Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4339 and Shewana3_4340 are separated by 73 nucleotides Shewana3_4340 and Shewana3_4341 are separated by 407 nucleotides Shewana3_4341 and Shewana3_4342 are separated by 71 nucleotides
Shewana3_4339: Shewana3_4339 - integrase catalytic subunit (RefSeq), at 226,703 to 227,731
_4339
Shewana3_4340: Shewana3_4340 - hypothetical protein (RefSeq), at 227,805 to 228,281
_4340
Shewana3_4341: Shewana3_4341 - putative transcriptional regulator MerR (RefSeq), at 228,689 to 229,144
_4341
Shewana3_4342: Shewana3_4342 - putative mercuric transport protein (RefSeq), at 229,216 to 229,611
_4342
Position (kb) 227
228
229 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 226.818 kb on + strand, within Shewana3_4339 at 226.956 kb on + strand, within Shewana3_4339 at 226.956 kb on + strand, within Shewana3_4339 at 227.033 kb on - strand, within Shewana3_4339 at 227.093 kb on - strand, within Shewana3_4339 at 227.201 kb on - strand, within Shewana3_4339 at 227.565 kb on - strand, within Shewana3_4339 at 227.742 kb on - strand at 227.884 kb on - strand, within Shewana3_4340 at 227.945 kb on - strand, within Shewana3_4340 at 227.994 kb on + strand, within Shewana3_4340 at 228.215 kb on + strand, within Shewana3_4340 at 228.558 kb on + strand at 228.582 kb on + strand at 228.652 kb on + strand at 228.687 kb on + strand at 228.711 kb on - strand at 228.861 kb on + strand, within Shewana3_4341 at 228.946 kb on + strand, within Shewana3_4341 at 228.987 kb on + strand, within Shewana3_4341 at 229.077 kb on - strand, within Shewana3_4341 at 229.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 226,818 + Shewana3_4339 0.11 +0.0 226,956 + Shewana3_4339 0.25 -1.5 226,956 + Shewana3_4339 0.25 +0.4 227,033 - Shewana3_4339 0.32 +0.9 227,093 - Shewana3_4339 0.38 -1.5 227,201 - Shewana3_4339 0.48 -1.5 227,565 - Shewana3_4339 0.84 +1.2 227,742 - -4.2 227,884 - Shewana3_4340 0.17 +0.1 227,945 - Shewana3_4340 0.29 +0.7 227,994 + Shewana3_4340 0.40 +0.9 228,215 + Shewana3_4340 0.86 +0.4 228,558 + +0.6 228,582 + +0.4 228,652 + +0.5 228,687 + +0.5 228,711 - -2.2 228,861 + Shewana3_4341 0.38 -3.3 228,946 + Shewana3_4341 0.56 -2.4 228,987 + Shewana3_4341 0.65 +0.3 229,077 - Shewana3_4341 0.85 -2.9 229,240 - -0.8
Or see this region's nucleotide sequence