Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0302 and Shewana3_0303 are separated by 138 nucleotides Shewana3_0303 and Shewana3_0304 are separated by 44 nucleotides Shewana3_0304 and Shewana3_0305 are separated by 10 nucleotides
Shewana3_0302: Shewana3_0302 - AraC family transcriptional regulator (RefSeq), at 338,762 to 339,769
_0302
Shewana3_0303: Shewana3_0303 - short-chain dehydrogenase/reductase SDR (RefSeq), at 339,908 to 340,669
_0303
Shewana3_0304: Shewana3_0304 - hypothetical protein (RefSeq), at 340,714 to 341,220
_0304
Shewana3_0305: Shewana3_0305 - alcohol dehydrogenase (RefSeq), at 341,231 to 342,307
_0305
Position (kb) 340
341
342 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 339.900 kb on + strand at 339.906 kb on - strand at 339.913 kb on - strand at 339.919 kb on + strand at 340.102 kb on + strand, within Shewana3_0303 at 340.297 kb on - strand, within Shewana3_0303 at 340.297 kb on - strand, within Shewana3_0303 at 340.352 kb on - strand, within Shewana3_0303 at 340.424 kb on - strand, within Shewana3_0303 at 340.470 kb on + strand, within Shewana3_0303 at 340.470 kb on + strand, within Shewana3_0303 at 340.470 kb on + strand, within Shewana3_0303 at 340.470 kb on + strand, within Shewana3_0303 at 340.472 kb on + strand, within Shewana3_0303 at 340.485 kb on + strand, within Shewana3_0303 at 340.523 kb on + strand, within Shewana3_0303 at 340.634 kb on - strand at 340.680 kb on + strand at 340.952 kb on + strand, within Shewana3_0304 at 341.016 kb on + strand, within Shewana3_0304 at 341.128 kb on + strand, within Shewana3_0304 at 341.418 kb on + strand, within Shewana3_0305 at 341.476 kb on + strand, within Shewana3_0305 at 341.477 kb on - strand, within Shewana3_0305 at 341.555 kb on + strand, within Shewana3_0305 at 341.563 kb on - strand, within Shewana3_0305 at 341.594 kb on - strand, within Shewana3_0305 at 341.605 kb on - strand, within Shewana3_0305 at 341.652 kb on - strand, within Shewana3_0305 at 341.708 kb on + strand, within Shewana3_0305 at 341.895 kb on + strand, within Shewana3_0305 at 341.951 kb on - strand, within Shewana3_0305
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 339,900 + -1.3 339,906 - +1.1 339,913 - +0.6 339,919 + -0.4 340,102 + Shewana3_0303 0.25 -2.2 340,297 - Shewana3_0303 0.51 -1.5 340,297 - Shewana3_0303 0.51 +0.5 340,352 - Shewana3_0303 0.58 -1.0 340,424 - Shewana3_0303 0.68 +0.8 340,470 + Shewana3_0303 0.74 -1.6 340,470 + Shewana3_0303 0.74 +0.4 340,470 + Shewana3_0303 0.74 +0.3 340,470 + Shewana3_0303 0.74 +0.3 340,472 + Shewana3_0303 0.74 +0.6 340,485 + Shewana3_0303 0.76 +0.5 340,523 + Shewana3_0303 0.81 +0.2 340,634 - +0.5 340,680 + +1.0 340,952 + Shewana3_0304 0.47 -1.8 341,016 + Shewana3_0304 0.60 -2.9 341,128 + Shewana3_0304 0.82 +0.6 341,418 + Shewana3_0305 0.17 +0.5 341,476 + Shewana3_0305 0.23 -0.7 341,477 - Shewana3_0305 0.23 -1.8 341,555 + Shewana3_0305 0.30 -1.0 341,563 - Shewana3_0305 0.31 -2.6 341,594 - Shewana3_0305 0.34 +0.2 341,605 - Shewana3_0305 0.35 +0.4 341,652 - Shewana3_0305 0.39 +0.4 341,708 + Shewana3_0305 0.44 -1.8 341,895 + Shewana3_0305 0.62 -0.4 341,951 - Shewana3_0305 0.67 +0.8
Or see this region's nucleotide sequence