Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0423 and Shewana3_0424 are separated by 184 nucleotides Shewana3_0424 and pdhR are separated by 325 nucleotides
Shewana3_0423: Shewana3_0423 - N-acetyl-anhydromuranmyl-L-alanine amidase (RefSeq), at 482,516 to 483,061
_0423
Shewana3_0424: Shewana3_0424 - regulatory protein AmpE (RefSeq), at 483,246 to 484,097
_0424
Shewana3_0425: pdhR - transcriptional regulator PdhR (RefSeq), at 484,423 to 485,175
pdhR
Position (kb) 483
484
485 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 482.438 kb on + strand at 482.474 kb on - strand at 482.518 kb on - strand at 482.539 kb on - strand at 482.624 kb on - strand, within Shewana3_0423 at 482.637 kb on + strand, within Shewana3_0423 at 482.637 kb on + strand, within Shewana3_0423 at 482.647 kb on + strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.655 kb on - strand, within Shewana3_0423 at 482.718 kb on + strand, within Shewana3_0423 at 482.762 kb on + strand, within Shewana3_0423 at 482.812 kb on - strand, within Shewana3_0423 at 482.951 kb on - strand, within Shewana3_0423 at 482.963 kb on - strand, within Shewana3_0423 at 482.990 kb on - strand, within Shewana3_0423 at 483.108 kb on + strand at 483.108 kb on + strand at 483.116 kb on - strand at 483.116 kb on - strand at 483.129 kb on + strand at 483.235 kb on + strand at 483.285 kb on + strand at 483.384 kb on - strand, within Shewana3_0424 at 483.732 kb on + strand, within Shewana3_0424 at 483.732 kb on + strand, within Shewana3_0424 at 483.737 kb on + strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.860 kb on + strand, within Shewana3_0424 at 483.872 kb on + strand, within Shewana3_0424 at 483.876 kb on + strand, within Shewana3_0424 at 483.892 kb on - strand, within Shewana3_0424 at 483.960 kb on + strand, within Shewana3_0424 at 484.039 kb on + strand at 484.039 kb on + strand at 484.039 kb on + strand at 484.047 kb on - strand at 484.165 kb on + strand at 484.233 kb on + strand at 484.513 kb on + strand, within pdhR at 484.918 kb on + strand, within pdhR
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 482,438 + -1.2 482,474 - +0.7 482,518 - +0.7 482,539 - -1.9 482,624 - Shewana3_0423 0.20 -1.9 482,637 + Shewana3_0423 0.22 -1.6 482,637 + Shewana3_0423 0.22 +0.9 482,647 + Shewana3_0423 0.24 -1.6 482,655 - Shewana3_0423 0.25 +0.5 482,655 - Shewana3_0423 0.25 -2.6 482,655 - Shewana3_0423 0.25 +0.5 482,655 - Shewana3_0423 0.25 -2.4 482,655 - Shewana3_0423 0.25 +0.1 482,655 - Shewana3_0423 0.25 +0.4 482,655 - Shewana3_0423 0.25 -1.9 482,718 + Shewana3_0423 0.37 -1.5 482,762 + Shewana3_0423 0.45 -4.9 482,812 - Shewana3_0423 0.54 -1.6 482,951 - Shewana3_0423 0.80 -1.4 482,963 - Shewana3_0423 0.82 -1.7 482,990 - Shewana3_0423 0.87 -2.0 483,108 + -1.1 483,108 + -0.2 483,116 - +0.6 483,116 - +0.4 483,129 + -1.0 483,235 + +0.7 483,285 + -0.2 483,384 - Shewana3_0424 0.16 -2.5 483,732 + Shewana3_0424 0.57 -1.5 483,732 + Shewana3_0424 0.57 +0.5 483,737 + Shewana3_0424 0.58 +0.9 483,740 - Shewana3_0424 0.58 +0.4 483,740 - Shewana3_0424 0.58 +0.5 483,740 - Shewana3_0424 0.58 -1.5 483,745 - Shewana3_0424 0.59 -2.1 483,745 - Shewana3_0424 0.59 +0.3 483,745 - Shewana3_0424 0.59 +0.5 483,860 + Shewana3_0424 0.72 +1.0 483,872 + Shewana3_0424 0.73 +1.3 483,876 + Shewana3_0424 0.74 +0.4 483,892 - Shewana3_0424 0.76 +0.3 483,960 + Shewana3_0424 0.84 +0.7 484,039 + +0.7 484,039 + +0.6 484,039 + -1.5 484,047 - +0.6 484,165 + +0.5 484,233 + +0.5 484,513 + pdhR Shewana3_0425 0.12 +3.9 484,918 + pdhR Shewana3_0425 0.66 +2.1
Or see this region's nucleotide sequence