Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0037 and Shewana3_0038 are separated by 2 nucleotides Shewana3_0038 and Shewana3_0039 are separated by 122 nucleotides Shewana3_0039 and Shewana3_0040 are separated by 129 nucleotides
Shewana3_0037: Shewana3_0037 - DNA protecting protein DprA (RefSeq), at 44,184 to 45,200
_0037
Shewana3_0038: Shewana3_0038 - hypothetical protein (RefSeq), at 45,203 to 45,679
_0038
Shewana3_0039: Shewana3_0039 - DNA topoisomerase type IA Zn finger domain-containing protein (RefSeq), at 45,802 to 46,365
_0039
Shewana3_0040: Shewana3_0040 - translation factor SUA5 (RefSeq), at 46,495 to 47,061
_0040
Position (kb) 45
46 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 44.258 kb on - strand at 44.261 kb on - strand at 44.319 kb on - strand, within Shewana3_0037 at 44.348 kb on + strand, within Shewana3_0037 at 44.356 kb on - strand, within Shewana3_0037 at 44.410 kb on + strand, within Shewana3_0037 at 44.452 kb on + strand, within Shewana3_0037 at 44.496 kb on + strand, within Shewana3_0037 at 44.504 kb on - strand, within Shewana3_0037 at 44.573 kb on - strand, within Shewana3_0037 at 44.583 kb on - strand, within Shewana3_0037 at 44.614 kb on - strand, within Shewana3_0037 at 44.777 kb on + strand, within Shewana3_0037 at 44.812 kb on + strand, within Shewana3_0037 at 44.919 kb on + strand, within Shewana3_0037 at 44.937 kb on - strand, within Shewana3_0037 at 45.006 kb on - strand, within Shewana3_0037 at 45.182 kb on - strand at 45.375 kb on + strand, within Shewana3_0038 at 45.490 kb on + strand, within Shewana3_0038 at 45.527 kb on + strand, within Shewana3_0038 at 45.652 kb on + strand at 45.660 kb on - strand at 45.660 kb on - strand at 45.830 kb on - strand at 45.867 kb on + strand, within Shewana3_0039 at 45.874 kb on + strand, within Shewana3_0039 at 45.982 kb on + strand, within Shewana3_0039 at 46.005 kb on + strand, within Shewana3_0039 at 46.038 kb on + strand, within Shewana3_0039 at 46.106 kb on + strand, within Shewana3_0039 at 46.122 kb on + strand, within Shewana3_0039 at 46.128 kb on + strand, within Shewana3_0039 at 46.130 kb on - strand, within Shewana3_0039 at 46.137 kb on + strand, within Shewana3_0039 at 46.171 kb on - strand, within Shewana3_0039 at 46.368 kb on + strand at 46.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 44,258 - +0.7 44,261 - -1.6 44,319 - Shewana3_0037 0.13 +0.6 44,348 + Shewana3_0037 0.16 -1.6 44,356 - Shewana3_0037 0.17 +1.0 44,410 + Shewana3_0037 0.22 +0.3 44,452 + Shewana3_0037 0.26 +0.4 44,496 + Shewana3_0037 0.31 +1.1 44,504 - Shewana3_0037 0.31 -1.7 44,573 - Shewana3_0037 0.38 +0.1 44,583 - Shewana3_0037 0.39 -0.8 44,614 - Shewana3_0037 0.42 -0.1 44,777 + Shewana3_0037 0.58 +0.5 44,812 + Shewana3_0037 0.62 +1.1 44,919 + Shewana3_0037 0.72 -1.5 44,937 - Shewana3_0037 0.74 +0.8 45,006 - Shewana3_0037 0.81 +1.3 45,182 - -0.0 45,375 + Shewana3_0038 0.36 +1.4 45,490 + Shewana3_0038 0.60 +1.1 45,527 + Shewana3_0038 0.68 +0.3 45,652 + -0.4 45,660 - +1.6 45,660 - +2.9 45,830 - -1.3 45,867 + Shewana3_0039 0.12 +0.8 45,874 + Shewana3_0039 0.13 -1.4 45,982 + Shewana3_0039 0.32 +0.4 46,005 + Shewana3_0039 0.36 -0.3 46,038 + Shewana3_0039 0.42 -2.6 46,106 + Shewana3_0039 0.54 -0.1 46,122 + Shewana3_0039 0.57 +0.3 46,128 + Shewana3_0039 0.58 +0.1 46,130 - Shewana3_0039 0.58 +0.5 46,137 + Shewana3_0039 0.59 +0.5 46,171 - Shewana3_0039 0.65 +0.8 46,368 + +0.4 46,409 - +0.9
Or see this region's nucleotide sequence