Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0350 and Shewana3_0351 are separated by 151 nucleotides Shewana3_0351 and Shewana3_0352 overlap by 38 nucleotides Shewana3_0352 and Shewana3_0353 are separated by 172 nucleotides
Shewana3_0350: Shewana3_0350 - diguanylate cyclase (RefSeq), at 384,315 to 386,132
_0350
Shewana3_0351: Shewana3_0351 - hypothetical protein (RefSeq), at 386,284 to 386,697
_0351
Shewana3_0352: Shewana3_0352 - amino acid ABC transporter periplasmic protein (RefSeq), at 386,660 to 387,499
_0352
Shewana3_0353: Shewana3_0353 - hypothetical protein (RefSeq), at 387,672 to 388,988
_0353
Position (kb) 386
387 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 385.334 kb on - strand, within Shewana3_0350 at 385.401 kb on + strand, within Shewana3_0350 at 385.546 kb on + strand, within Shewana3_0350 at 385.554 kb on - strand, within Shewana3_0350 at 385.647 kb on - strand, within Shewana3_0350 at 385.775 kb on + strand, within Shewana3_0350 at 385.783 kb on - strand, within Shewana3_0350 at 385.831 kb on - strand, within Shewana3_0350 at 385.831 kb on - strand, within Shewana3_0350 at 385.841 kb on + strand, within Shewana3_0350 at 385.895 kb on + strand, within Shewana3_0350 at 385.903 kb on - strand, within Shewana3_0350 at 385.924 kb on - strand, within Shewana3_0350 at 386.041 kb on + strand at 386.050 kb on + strand at 386.050 kb on + strand at 386.059 kb on - strand at 386.113 kb on - strand at 386.118 kb on - strand at 386.276 kb on + strand at 386.284 kb on - strand at 386.368 kb on + strand, within Shewana3_0351 at 386.373 kb on + strand, within Shewana3_0351 at 386.436 kb on - strand, within Shewana3_0351 at 386.624 kb on + strand, within Shewana3_0351 at 386.624 kb on + strand, within Shewana3_0351 at 386.759 kb on - strand, within Shewana3_0352 at 386.830 kb on + strand, within Shewana3_0352 at 386.887 kb on + strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 387.045 kb on + strand, within Shewana3_0352 at 387.169 kb on - strand, within Shewana3_0352 at 387.242 kb on + strand, within Shewana3_0352 at 387.260 kb on - strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.294 kb on - strand, within Shewana3_0352 at 387.294 kb on - strand, within Shewana3_0352 at 387.408 kb on - strand, within Shewana3_0352 at 387.427 kb on - strand at 387.467 kb on - strand at 387.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 385,334 - Shewana3_0350 0.56 -1.6 385,401 + Shewana3_0350 0.60 +0.2 385,546 + Shewana3_0350 0.68 +0.2 385,554 - Shewana3_0350 0.68 +0.6 385,647 - Shewana3_0350 0.73 +0.6 385,775 + Shewana3_0350 0.80 +0.4 385,783 - Shewana3_0350 0.81 -1.0 385,831 - Shewana3_0350 0.83 -3.7 385,831 - Shewana3_0350 0.83 -1.5 385,841 + Shewana3_0350 0.84 +0.1 385,895 + Shewana3_0350 0.87 +1.0 385,903 - Shewana3_0350 0.87 -2.2 385,924 - Shewana3_0350 0.89 -1.8 386,041 + -3.7 386,050 + +0.4 386,050 + -1.6 386,059 - -1.7 386,113 - +0.1 386,118 - -1.6 386,276 + -0.8 386,284 - +0.6 386,368 + Shewana3_0351 0.20 -2.2 386,373 + Shewana3_0351 0.21 -1.6 386,436 - Shewana3_0351 0.37 -2.1 386,624 + Shewana3_0351 0.82 +0.5 386,624 + Shewana3_0351 0.82 -1.9 386,759 - Shewana3_0352 0.12 +0.7 386,830 + Shewana3_0352 0.20 -0.2 386,887 + Shewana3_0352 0.27 -0.4 386,895 - Shewana3_0352 0.28 -0.9 386,895 - Shewana3_0352 0.28 +0.6 386,895 - Shewana3_0352 0.28 +0.8 387,045 + Shewana3_0352 0.46 +0.6 387,169 - Shewana3_0352 0.61 -1.4 387,242 + Shewana3_0352 0.69 +0.3 387,260 - Shewana3_0352 0.71 -1.5 387,286 + Shewana3_0352 0.75 +0.2 387,286 + Shewana3_0352 0.75 -1.4 387,286 + Shewana3_0352 0.75 -1.4 387,286 + Shewana3_0352 0.75 -2.2 387,294 - Shewana3_0352 0.75 -0.1 387,294 - Shewana3_0352 0.75 -0.4 387,408 - Shewana3_0352 0.89 +0.7 387,427 - +0.9 387,467 - +1.0 387,472 - +0.8
Or see this region's nucleotide sequence