Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4355 and Shewana3_4356 are separated by 200 nucleotides Shewana3_4356 and Shewana3_4357 are separated by 9 nucleotides Shewana3_4357 and Shewana3_4358 are separated by 857 nucleotides
Shewana3_4355: Shewana3_4355 - hypothetical protein (RefSeq), at 240,677 to 241,708
_4355
Shewana3_4356: Shewana3_4356 - XRE family transcriptional regulator (RefSeq), at 241,909 to 242,253
_4356
Shewana3_4357: Shewana3_4357 - hypothetical protein (RefSeq), at 242,263 to 243,072
_4357
Shewana3_4358: Shewana3_4358 - phage integrase family protein (RefSeq), at 243,930 to 244,595
_4358
Position (kb) 242
243
244 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 241.379 kb on - strand, within Shewana3_4355 at 241.485 kb on + strand, within Shewana3_4355 at 241.493 kb on - strand, within Shewana3_4355 at 241.494 kb on + strand, within Shewana3_4355 at 241.617 kb on - strand at 241.621 kb on - strand at 241.629 kb on - strand at 241.643 kb on + strand at 241.644 kb on + strand at 241.652 kb on - strand at 241.774 kb on + strand at 241.774 kb on + strand at 241.782 kb on - strand at 241.786 kb on + strand at 241.986 kb on + strand, within Shewana3_4356 at 241.986 kb on + strand, within Shewana3_4356 at 242.044 kb on + strand, within Shewana3_4356 at 242.107 kb on - strand, within Shewana3_4356 at 242.423 kb on - strand, within Shewana3_4357 at 242.509 kb on + strand, within Shewana3_4357 at 242.518 kb on + strand, within Shewana3_4357 at 242.617 kb on + strand, within Shewana3_4357 at 242.638 kb on - strand, within Shewana3_4357 at 242.658 kb on + strand, within Shewana3_4357 at 242.786 kb on - strand, within Shewana3_4357 at 242.945 kb on + strand, within Shewana3_4357 at 243.462 kb on - strand at 243.495 kb on + strand at 243.695 kb on + strand at 243.890 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 241,379 - Shewana3_4355 0.68 -0.5 241,485 + Shewana3_4355 0.78 -0.9 241,493 - Shewana3_4355 0.79 -3.8 241,494 + Shewana3_4355 0.79 +1.1 241,617 - -1.7 241,621 - +1.1 241,629 - +0.9 241,643 + -2.2 241,644 + -1.9 241,652 - -1.8 241,774 + -1.4 241,774 + -1.6 241,782 - -0.3 241,786 + -1.5 241,986 + Shewana3_4356 0.22 -0.5 241,986 + Shewana3_4356 0.22 -0.3 242,044 + Shewana3_4356 0.39 -1.6 242,107 - Shewana3_4356 0.57 -3.5 242,423 - Shewana3_4357 0.20 +0.7 242,509 + Shewana3_4357 0.30 +0.1 242,518 + Shewana3_4357 0.31 +0.5 242,617 + Shewana3_4357 0.44 +0.3 242,638 - Shewana3_4357 0.46 +0.6 242,658 + Shewana3_4357 0.49 +0.8 242,786 - Shewana3_4357 0.65 -1.5 242,945 + Shewana3_4357 0.84 +0.8 243,462 - +0.6 243,495 + +0.6 243,695 + -1.7 243,890 + +0.5
Or see this region's nucleotide sequence