Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0374 and Shewana3_0375 are separated by 29 nucleotides Shewana3_0375 and Shewana3_R0020 are separated by 208 nucleotides Shewana3_R0020 and Shewana3_R0021 are separated by 53 nucleotides Shewana3_R0021 and Shewana3_R0022 are separated by 14 nucleotides Shewana3_R0022 and Shewana3_R0023 are separated by 53 nucleotides Shewana3_R0023 and Shewana3_0376 are separated by 189 nucleotides
Shewana3_0374: Shewana3_0374 - ATP-dependent DNA helicase Rep (RefSeq), at 411,568 to 413,583
_0374
Shewana3_0375: Shewana3_0375 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 413,613 to 416,243
_0375
Shewana3_R0020: Shewana3_R0020 - tRNA-Arg (RefSeq), at 416,452 to 416,528
_R0020
Shewana3_R0021: Shewana3_R0021 - tRNA-His (RefSeq), at 416,582 to 416,657
_R0021
Shewana3_R0022: Shewana3_R0022 - tRNA-Pro (RefSeq), at 416,672 to 416,748
_R0022
Shewana3_R0023: Shewana3_R0023 - tRNA-Pro (RefSeq), at 416,802 to 416,878
_R0023
Shewana3_0376: Shewana3_0376 - diguanylate cyclase/phosphodiesterase (RefSeq), at 417,068 to 418,987
_0376
Position (kb) 413
414
415
416
417 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 413.616 kb on + strand at 413.789 kb on + strand at 413.944 kb on - strand, within Shewana3_0375 at 414.011 kb on + strand, within Shewana3_0375 at 414.011 kb on - strand, within Shewana3_0375 at 414.024 kb on - strand, within Shewana3_0375 at 414.024 kb on - strand, within Shewana3_0375 at 414.086 kb on + strand, within Shewana3_0375 at 414.163 kb on + strand, within Shewana3_0375 at 414.165 kb on - strand, within Shewana3_0375 at 414.245 kb on - strand, within Shewana3_0375 at 414.261 kb on - strand, within Shewana3_0375 at 414.441 kb on + strand, within Shewana3_0375 at 414.465 kb on - strand, within Shewana3_0375 at 414.549 kb on + strand, within Shewana3_0375 at 414.549 kb on + strand, within Shewana3_0375 at 414.550 kb on - strand, within Shewana3_0375 at 414.550 kb on - strand, within Shewana3_0375 at 414.556 kb on - strand, within Shewana3_0375 at 414.558 kb on - strand, within Shewana3_0375 at 414.618 kb on - strand, within Shewana3_0375 at 414.657 kb on + strand, within Shewana3_0375 at 414.671 kb on - strand, within Shewana3_0375 at 414.775 kb on + strand, within Shewana3_0375 at 414.783 kb on - strand, within Shewana3_0375 at 414.794 kb on + strand, within Shewana3_0375 at 414.802 kb on - strand, within Shewana3_0375 at 414.802 kb on - strand, within Shewana3_0375 at 414.960 kb on + strand, within Shewana3_0375 at 415.009 kb on - strand, within Shewana3_0375 at 415.382 kb on - strand, within Shewana3_0375 at 415.382 kb on - strand, within Shewana3_0375 at 415.422 kb on - strand, within Shewana3_0375 at 415.527 kb on + strand, within Shewana3_0375 at 415.534 kb on + strand, within Shewana3_0375 at 415.534 kb on + strand, within Shewana3_0375 at 415.597 kb on - strand, within Shewana3_0375 at 415.927 kb on + strand, within Shewana3_0375 at 416.057 kb on + strand at 416.148 kb on - strand at 417.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 413,616 + -1.7 413,789 + -1.7 413,944 - Shewana3_0375 0.13 +0.1 414,011 + Shewana3_0375 0.15 +0.4 414,011 - Shewana3_0375 0.15 +0.7 414,024 - Shewana3_0375 0.16 +0.9 414,024 - Shewana3_0375 0.16 -0.8 414,086 + Shewana3_0375 0.18 +0.1 414,163 + Shewana3_0375 0.21 +0.2 414,165 - Shewana3_0375 0.21 +0.6 414,245 - Shewana3_0375 0.24 -0.5 414,261 - Shewana3_0375 0.25 -0.4 414,441 + Shewana3_0375 0.31 -0.2 414,465 - Shewana3_0375 0.32 +0.2 414,549 + Shewana3_0375 0.36 -1.2 414,549 + Shewana3_0375 0.36 +0.6 414,550 - Shewana3_0375 0.36 -1.9 414,550 - Shewana3_0375 0.36 -1.5 414,556 - Shewana3_0375 0.36 -1.7 414,558 - Shewana3_0375 0.36 +1.2 414,618 - Shewana3_0375 0.38 -0.1 414,657 + Shewana3_0375 0.40 -1.7 414,671 - Shewana3_0375 0.40 -1.5 414,775 + Shewana3_0375 0.44 -0.1 414,783 - Shewana3_0375 0.44 +1.4 414,794 + Shewana3_0375 0.45 -3.3 414,802 - Shewana3_0375 0.45 -2.8 414,802 - Shewana3_0375 0.45 -1.6 414,960 + Shewana3_0375 0.51 +0.7 415,009 - Shewana3_0375 0.53 +0.4 415,382 - Shewana3_0375 0.67 -2.3 415,382 - Shewana3_0375 0.67 +0.5 415,422 - Shewana3_0375 0.69 +0.4 415,527 + Shewana3_0375 0.73 +0.7 415,534 + Shewana3_0375 0.73 +0.5 415,534 + Shewana3_0375 0.73 -2.4 415,597 - Shewana3_0375 0.75 -0.5 415,927 + Shewana3_0375 0.88 -1.0 416,057 + +0.5 416,148 - -1.6 417,114 + -1.8
Or see this region's nucleotide sequence