Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0295 and Shewana3_0296 are separated by 135 nucleotides Shewana3_0296 and Shewana3_0297 are separated by 184 nucleotides Shewana3_0297 and Shewana3_0298 are separated by 389 nucleotides Shewana3_0298 and Shewana3_0299 are separated by 59 nucleotides
Shewana3_0295: Shewana3_0295 - aminotransferase, class V (RefSeq), at 328,100 to 329,221
_0295
Shewana3_0296: Shewana3_0296 - hypothetical protein (RefSeq), at 329,357 to 329,680
_0296
Shewana3_0297: Shewana3_0297 - sodium:dicarboxylate symporter (RefSeq), at 329,865 to 331,109
_0297
Shewana3_0298: Shewana3_0298 - hypothetical protein (RefSeq), at 331,499 to 331,765
_0298
Shewana3_0299: Shewana3_0299 - TonB-dependent siderophore receptor (RefSeq), at 331,825 to 334,062
_0299
Position (kb) 329
330
331
332 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 328.892 kb on - strand, within Shewana3_0295 at 328.928 kb on + strand, within Shewana3_0295 at 329.136 kb on + strand at 329.172 kb on - strand at 329.200 kb on + strand at 329.241 kb on - strand at 329.404 kb on + strand, within Shewana3_0296 at 329.404 kb on + strand, within Shewana3_0296 at 329.404 kb on + strand, within Shewana3_0296 at 329.665 kb on + strand at 329.670 kb on + strand at 329.724 kb on - strand at 329.787 kb on + strand at 329.884 kb on + strand at 330.003 kb on + strand, within Shewana3_0297 at 330.003 kb on + strand, within Shewana3_0297 at 330.003 kb on + strand, within Shewana3_0297 at 330.003 kb on + strand, within Shewana3_0297 at 330.011 kb on - strand, within Shewana3_0297 at 330.043 kb on - strand, within Shewana3_0297 at 330.071 kb on - strand, within Shewana3_0297 at 330.077 kb on + strand, within Shewana3_0297 at 330.077 kb on + strand, within Shewana3_0297 at 330.085 kb on - strand, within Shewana3_0297 at 330.085 kb on - strand, within Shewana3_0297 at 330.134 kb on - strand, within Shewana3_0297 at 330.161 kb on - strand, within Shewana3_0297 at 330.262 kb on - strand, within Shewana3_0297 at 330.566 kb on - strand, within Shewana3_0297 at 330.700 kb on + strand, within Shewana3_0297 at 330.753 kb on + strand, within Shewana3_0297 at 330.763 kb on + strand, within Shewana3_0297 at 330.945 kb on + strand, within Shewana3_0297 at 330.971 kb on + strand, within Shewana3_0297 at 331.113 kb on - strand at 331.144 kb on + strand at 331.157 kb on - strand at 331.162 kb on - strand at 331.252 kb on - strand at 331.325 kb on + strand at 331.330 kb on + strand at 331.357 kb on - strand at 331.363 kb on + strand at 331.611 kb on + strand, within Shewana3_0298 at 331.619 kb on - strand, within Shewana3_0298 at 331.821 kb on + strand at 331.829 kb on - strand at 332.106 kb on - strand, within Shewana3_0299
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 328,892 - Shewana3_0295 0.71 +0.6 328,928 + Shewana3_0295 0.74 -1.3 329,136 + -4.1 329,172 - +0.4 329,200 + -1.4 329,241 - +1.3 329,404 + Shewana3_0296 0.15 +0.6 329,404 + Shewana3_0296 0.15 +1.3 329,404 + Shewana3_0296 0.15 -1.4 329,665 + +0.4 329,670 + -2.1 329,724 - +0.5 329,787 + -1.7 329,884 + -0.3 330,003 + Shewana3_0297 0.11 -1.8 330,003 + Shewana3_0297 0.11 +0.3 330,003 + Shewana3_0297 0.11 +0.3 330,003 + Shewana3_0297 0.11 -0.1 330,011 - Shewana3_0297 0.12 -1.2 330,043 - Shewana3_0297 0.14 +0.1 330,071 - Shewana3_0297 0.17 -1.7 330,077 + Shewana3_0297 0.17 +0.5 330,077 + Shewana3_0297 0.17 -1.4 330,085 - Shewana3_0297 0.18 +0.5 330,085 - Shewana3_0297 0.18 -1.7 330,134 - Shewana3_0297 0.22 +0.3 330,161 - Shewana3_0297 0.24 +0.3 330,262 - Shewana3_0297 0.32 -1.4 330,566 - Shewana3_0297 0.56 -1.6 330,700 + Shewana3_0297 0.67 -0.6 330,753 + Shewana3_0297 0.71 +0.2 330,763 + Shewana3_0297 0.72 +0.3 330,945 + Shewana3_0297 0.87 -3.4 330,971 + Shewana3_0297 0.89 +1.2 331,113 - +0.3 331,144 + -1.6 331,157 - -1.0 331,162 - -0.2 331,252 - +0.4 331,325 + -2.5 331,330 + +0.6 331,357 - -1.6 331,363 + +0.3 331,611 + Shewana3_0298 0.42 -1.7 331,619 - Shewana3_0298 0.45 +0.5 331,821 + -0.8 331,829 - -0.5 332,106 - Shewana3_0299 0.13 +0.5
Or see this region's nucleotide sequence