Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0362 and Shewana3_0363 are separated by 267 nucleotides Shewana3_0363 and Shewana3_0364 are separated by 102 nucleotides Shewana3_0364 and Shewana3_0365 are separated by 92 nucleotides
Shewana3_0362: Shewana3_0362 - hypothetical protein (RefSeq), at 399,616 to 399,855
_0362
Shewana3_0363: Shewana3_0363 - hypothetical protein (RefSeq), at 400,123 to 400,650
_0363
Shewana3_0364: Shewana3_0364 - phosphatidylglycerophosphatase (RefSeq), at 400,753 to 401,550
_0364
Shewana3_0365: Shewana3_0365 - hypothetical protein (RefSeq), at 401,643 to 403,118
_0365
Position (kb) 400
401
402 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 399.850 kb on + strand at 400.289 kb on + strand, within Shewana3_0363 at 400.411 kb on + strand, within Shewana3_0363 at 400.416 kb on + strand, within Shewana3_0363 at 400.416 kb on + strand, within Shewana3_0363 at 400.416 kb on + strand, within Shewana3_0363 at 400.494 kb on + strand, within Shewana3_0363 at 400.731 kb on - strand at 400.855 kb on - strand, within Shewana3_0364 at 400.971 kb on + strand, within Shewana3_0364 at 400.971 kb on + strand, within Shewana3_0364 at 401.116 kb on - strand, within Shewana3_0364 at 401.123 kb on + strand, within Shewana3_0364 at 401.354 kb on + strand, within Shewana3_0364 at 401.394 kb on - strand, within Shewana3_0364 at 401.520 kb on - strand at 401.521 kb on + strand at 401.553 kb on + strand at 401.566 kb on + strand at 401.643 kb on + strand at 401.643 kb on + strand at 401.643 kb on + strand at 401.645 kb on + strand at 401.663 kb on - strand at 401.721 kb on + strand at 401.760 kb on - strand at 401.760 kb on - strand at 401.928 kb on + strand, within Shewana3_0365 at 401.958 kb on + strand, within Shewana3_0365 at 401.966 kb on - strand, within Shewana3_0365 at 401.966 kb on - strand, within Shewana3_0365 at 401.978 kb on - strand, within Shewana3_0365 at 402.090 kb on - strand, within Shewana3_0365 at 402.090 kb on - strand, within Shewana3_0365 at 402.090 kb on - strand, within Shewana3_0365 at 402.107 kb on + strand, within Shewana3_0365 at 402.220 kb on + strand, within Shewana3_0365 at 402.228 kb on - strand, within Shewana3_0365 at 402.316 kb on - strand, within Shewana3_0365 at 402.377 kb on + strand, within Shewana3_0365
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 399,850 + -1.2 400,289 + Shewana3_0363 0.31 +0.8 400,411 + Shewana3_0363 0.55 +0.4 400,416 + Shewana3_0363 0.55 +0.4 400,416 + Shewana3_0363 0.55 +0.1 400,416 + Shewana3_0363 0.55 -4.9 400,494 + Shewana3_0363 0.70 -2.0 400,731 - -1.2 400,855 - Shewana3_0364 0.13 -1.0 400,971 + Shewana3_0364 0.27 -0.4 400,971 + Shewana3_0364 0.27 +1.1 401,116 - Shewana3_0364 0.45 +0.1 401,123 + Shewana3_0364 0.46 +0.2 401,354 + Shewana3_0364 0.75 +1.0 401,394 - Shewana3_0364 0.80 +1.1 401,520 - -0.5 401,521 + -1.3 401,553 + +0.2 401,566 + +0.7 401,643 + +0.2 401,643 + +0.6 401,643 + +1.1 401,645 + -1.5 401,663 - +0.0 401,721 + -0.3 401,760 - -0.0 401,760 - -2.5 401,928 + Shewana3_0365 0.19 -0.2 401,958 + Shewana3_0365 0.21 -0.2 401,966 - Shewana3_0365 0.22 +0.9 401,966 - Shewana3_0365 0.22 +0.3 401,978 - Shewana3_0365 0.23 +0.3 402,090 - Shewana3_0365 0.30 +0.7 402,090 - Shewana3_0365 0.30 +0.1 402,090 - Shewana3_0365 0.30 -2.0 402,107 + Shewana3_0365 0.31 +2.2 402,220 + Shewana3_0365 0.39 -1.9 402,228 - Shewana3_0365 0.40 -1.1 402,316 - Shewana3_0365 0.46 +0.3 402,377 + Shewana3_0365 0.50 +0.4
Or see this region's nucleotide sequence