Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4336 and Shewana3_4337 are separated by 2 nucleotides Shewana3_4337 and Shewana3_4338 are separated by 186 nucleotides Shewana3_4338 and Shewana3_4339 are separated by 366 nucleotides
Shewana3_4336: Shewana3_4336 - AraC family transcriptional regulator (RefSeq), at 224,184 to 225,119
_4336
Shewana3_4337: Shewana3_4337 - hypothetical protein (RefSeq), at 225,122 to 225,862
_4337
Shewana3_4338: Shewana3_4338 - hypothetical protein (RefSeq), at 226,049 to 226,336
_4338
Shewana3_4339: Shewana3_4339 - integrase catalytic subunit (RefSeq), at 226,703 to 227,731
_4339
Position (kb) 226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 225.087 kb on - strand at 225.133 kb on + strand at 225.140 kb on + strand at 225.201 kb on - strand, within Shewana3_4337 at 225.300 kb on + strand, within Shewana3_4337 at 225.305 kb on + strand, within Shewana3_4337 at 225.402 kb on + strand, within Shewana3_4337 at 225.410 kb on - strand, within Shewana3_4337 at 225.410 kb on - strand, within Shewana3_4337 at 225.422 kb on + strand, within Shewana3_4337 at 225.592 kb on + strand, within Shewana3_4337 at 225.600 kb on - strand, within Shewana3_4337 at 225.699 kb on + strand, within Shewana3_4337 at 225.724 kb on - strand, within Shewana3_4337 at 225.724 kb on - strand, within Shewana3_4337 at 225.751 kb on - strand, within Shewana3_4337 at 225.856 kb on + strand at 225.997 kb on + strand at 226.004 kb on - strand at 226.116 kb on + strand, within Shewana3_4338 at 226.159 kb on - strand, within Shewana3_4338 at 226.437 kb on + strand at 226.680 kb on - strand at 226.818 kb on + strand, within Shewana3_4339 at 226.956 kb on + strand, within Shewana3_4339 at 226.956 kb on + strand, within Shewana3_4339 at 227.033 kb on - strand, within Shewana3_4339 at 227.093 kb on - strand, within Shewana3_4339 at 227.201 kb on - strand, within Shewana3_4339
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 225,087 - -0.3 225,133 + +1.1 225,140 + +0.4 225,201 - Shewana3_4337 0.11 +0.5 225,300 + Shewana3_4337 0.24 -0.4 225,305 + Shewana3_4337 0.25 +0.4 225,402 + Shewana3_4337 0.38 +0.7 225,410 - Shewana3_4337 0.39 -1.9 225,410 - Shewana3_4337 0.39 +0.8 225,422 + Shewana3_4337 0.40 -0.1 225,592 + Shewana3_4337 0.63 -0.1 225,600 - Shewana3_4337 0.65 +0.5 225,699 + Shewana3_4337 0.78 +0.2 225,724 - Shewana3_4337 0.81 +0.0 225,724 - Shewana3_4337 0.81 -2.9 225,751 - Shewana3_4337 0.85 -0.7 225,856 + +0.5 225,997 + -0.7 226,004 - -0.2 226,116 + Shewana3_4338 0.23 +1.3 226,159 - Shewana3_4338 0.38 +0.5 226,437 + -1.3 226,680 - +0.5 226,818 + Shewana3_4339 0.11 +0.0 226,956 + Shewana3_4339 0.25 -1.5 226,956 + Shewana3_4339 0.25 +0.4 227,033 - Shewana3_4339 0.32 +0.9 227,093 - Shewana3_4339 0.38 -1.5 227,201 - Shewana3_4339 0.48 -1.5
Or see this region's nucleotide sequence