Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0308 and Shewana3_0309 are separated by 186 nucleotides Shewana3_0309 and Shewana3_0310 are separated by 29 nucleotides Shewana3_0310 and rbn are separated by 81 nucleotides
Shewana3_0308: Shewana3_0308 - diguanylate cyclase (RefSeq), at 346,356 to 348,101
_0308
Shewana3_0309: Shewana3_0309 - hypothetical protein (RefSeq), at 348,288 to 348,941
_0309
Shewana3_0310: Shewana3_0310 - hypothetical protein (RefSeq), at 348,971 to 349,192
_0310
Shewana3_0311: rbn - ribonuclease BN (RefSeq), at 349,274 to 350,287
rbn
Position (kb) 348
349 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 347.387 kb on + strand, within Shewana3_0308 at 347.394 kb on + strand, within Shewana3_0308 at 347.395 kb on - strand, within Shewana3_0308 at 347.399 kb on + strand, within Shewana3_0308 at 347.412 kb on - strand, within Shewana3_0308 at 347.746 kb on + strand, within Shewana3_0308 at 347.754 kb on - strand, within Shewana3_0308 at 347.779 kb on + strand, within Shewana3_0308 at 347.795 kb on + strand, within Shewana3_0308 at 348.261 kb on - strand at 348.293 kb on - strand at 348.505 kb on + strand, within Shewana3_0309 at 348.505 kb on + strand, within Shewana3_0309 at 348.506 kb on - strand, within Shewana3_0309 at 348.741 kb on - strand, within Shewana3_0309 at 348.746 kb on + strand, within Shewana3_0309 at 348.917 kb on + strand at 348.925 kb on - strand at 348.925 kb on - strand at 348.925 kb on - strand at 348.986 kb on - strand at 349.005 kb on + strand, within Shewana3_0310 at 349.057 kb on + strand, within Shewana3_0310 at 349.057 kb on + strand, within Shewana3_0310 at 349.210 kb on - strand at 349.330 kb on - strand at 349.406 kb on + strand, within rbn at 349.406 kb on + strand, within rbn at 349.414 kb on - strand, within rbn at 349.427 kb on - strand, within rbn at 349.543 kb on - strand, within rbn at 349.543 kb on - strand, within rbn at 349.781 kb on - strand, within rbn at 349.867 kb on + strand, within rbn
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 347,387 + Shewana3_0308 0.59 +1.3 347,394 + Shewana3_0308 0.59 +0.6 347,395 - Shewana3_0308 0.60 -1.1 347,399 + Shewana3_0308 0.60 +0.7 347,412 - Shewana3_0308 0.60 +0.6 347,746 + Shewana3_0308 0.80 +0.8 347,754 - Shewana3_0308 0.80 +0.3 347,779 + Shewana3_0308 0.82 +1.0 347,795 + Shewana3_0308 0.82 +0.1 348,261 - +0.2 348,293 - +0.6 348,505 + Shewana3_0309 0.33 +0.6 348,505 + Shewana3_0309 0.33 -0.4 348,506 - Shewana3_0309 0.33 +1.4 348,741 - Shewana3_0309 0.69 -0.2 348,746 + Shewana3_0309 0.70 +0.8 348,917 + +0.3 348,925 - +1.3 348,925 - -1.0 348,925 - +0.6 348,986 - +1.5 349,005 + Shewana3_0310 0.15 +0.7 349,057 + Shewana3_0310 0.39 -1.6 349,057 + Shewana3_0310 0.39 +0.3 349,210 - +0.1 349,330 - -2.4 349,406 + rbn Shewana3_0311 0.13 -1.0 349,406 + rbn Shewana3_0311 0.13 -0.1 349,414 - rbn Shewana3_0311 0.14 -2.0 349,427 - rbn Shewana3_0311 0.15 -0.9 349,543 - rbn Shewana3_0311 0.27 -2.6 349,543 - rbn Shewana3_0311 0.27 -0.7 349,781 - rbn Shewana3_0311 0.50 -1.9 349,867 + rbn Shewana3_0311 0.58 -3.2
Or see this region's nucleotide sequence