Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0307 and Shewana3_0308 are separated by 174 nucleotides Shewana3_0308 and Shewana3_0309 are separated by 186 nucleotides Shewana3_0309 and Shewana3_0310 are separated by 29 nucleotides
Shewana3_0307: Shewana3_0307 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 344,934 to 346,181
_0307
Shewana3_0308: Shewana3_0308 - diguanylate cyclase (RefSeq), at 346,356 to 348,101
_0308
Shewana3_0309: Shewana3_0309 - hypothetical protein (RefSeq), at 348,288 to 348,941
_0309
Shewana3_0310: Shewana3_0310 - hypothetical protein (RefSeq), at 348,971 to 349,192
_0310
Position (kb) 346
347
348
349 Strain fitness (log2 ratio) -2
-1
0
1 at 345.497 kb on - strand, within Shewana3_0307 at 345.684 kb on - strand, within Shewana3_0307 at 345.797 kb on + strand, within Shewana3_0307 at 345.819 kb on - strand, within Shewana3_0307 at 345.852 kb on - strand, within Shewana3_0307 at 345.855 kb on + strand, within Shewana3_0307 at 345.855 kb on + strand, within Shewana3_0307 at 345.863 kb on - strand, within Shewana3_0307 at 346.053 kb on + strand, within Shewana3_0307 at 346.061 kb on - strand at 346.182 kb on - strand at 346.298 kb on + strand at 346.307 kb on - strand at 346.434 kb on + strand at 346.466 kb on + strand at 346.467 kb on + strand at 346.474 kb on - strand at 346.507 kb on - strand at 346.507 kb on - strand at 346.548 kb on + strand, within Shewana3_0308 at 346.679 kb on + strand, within Shewana3_0308 at 346.765 kb on - strand, within Shewana3_0308 at 347.387 kb on + strand, within Shewana3_0308 at 347.394 kb on + strand, within Shewana3_0308 at 347.395 kb on - strand, within Shewana3_0308 at 347.399 kb on + strand, within Shewana3_0308 at 347.412 kb on - strand, within Shewana3_0308 at 347.746 kb on + strand, within Shewana3_0308 at 347.754 kb on - strand, within Shewana3_0308 at 347.779 kb on + strand, within Shewana3_0308 at 347.795 kb on + strand, within Shewana3_0308 at 348.261 kb on - strand at 348.293 kb on - strand at 348.505 kb on + strand, within Shewana3_0309 at 348.505 kb on + strand, within Shewana3_0309 at 348.506 kb on - strand, within Shewana3_0309 at 348.741 kb on - strand, within Shewana3_0309 at 348.746 kb on + strand, within Shewana3_0309 at 348.917 kb on + strand at 348.925 kb on - strand at 348.925 kb on - strand at 348.925 kb on - strand at 348.986 kb on - strand at 349.005 kb on + strand, within Shewana3_0310 at 349.057 kb on + strand, within Shewana3_0310 at 349.057 kb on + strand, within Shewana3_0310
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 345,497 - Shewana3_0307 0.45 +0.8 345,684 - Shewana3_0307 0.60 -0.0 345,797 + Shewana3_0307 0.69 -1.5 345,819 - Shewana3_0307 0.71 +0.3 345,852 - Shewana3_0307 0.74 +0.3 345,855 + Shewana3_0307 0.74 -0.2 345,855 + Shewana3_0307 0.74 +0.7 345,863 - Shewana3_0307 0.74 +0.9 346,053 + Shewana3_0307 0.90 +1.4 346,061 - -1.3 346,182 - -2.0 346,298 + +1.0 346,307 - +1.3 346,434 + +0.5 346,466 + -1.4 346,467 + +1.1 346,474 - -0.4 346,507 - +0.4 346,507 - +0.4 346,548 + Shewana3_0308 0.11 +0.4 346,679 + Shewana3_0308 0.18 -1.4 346,765 - Shewana3_0308 0.23 +0.0 347,387 + Shewana3_0308 0.59 +1.3 347,394 + Shewana3_0308 0.59 +0.6 347,395 - Shewana3_0308 0.60 -1.1 347,399 + Shewana3_0308 0.60 +0.7 347,412 - Shewana3_0308 0.60 +0.6 347,746 + Shewana3_0308 0.80 +0.8 347,754 - Shewana3_0308 0.80 +0.3 347,779 + Shewana3_0308 0.82 +1.0 347,795 + Shewana3_0308 0.82 +0.1 348,261 - +0.2 348,293 - +0.6 348,505 + Shewana3_0309 0.33 +0.6 348,505 + Shewana3_0309 0.33 -0.4 348,506 - Shewana3_0309 0.33 +1.4 348,741 - Shewana3_0309 0.69 -0.2 348,746 + Shewana3_0309 0.70 +0.8 348,917 + +0.3 348,925 - +1.3 348,925 - -1.0 348,925 - +0.6 348,986 - +1.5 349,005 + Shewana3_0310 0.15 +0.7 349,057 + Shewana3_0310 0.39 -1.6 349,057 + Shewana3_0310 0.39 +0.3
Or see this region's nucleotide sequence