 568.423 kb on + strand, within Shewana3_0486 at 568.431 kb on - strand, within Shewana3_0486 at 568.480 kb on - strand, within Shewana3_0486 at 568.628 kb on + strand, within Shewana3_0486 at 568.658 kb on + strand, within Shewana3_0486 at 568.658 kb on + strand, within Shewana3_0486 at 568.666 kb on - strand, within Shewana3_0486 at 568.666 kb on - strand, within Shewana3_0486 at 568.666 kb on - strand, within Shewana3_0486 at 568.754 kb on + strand, within Shewana3_0486 at 568.803 kb on + strand, within Shewana3_0486 at 568.818 kb on + strand at 568.873 kb on + strand at 568.911 kb on + strand at 568.918 kb on + strand at 568.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 566,470 + -2.3 566,632 - -1.7 566,642 - -1.2 567,130 + -1.2 567,243 + Shewana3_0484 0.29 +0.3 567,251 - Shewana3_0484 0.32 +0.9 567,251 - Shewana3_0484 0.32 +0.3 567,251 - Shewana3_0484 0.32 +0.5 567,297 + Shewana3_0484 0.52 +0.3 567,415 + +0.5 567,415 + +0.5 567,415 + +1.0 567,422 + -1.4 567,484 + +0.5 567,497 - +0.9 567,532 - Shewana3_0485 0.14 +0.6 567,735 - Shewana3_0485 0.49 +1.1 567,761 - Shewana3_0485 0.53 +0.4 568,423 + Shewana3_0486 0.23 +0.5 568,431 - Shewana3_0486 0.25 +1.2 568,480 - Shewana3_0486 0.33 -3.3 568,628 + Shewana3_0486 0.58 -1.4 568,658 + Shewana3_0486 0.63 +0.5 568,658 + Shewana3_0486 0.63 +0.3 568,666 - Shewana3_0486 0.65 -0.9 568,666 - Shewana3_0486 0.65 -1.2 568,666 - Shewana3_0486 0.65 +0.2 568,754 + Shewana3_0486 0.80 +1.3 568,803 + Shewana3_0486 0.88 +1.1 568,818 + +1.9 568,873 + +0.9 568,911 + -0.8 568,918 + +0.9 568,950 - -0.2
Or see this region's nucleotide sequence