Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0161 and Shewana3_0162 are separated by 63 nucleotides Shewana3_0162 and Shewana3_0163 are separated by 105 nucleotides Shewana3_0163 and Shewana3_0164 overlap by 23 nucleotides
Shewana3_0161: Shewana3_0161 - type II secretion system protein N (RefSeq), at 184,805 to 185,566
_0161
Shewana3_0162: Shewana3_0162 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 185,630 to 186,292
_0162
Shewana3_0163: Shewana3_0163 - aspartyl/asparaginyl beta-hydroxylase (RefSeq), at 186,398 to 187,012
_0163
Shewana3_0164: Shewana3_0164 - phytanoyl-CoA dioxygenase (RefSeq), at 186,990 to 189,002
_0164
Position (kb) 186
187
188 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 185.490 kb on - strand at 185.529 kb on + strand at 185.890 kb on + strand, within Shewana3_0162 at 185.923 kb on + strand, within Shewana3_0162 at 185.937 kb on + strand, within Shewana3_0162 at 185.937 kb on + strand, within Shewana3_0162 at 185.945 kb on - strand, within Shewana3_0162 at 186.049 kb on - strand, within Shewana3_0162 at 186.122 kb on - strand, within Shewana3_0162 at 186.171 kb on - strand, within Shewana3_0162 at 186.171 kb on - strand, within Shewana3_0162 at 186.270 kb on - strand at 186.406 kb on + strand at 186.552 kb on - strand, within Shewana3_0163 at 186.563 kb on + strand, within Shewana3_0163 at 186.571 kb on - strand, within Shewana3_0163 at 186.578 kb on + strand, within Shewana3_0163 at 186.654 kb on + strand, within Shewana3_0163 at 186.764 kb on + strand, within Shewana3_0163 at 186.772 kb on - strand, within Shewana3_0163 at 186.777 kb on + strand, within Shewana3_0163 at 186.777 kb on + strand, within Shewana3_0163 at 186.785 kb on - strand, within Shewana3_0163 at 186.847 kb on + strand, within Shewana3_0163 at 187.012 kb on + strand at 187.047 kb on - strand at 187.078 kb on + strand at 187.078 kb on + strand at 187.180 kb on + strand at 187.250 kb on - strand, within Shewana3_0164 at 187.294 kb on - strand, within Shewana3_0164 at 187.428 kb on + strand, within Shewana3_0164 at 187.436 kb on - strand, within Shewana3_0164 at 187.436 kb on - strand, within Shewana3_0164 at 187.490 kb on + strand, within Shewana3_0164 at 187.527 kb on + strand, within Shewana3_0164 at 187.925 kb on - strand, within Shewana3_0164
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 185,490 - +2.1 185,529 + -1.5 185,890 + Shewana3_0162 0.39 -0.9 185,923 + Shewana3_0162 0.44 +0.3 185,937 + Shewana3_0162 0.46 +0.5 185,937 + Shewana3_0162 0.46 +0.3 185,945 - Shewana3_0162 0.48 -1.5 186,049 - Shewana3_0162 0.63 -1.0 186,122 - Shewana3_0162 0.74 +0.5 186,171 - Shewana3_0162 0.82 +0.5 186,171 - Shewana3_0162 0.82 -1.6 186,270 - +0.8 186,406 + -1.0 186,552 - Shewana3_0163 0.25 -0.7 186,563 + Shewana3_0163 0.27 -1.3 186,571 - Shewana3_0163 0.28 -1.8 186,578 + Shewana3_0163 0.29 +0.2 186,654 + Shewana3_0163 0.42 +1.3 186,764 + Shewana3_0163 0.60 +0.5 186,772 - Shewana3_0163 0.61 +0.3 186,777 + Shewana3_0163 0.62 +0.3 186,777 + Shewana3_0163 0.62 +0.3 186,785 - Shewana3_0163 0.63 +0.0 186,847 + Shewana3_0163 0.73 -1.2 187,012 + -1.7 187,047 - -3.0 187,078 + +0.3 187,078 + +0.4 187,180 + -0.3 187,250 - Shewana3_0164 0.13 +0.3 187,294 - Shewana3_0164 0.15 -0.0 187,428 + Shewana3_0164 0.22 +0.5 187,436 - Shewana3_0164 0.22 +1.3 187,436 - Shewana3_0164 0.22 +0.2 187,490 + Shewana3_0164 0.25 +0.2 187,527 + Shewana3_0164 0.27 -2.5 187,925 - Shewana3_0164 0.46 -1.3
Or see this region's nucleotide sequence