Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0399 and prmA are separated by 174 nucleotides prmA and Shewana3_0401 are separated by 442 nucleotides
Shewana3_0399: Shewana3_0399 - nifR3 family TIM-barrel protein (RefSeq), at 457,826 to 458,794
_0399
Shewana3_0400: prmA - ribosomal protein L11 methyltransferase (RefSeq), at 458,969 to 459,850
prmA
Shewana3_0401: Shewana3_0401 - fumarate reductase cytochrome b-556 subunit (RefSeq), at 460,293 to 461,018
_0401
Position (kb) 458
459
460 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 458.191 kb on - strand, within Shewana3_0399 at 458.368 kb on - strand, within Shewana3_0399 at 458.463 kb on - strand, within Shewana3_0399 at 458.543 kb on - strand, within Shewana3_0399 at 458.692 kb on - strand, within Shewana3_0399 at 458.789 kb on - strand at 458.819 kb on + strand at 458.822 kb on - strand at 458.822 kb on - strand at 458.822 kb on - strand at 458.968 kb on - strand at 459.077 kb on - strand, within prmA at 459.143 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.576 kb on + strand, within prmA at 459.666 kb on + strand, within prmA at 459.751 kb on + strand, within prmA at 459.759 kb on - strand, within prmA at 459.759 kb on - strand, within prmA at 459.851 kb on + strand at 459.858 kb on + strand at 460.045 kb on + strand at 460.208 kb on - strand at 460.344 kb on - strand at 460.368 kb on + strand, within Shewana3_0401 at 460.387 kb on + strand, within Shewana3_0401 at 460.395 kb on - strand, within Shewana3_0401 at 460.395 kb on - strand, within Shewana3_0401 at 460.402 kb on + strand, within Shewana3_0401 at 460.501 kb on + strand, within Shewana3_0401 at 460.514 kb on - strand, within Shewana3_0401 at 460.514 kb on - strand, within Shewana3_0401 at 460.650 kb on - strand, within Shewana3_0401 at 460.729 kb on - strand, within Shewana3_0401 at 460.799 kb on + strand, within Shewana3_0401 at 460.809 kb on - strand, within Shewana3_0401
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 458,191 - Shewana3_0399 0.38 +2.5 458,368 - Shewana3_0399 0.56 +2.3 458,463 - Shewana3_0399 0.66 +2.4 458,543 - Shewana3_0399 0.74 +0.3 458,692 - Shewana3_0399 0.89 +3.2 458,789 - -0.4 458,819 + +0.1 458,822 - +0.9 458,822 - +2.2 458,822 - +1.9 458,968 - -1.4 459,077 - prmA Shewana3_0400 0.12 -1.7 459,143 - prmA Shewana3_0400 0.20 +0.6 459,390 - prmA Shewana3_0400 0.48 +0.4 459,390 - prmA Shewana3_0400 0.48 +0.0 459,390 - prmA Shewana3_0400 0.48 -0.8 459,576 + prmA Shewana3_0400 0.69 +0.3 459,666 + prmA Shewana3_0400 0.79 -1.8 459,751 + prmA Shewana3_0400 0.89 -1.1 459,759 - prmA Shewana3_0400 0.90 +0.4 459,759 - prmA Shewana3_0400 0.90 +0.2 459,851 + -1.2 459,858 + +0.7 460,045 + +0.7 460,208 - +0.6 460,344 - +0.7 460,368 + Shewana3_0401 0.10 +0.2 460,387 + Shewana3_0401 0.13 -1.9 460,395 - Shewana3_0401 0.14 +0.3 460,395 - Shewana3_0401 0.14 -1.8 460,402 + Shewana3_0401 0.15 +1.4 460,501 + Shewana3_0401 0.29 -0.9 460,514 - Shewana3_0401 0.30 +0.6 460,514 - Shewana3_0401 0.30 +1.1 460,650 - Shewana3_0401 0.49 -2.4 460,729 - Shewana3_0401 0.60 +0.7 460,799 + Shewana3_0401 0.70 -4.1 460,809 - Shewana3_0401 0.71 +0.5
Or see this region's nucleotide sequence