Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0444 and Shewana3_0445 are separated by 346 nucleotides Shewana3_0445 and Shewana3_0446 are separated by 3 nucleotides Shewana3_0446 and Shewana3_0447 are separated by 6 nucleotides Shewana3_0447 and Shewana3_0448 are separated by 52 nucleotides
Shewana3_0444: Shewana3_0444 - band 7 protein (RefSeq), at 519,799 to 520,686
_0444
Shewana3_0445: Shewana3_0445 - hypothetical protein (RefSeq), at 521,033 to 521,287
_0445
Shewana3_0446: Shewana3_0446 - hypothetical protein (RefSeq), at 521,291 to 521,581
_0446
Shewana3_0447: Shewana3_0447 - response regulator receiver protein (RefSeq), at 521,588 to 523,207
_0447
Shewana3_0448: Shewana3_0448 - major facilitator transporter (RefSeq), at 523,260 to 524,432
_0448
Position (kb) 521
522
523
524 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 521.000 kb on + strand at 521.159 kb on - strand, within Shewana3_0445 at 521.193 kb on + strand, within Shewana3_0445 at 521.253 kb on - strand, within Shewana3_0445 at 521.343 kb on - strand, within Shewana3_0446 at 521.369 kb on + strand, within Shewana3_0446 at 521.369 kb on + strand, within Shewana3_0446 at 521.375 kb on + strand, within Shewana3_0446 at 521.375 kb on + strand, within Shewana3_0446 at 521.377 kb on - strand, within Shewana3_0446 at 521.438 kb on - strand, within Shewana3_0446 at 521.571 kb on - strand at 521.627 kb on + strand at 521.698 kb on + strand at 521.729 kb on - strand at 521.808 kb on - strand, within Shewana3_0447 at 522.044 kb on + strand, within Shewana3_0447 at 522.090 kb on + strand, within Shewana3_0447 at 522.140 kb on - strand, within Shewana3_0447 at 522.320 kb on + strand, within Shewana3_0447 at 522.342 kb on - strand, within Shewana3_0447 at 522.343 kb on - strand, within Shewana3_0447 at 522.458 kb on + strand, within Shewana3_0447 at 522.469 kb on + strand, within Shewana3_0447 at 522.477 kb on - strand, within Shewana3_0447 at 522.531 kb on - strand, within Shewana3_0447 at 522.547 kb on - strand, within Shewana3_0447 at 522.745 kb on - strand, within Shewana3_0447 at 523.184 kb on + strand at 523.467 kb on + strand, within Shewana3_0448 at 523.509 kb on - strand, within Shewana3_0448 at 523.532 kb on + strand, within Shewana3_0448 at 523.661 kb on + strand, within Shewana3_0448 at 523.667 kb on - strand, within Shewana3_0448 at 523.667 kb on - strand, within Shewana3_0448 at 523.747 kb on + strand, within Shewana3_0448 at 523.815 kb on - strand, within Shewana3_0448
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 521,000 + -2.2 521,159 - Shewana3_0445 0.49 -0.7 521,193 + Shewana3_0445 0.63 +0.6 521,253 - Shewana3_0445 0.86 -1.3 521,343 - Shewana3_0446 0.18 -1.2 521,369 + Shewana3_0446 0.27 +1.2 521,369 + Shewana3_0446 0.27 +0.3 521,375 + Shewana3_0446 0.29 +0.6 521,375 + Shewana3_0446 0.29 +0.3 521,377 - Shewana3_0446 0.30 -1.6 521,438 - Shewana3_0446 0.51 +1.2 521,571 - -0.4 521,627 + +0.2 521,698 + -0.5 521,729 - +0.5 521,808 - Shewana3_0447 0.14 +0.7 522,044 + Shewana3_0447 0.28 +0.4 522,090 + Shewana3_0447 0.31 +0.6 522,140 - Shewana3_0447 0.34 +1.2 522,320 + Shewana3_0447 0.45 -1.8 522,342 - Shewana3_0447 0.47 -2.8 522,343 - Shewana3_0447 0.47 -1.3 522,458 + Shewana3_0447 0.54 +0.4 522,469 + Shewana3_0447 0.54 +0.6 522,477 - Shewana3_0447 0.55 +0.2 522,531 - Shewana3_0447 0.58 +0.7 522,547 - Shewana3_0447 0.59 +0.4 522,745 - Shewana3_0447 0.71 +0.7 523,184 + +0.4 523,467 + Shewana3_0448 0.18 -0.0 523,509 - Shewana3_0448 0.21 +0.4 523,532 + Shewana3_0448 0.23 +0.2 523,661 + Shewana3_0448 0.34 -1.3 523,667 - Shewana3_0448 0.35 +0.5 523,667 - Shewana3_0448 0.35 -0.9 523,747 + Shewana3_0448 0.42 +0.5 523,815 - Shewana3_0448 0.47 +0.5
Or see this region's nucleotide sequence