Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0248 and Shewana3_0249 are separated by 135 nucleotides Shewana3_0249 and Shewana3_0250 are separated by 69 nucleotides
Shewana3_0248: Shewana3_0248 - LysR family transcriptional regulator (RefSeq), at 278,282 to 279,199
_0248
Shewana3_0249: Shewana3_0249 - short-chain dehydrogenase/reductase SDR (RefSeq), at 279,335 to 280,096
_0249
Shewana3_0250: Shewana3_0250 - aldehyde dehydrogenase (RefSeq), at 280,166 to 281,686
_0250
Position (kb) 279
280
281 Strain fitness (log2 ratio) -2
-1
0
1 at 278.386 kb on + strand, within Shewana3_0248 at 278.485 kb on - strand, within Shewana3_0248 at 278.543 kb on + strand, within Shewana3_0248 at 278.551 kb on - strand, within Shewana3_0248 at 278.686 kb on + strand, within Shewana3_0248 at 278.694 kb on - strand, within Shewana3_0248 at 278.717 kb on - strand, within Shewana3_0248 at 278.822 kb on - strand, within Shewana3_0248 at 278.888 kb on - strand, within Shewana3_0248 at 279.115 kb on + strand at 279.400 kb on + strand at 279.500 kb on + strand, within Shewana3_0249 at 279.647 kb on + strand, within Shewana3_0249 at 279.665 kb on - strand, within Shewana3_0249 at 279.778 kb on - strand, within Shewana3_0249 at 279.977 kb on + strand, within Shewana3_0249 at 279.977 kb on + strand, within Shewana3_0249 at 280.004 kb on + strand, within Shewana3_0249 at 280.307 kb on - strand at 280.375 kb on - strand, within Shewana3_0250 at 280.522 kb on + strand, within Shewana3_0250 at 280.557 kb on + strand, within Shewana3_0250 at 280.993 kb on + strand, within Shewana3_0250
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 278,386 + Shewana3_0248 0.11 +0.7 278,485 - Shewana3_0248 0.22 +0.4 278,543 + Shewana3_0248 0.28 +0.4 278,551 - Shewana3_0248 0.29 +0.9 278,686 + Shewana3_0248 0.44 -2.5 278,694 - Shewana3_0248 0.45 -1.4 278,717 - Shewana3_0248 0.47 +0.4 278,822 - Shewana3_0248 0.59 -1.6 278,888 - Shewana3_0248 0.66 +0.5 279,115 + +0.5 279,400 + +0.6 279,500 + Shewana3_0249 0.22 -1.6 279,647 + Shewana3_0249 0.41 +0.3 279,665 - Shewana3_0249 0.43 +0.2 279,778 - Shewana3_0249 0.58 -0.2 279,977 + Shewana3_0249 0.84 -1.6 279,977 + Shewana3_0249 0.84 -2.1 280,004 + Shewana3_0249 0.88 -1.7 280,307 - +0.4 280,375 - Shewana3_0250 0.14 -0.7 280,522 + Shewana3_0250 0.23 -0.0 280,557 + Shewana3_0250 0.26 +0.5 280,993 + Shewana3_0250 0.54 -2.0
Or see this region's nucleotide sequence