Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0120 and Shewana3_0121 are separated by 4 nucleotides Shewana3_0121 and Shewana3_0122 are separated by 63 nucleotides Shewana3_0122 and Shewana3_0123 are separated by 293 nucleotides
Shewana3_0120: Shewana3_0120 - hypothetical protein (RefSeq), at 135,406 to 135,855
_0120
Shewana3_0121: Shewana3_0121 - hypothetical protein (RefSeq), at 135,860 to 136,660
_0121
Shewana3_0122: Shewana3_0122 - hypothetical protein (RefSeq), at 136,724 to 138,367
_0122
Shewana3_0123: Shewana3_0123 - multiple antibiotic resistance (MarC)-related proteins (RefSeq), at 138,661 to 139,254
_0123
Position (kb)
136
137
138
139 Strain fitness (log2 ratio)
-2
-1
0
1 at 135.936 kb on - strand at 136.301 kb on - strand, within Shewana3_0121 at 136.457 kb on - strand, within Shewana3_0121 at 136.510 kb on - strand, within Shewana3_0121 at 136.663 kb on - strand at 136.721 kb on + strand at 136.729 kb on - strand at 136.923 kb on - strand, within Shewana3_0122 at 137.026 kb on - strand, within Shewana3_0122 at 137.085 kb on + strand, within Shewana3_0122 at 137.319 kb on - strand, within Shewana3_0122 at 137.421 kb on - strand, within Shewana3_0122 at 137.640 kb on + strand, within Shewana3_0122 at 137.695 kb on - strand, within Shewana3_0122 at 137.768 kb on + strand, within Shewana3_0122 at 137.776 kb on - strand, within Shewana3_0122 at 137.811 kb on - strand, within Shewana3_0122 at 137.917 kb on + strand, within Shewana3_0122 at 138.003 kb on - strand, within Shewana3_0122 at 138.109 kb on + strand, within Shewana3_0122 at 138.117 kb on - strand, within Shewana3_0122 at 138.238 kb on + strand at 138.238 kb on + strand at 138.246 kb on - strand at 138.246 kb on - strand at 138.337 kb on - strand at 138.501 kb on + strand at 138.518 kb on + strand at 138.552 kb on + strand at 138.556 kb on + strand at 139.049 kb on + strand, within Shewana3_0123 at 139.199 kb on - strand at 139.204 kb on + strand at 139.268 kb on - strand at 139.319 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 135,936 - +0.5 136,301 - Shewana3_0121 0.55 -0.9 136,457 - Shewana3_0121 0.75 +0.0 136,510 - Shewana3_0121 0.81 -0.5 136,663 - +0.4 136,721 + -1.2 136,729 - +0.3 136,923 - Shewana3_0122 0.12 +0.9 137,026 - Shewana3_0122 0.18 +0.6 137,085 + Shewana3_0122 0.22 +1.2 137,319 - Shewana3_0122 0.36 +0.6 137,421 - Shewana3_0122 0.42 +0.6 137,640 + Shewana3_0122 0.56 +0.9 137,695 - Shewana3_0122 0.59 +0.3 137,768 + Shewana3_0122 0.64 +0.9 137,776 - Shewana3_0122 0.64 +0.3 137,811 - Shewana3_0122 0.66 -0.7 137,917 + Shewana3_0122 0.73 +1.3 138,003 - Shewana3_0122 0.78 +1.0 138,109 + Shewana3_0122 0.84 +0.6 138,117 - Shewana3_0122 0.85 -0.9 138,238 + -1.4 138,238 + -1.4 138,246 - +0.1 138,246 - +1.0 138,337 - -1.6 138,501 + -1.7 138,518 + -0.6 138,552 + +0.4 138,556 + -0.3 139,049 + Shewana3_0123 0.65 -2.7 139,199 - -1.0 139,204 + +0.5 139,268 - +0.7 139,319 + -1.6
Or see this region's nucleotide sequence