Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0152 and Shewana3_0153 are separated by 3 nucleotides Shewana3_0153 and Shewana3_0154 are separated by 69 nucleotides Shewana3_0154 and Shewana3_0155 are separated by 1 nucleotides
Shewana3_0152: Shewana3_0152 - type II secretion system protein E (GspE) (RefSeq), at 177,108 to 178,673
_0152
Shewana3_0153: Shewana3_0153 - general secretion pathway protein F (RefSeq), at 178,677 to 179,900
_0153
Shewana3_0154: Shewana3_0154 - general secretion pathway protein G (RefSeq), at 179,970 to 180,404
_0154
Shewana3_0155: Shewana3_0155 - general secretion pathway protein H (RefSeq), at 180,406 to 180,978
_0155
Position (kb) 178
179
180 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 177.934 kb on + strand, within Shewana3_0152 at 178.087 kb on - strand, within Shewana3_0152 at 178.108 kb on + strand, within Shewana3_0152 at 178.116 kb on - strand, within Shewana3_0152 at 178.138 kb on + strand, within Shewana3_0152 at 178.274 kb on + strand, within Shewana3_0152 at 178.358 kb on + strand, within Shewana3_0152 at 178.366 kb on - strand, within Shewana3_0152 at 178.390 kb on + strand, within Shewana3_0152 at 178.521 kb on - strand at 178.647 kb on + strand at 178.674 kb on + strand at 178.676 kb on - strand at 178.741 kb on - strand at 179.023 kb on + strand, within Shewana3_0153 at 179.031 kb on - strand, within Shewana3_0153 at 179.031 kb on - strand, within Shewana3_0153 at 179.474 kb on + strand, within Shewana3_0153 at 179.483 kb on + strand, within Shewana3_0153 at 179.491 kb on - strand, within Shewana3_0153 at 179.530 kb on + strand, within Shewana3_0153 at 179.598 kb on + strand, within Shewana3_0153 at 179.637 kb on + strand, within Shewana3_0153 at 179.654 kb on + strand, within Shewana3_0153 at 179.666 kb on + strand, within Shewana3_0153 at 179.698 kb on + strand, within Shewana3_0153 at 179.711 kb on - strand, within Shewana3_0153 at 179.728 kb on - strand, within Shewana3_0153 at 179.786 kb on + strand at 179.995 kb on + strand at 180.294 kb on + strand, within Shewana3_0154 at 180.314 kb on - strand, within Shewana3_0154 at 180.329 kb on + strand, within Shewana3_0154 at 180.405 kb on + strand at 180.413 kb on - strand at 180.683 kb on - strand, within Shewana3_0155 at 180.748 kb on + strand, within Shewana3_0155 at 180.792 kb on + strand, within Shewana3_0155 at 180.877 kb on - strand, within Shewana3_0155
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 177,934 + Shewana3_0152 0.53 +2.6 178,087 - Shewana3_0152 0.63 +1.4 178,108 + Shewana3_0152 0.64 +2.4 178,116 - Shewana3_0152 0.64 +1.5 178,138 + Shewana3_0152 0.66 +5.0 178,274 + Shewana3_0152 0.74 +3.9 178,358 + Shewana3_0152 0.80 +2.5 178,366 - Shewana3_0152 0.80 +3.6 178,390 + Shewana3_0152 0.82 +0.1 178,521 - +1.3 178,647 + +1.6 178,674 + -0.2 178,676 - -0.1 178,741 - +2.2 179,023 + Shewana3_0153 0.28 +5.6 179,031 - Shewana3_0153 0.29 +2.9 179,031 - Shewana3_0153 0.29 +3.2 179,474 + Shewana3_0153 0.65 +3.3 179,483 + Shewana3_0153 0.66 +1.1 179,491 - Shewana3_0153 0.67 +3.7 179,530 + Shewana3_0153 0.70 +1.8 179,598 + Shewana3_0153 0.75 +1.7 179,637 + Shewana3_0153 0.78 +1.4 179,654 + Shewana3_0153 0.80 +1.9 179,666 + Shewana3_0153 0.81 +4.6 179,698 + Shewana3_0153 0.83 +1.9 179,711 - Shewana3_0153 0.84 +1.9 179,728 - Shewana3_0153 0.86 +4.7 179,786 + +4.0 179,995 + +1.8 180,294 + Shewana3_0154 0.74 +1.9 180,314 - Shewana3_0154 0.79 +3.6 180,329 + Shewana3_0154 0.83 +1.3 180,405 + +0.4 180,413 - +4.4 180,683 - Shewana3_0155 0.48 +4.2 180,748 + Shewana3_0155 0.60 +2.0 180,792 + Shewana3_0155 0.67 +3.5 180,877 - Shewana3_0155 0.82 +2.5
Or see this region's nucleotide sequence