Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceE and Shewana3_0427 are separated by 12 nucleotides Shewana3_0427 and Shewana3_0428 are separated by 149 nucleotides
Shewana3_0426: aceE - pyruvate dehydrogenase subunit E1 (RefSeq), at 485,256 to 487,922
aceE
Shewana3_0427: Shewana3_0427 - pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (RefSeq), at 487,935 to 489,941
_0427
Shewana3_0428: Shewana3_0428 - dihydrolipoamide dehydrogenase (RefSeq), at 490,091 to 491,518
_0428
Position (kb) 487
488
489
490 Strain fitness (log2 ratio) -1
0
1
2
3 at 487.017 kb on + strand, within aceE at 487.088 kb on + strand, within aceE at 487.096 kb on - strand, within aceE at 487.192 kb on - strand, within aceE at 487.210 kb on - strand, within aceE at 487.230 kb on - strand, within aceE at 487.493 kb on - strand, within aceE at 487.679 kb on + strand at 487.717 kb on + strand at 487.725 kb on + strand at 487.733 kb on - strand at 487.780 kb on + strand at 487.800 kb on - strand at 487.851 kb on + strand at 487.991 kb on - strand at 488.097 kb on + strand at 488.193 kb on + strand, within Shewana3_0427 at 488.340 kb on + strand, within Shewana3_0427 at 488.506 kb on + strand, within Shewana3_0427 at 488.514 kb on - strand, within Shewana3_0427 at 488.725 kb on + strand, within Shewana3_0427 at 488.733 kb on - strand, within Shewana3_0427 at 488.835 kb on + strand, within Shewana3_0427 at 488.914 kb on - strand, within Shewana3_0427 at 489.196 kb on - strand, within Shewana3_0427 at 489.299 kb on - strand, within Shewana3_0427 at 489.307 kb on + strand, within Shewana3_0427 at 489.363 kb on - strand, within Shewana3_0427 at 489.472 kb on - strand at 489.486 kb on + strand, within Shewana3_0427 at 489.546 kb on - strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.570 kb on - strand, within Shewana3_0427 at 489.659 kb on + strand, within Shewana3_0427 at 489.675 kb on + strand, within Shewana3_0427
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 487,017 + aceE Shewana3_0426 0.66 +3.2 487,088 + aceE Shewana3_0426 0.69 +2.6 487,096 - aceE Shewana3_0426 0.69 +3.4 487,192 - aceE Shewana3_0426 0.73 +3.2 487,210 - aceE Shewana3_0426 0.73 +3.0 487,230 - aceE Shewana3_0426 0.74 +3.0 487,493 - aceE Shewana3_0426 0.84 +2.5 487,679 + +1.1 487,717 + +3.5 487,725 + +0.8 487,733 - +0.9 487,780 + +3.2 487,800 - +0.7 487,851 + +1.3 487,991 - +2.5 488,097 + +3.2 488,193 + Shewana3_0427 0.13 +2.8 488,340 + Shewana3_0427 0.20 +0.5 488,506 + Shewana3_0427 0.28 +3.3 488,514 - Shewana3_0427 0.29 +1.1 488,725 + Shewana3_0427 0.39 +2.7 488,733 - Shewana3_0427 0.40 +1.5 488,835 + Shewana3_0427 0.45 +1.0 488,914 - Shewana3_0427 0.49 +2.9 489,196 - Shewana3_0427 0.63 +2.6 489,299 - Shewana3_0427 0.68 +2.5 489,307 + Shewana3_0427 0.68 +0.5 489,363 - Shewana3_0427 0.71 +1.3 489,472 - +3.4 489,486 + Shewana3_0427 0.77 +1.9 489,546 - Shewana3_0427 0.80 +0.5 489,562 + Shewana3_0427 0.81 +2.6 489,562 + Shewana3_0427 0.81 +2.7 489,562 + Shewana3_0427 0.81 +2.3 489,570 - Shewana3_0427 0.81 +2.8 489,659 + Shewana3_0427 0.86 +0.4 489,675 + Shewana3_0427 0.87 +2.5
Or see this region's nucleotide sequence