Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0156 and Shewana3_0157 overlap by 20 nucleotides Shewana3_0157 and Shewana3_0158 overlap by 4 nucleotides Shewana3_0158 and Shewana3_0159 are separated by 48 nucleotides
Shewana3_0156: Shewana3_0156 - general secretion pathway protein I (RefSeq), at 180,965 to 181,333
_0156
Shewana3_0157: Shewana3_0157 - general secretion pathway protein J (RefSeq), at 181,314 to 182,081
_0157
Shewana3_0158: Shewana3_0158 - general secretion pathway protein K (RefSeq), at 182,078 to 183,076
_0158
Shewana3_0159: Shewana3_0159 - general secretion pathway protein L (RefSeq), at 183,125 to 184,312
_0159
Position (kb) 182
183
184 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 181.199 kb on + strand, within Shewana3_0156 at 181.303 kb on + strand at 181.353 kb on - strand at 181.423 kb on - strand, within Shewana3_0157 at 181.423 kb on - strand, within Shewana3_0157 at 181.423 kb on - strand, within Shewana3_0157 at 181.425 kb on + strand, within Shewana3_0157 at 181.425 kb on + strand, within Shewana3_0157 at 181.541 kb on + strand, within Shewana3_0157 at 181.558 kb on + strand, within Shewana3_0157 at 181.568 kb on + strand, within Shewana3_0157 at 181.576 kb on - strand, within Shewana3_0157 at 181.671 kb on - strand, within Shewana3_0157 at 181.855 kb on - strand, within Shewana3_0157 at 181.856 kb on - strand, within Shewana3_0157 at 181.979 kb on + strand, within Shewana3_0157 at 182.082 kb on - strand at 182.202 kb on + strand, within Shewana3_0158 at 182.208 kb on + strand, within Shewana3_0158 at 182.290 kb on - strand, within Shewana3_0158 at 182.300 kb on - strand, within Shewana3_0158 at 182.484 kb on - strand, within Shewana3_0158 at 182.752 kb on + strand, within Shewana3_0158 at 182.753 kb on - strand, within Shewana3_0158 at 182.869 kb on + strand, within Shewana3_0158 at 182.919 kb on - strand, within Shewana3_0158 at 183.175 kb on - strand at 183.224 kb on - strand at 183.594 kb on + strand, within Shewana3_0159 at 183.602 kb on - strand, within Shewana3_0159 at 183.611 kb on + strand, within Shewana3_0159 at 183.634 kb on + strand, within Shewana3_0159 at 183.634 kb on + strand, within Shewana3_0159 at 183.634 kb on + strand, within Shewana3_0159 at 183.642 kb on - strand, within Shewana3_0159 at 183.674 kb on + strand, within Shewana3_0159 at 183.802 kb on - strand, within Shewana3_0159 at 183.805 kb on + strand, within Shewana3_0159 at 183.893 kb on + strand, within Shewana3_0159 at 183.893 kb on + strand, within Shewana3_0159 at 183.893 kb on + strand, within Shewana3_0159 at 183.893 kb on + strand, within Shewana3_0159 at 183.901 kb on - strand, within Shewana3_0159 at 183.921 kb on - strand, within Shewana3_0159 at 183.964 kb on + strand, within Shewana3_0159 at 184.020 kb on - strand, within Shewana3_0159 at 184.049 kb on + strand, within Shewana3_0159 at 184.057 kb on - strand, within Shewana3_0159 at 184.057 kb on - strand, within Shewana3_0159
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 181,199 + Shewana3_0156 0.63 +4.7 181,303 + -0.3 181,353 - +0.7 181,423 - Shewana3_0157 0.14 +3.7 181,423 - Shewana3_0157 0.14 +1.8 181,423 - Shewana3_0157 0.14 +3.7 181,425 + Shewana3_0157 0.14 +4.6 181,425 + Shewana3_0157 0.14 +3.5 181,541 + Shewana3_0157 0.30 +2.4 181,558 + Shewana3_0157 0.32 +3.1 181,568 + Shewana3_0157 0.33 +4.2 181,576 - Shewana3_0157 0.34 -0.1 181,671 - Shewana3_0157 0.46 +3.8 181,855 - Shewana3_0157 0.70 +4.1 181,856 - Shewana3_0157 0.71 +3.8 181,979 + Shewana3_0157 0.87 +1.1 182,082 - +4.0 182,202 + Shewana3_0158 0.12 +4.5 182,208 + Shewana3_0158 0.13 +3.2 182,290 - Shewana3_0158 0.21 +2.4 182,300 - Shewana3_0158 0.22 +1.4 182,484 - Shewana3_0158 0.41 +0.5 182,752 + Shewana3_0158 0.67 +2.9 182,753 - Shewana3_0158 0.68 +1.5 182,869 + Shewana3_0158 0.79 +1.9 182,919 - Shewana3_0158 0.84 +3.1 183,175 - +3.8 183,224 - +4.3 183,594 + Shewana3_0159 0.39 +3.1 183,602 - Shewana3_0159 0.40 +3.5 183,611 + Shewana3_0159 0.41 +4.7 183,634 + Shewana3_0159 0.43 +3.2 183,634 + Shewana3_0159 0.43 +3.8 183,634 + Shewana3_0159 0.43 +2.9 183,642 - Shewana3_0159 0.44 +3.7 183,674 + Shewana3_0159 0.46 +1.8 183,802 - Shewana3_0159 0.57 +3.8 183,805 + Shewana3_0159 0.57 +1.9 183,893 + Shewana3_0159 0.65 +4.0 183,893 + Shewana3_0159 0.65 +2.7 183,893 + Shewana3_0159 0.65 +3.7 183,893 + Shewana3_0159 0.65 +4.1 183,901 - Shewana3_0159 0.65 +3.0 183,921 - Shewana3_0159 0.67 +3.6 183,964 + Shewana3_0159 0.71 +1.8 184,020 - Shewana3_0159 0.75 +3.2 184,049 + Shewana3_0159 0.78 +2.7 184,057 - Shewana3_0159 0.78 +0.6 184,057 - Shewana3_0159 0.78 +4.6
Or see this region's nucleotide sequence