Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0365 and Shewana3_0366 are separated by 162 nucleotides Shewana3_0366 and Shewana3_0367 are separated by 236 nucleotides
Shewana3_0365: Shewana3_0365 - hypothetical protein (RefSeq), at 401,643 to 403,118
_0365
Shewana3_0366: Shewana3_0366 - hypothetical protein (RefSeq), at 403,281 to 403,808
_0366
Shewana3_0367: Shewana3_0367 - hypothetical protein (RefSeq), at 404,045 to 405,211
_0367
Position (kb) 403
404 Strain fitness (log2 ratio) -2
-1
0
1 at 402.316 kb on - strand, within Shewana3_0365 at 402.377 kb on + strand, within Shewana3_0365 at 402.598 kb on + strand, within Shewana3_0365 at 402.777 kb on + strand, within Shewana3_0365 at 402.925 kb on + strand, within Shewana3_0365 at 402.933 kb on + strand, within Shewana3_0365 at 402.979 kb on + strand at 403.089 kb on + strand at 403.359 kb on - strand, within Shewana3_0366 at 403.394 kb on + strand, within Shewana3_0366 at 403.541 kb on + strand, within Shewana3_0366 at 403.572 kb on - strand, within Shewana3_0366 at 403.683 kb on + strand, within Shewana3_0366 at 403.717 kb on + strand, within Shewana3_0366 at 403.842 kb on - strand at 403.875 kb on + strand at 403.877 kb on - strand at 404.107 kb on - strand at 404.108 kb on - strand at 404.249 kb on - strand, within Shewana3_0367 at 404.385 kb on - strand, within Shewana3_0367 at 404.714 kb on - strand, within Shewana3_0367
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 402,316 - Shewana3_0365 0.46 +0.3 402,377 + Shewana3_0365 0.50 +0.4 402,598 + Shewana3_0365 0.65 +0.4 402,777 + Shewana3_0365 0.77 +0.2 402,925 + Shewana3_0365 0.87 -1.7 402,933 + Shewana3_0365 0.87 +0.3 402,979 + +0.4 403,089 + -1.1 403,359 - Shewana3_0366 0.15 -1.5 403,394 + Shewana3_0366 0.21 -0.8 403,541 + Shewana3_0366 0.49 +0.3 403,572 - Shewana3_0366 0.55 -1.9 403,683 + Shewana3_0366 0.76 -1.2 403,717 + Shewana3_0366 0.83 -0.9 403,842 - +0.5 403,875 + +0.3 403,877 - -1.6 404,107 - +0.7 404,108 - +0.4 404,249 - Shewana3_0367 0.17 +1.2 404,385 - Shewana3_0367 0.29 +0.1 404,714 - Shewana3_0367 0.57 -1.5
Or see this region's nucleotide sequence