Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4265 and Shewana3_4266 overlap by 1 nucleotides Shewana3_4266 and Shewana3_4267 are separated by 9 nucleotides Shewana3_4267 and Shewana3_4268 are separated by 727 nucleotides
Shewana3_4265: Shewana3_4265 - relaxase (RefSeq), at 141,356 to 144,181
_4265
Shewana3_4266: Shewana3_4266 - hypothetical protein (RefSeq), at 144,181 to 144,900
_4266
Shewana3_4267: Shewana3_4267 - hypothetical protein (RefSeq), at 144,910 to 145,329
_4267
Shewana3_4268: Shewana3_4268 - hypothetical protein (RefSeq), at 146,057 to 147,124
_4268
Position (kb) 144
145
146 Strain fitness (log2 ratio) -2
-1
0
1 at 143.930 kb on + strand at 143.948 kb on - strand at 144.380 kb on + strand, within Shewana3_4266 at 144.395 kb on - strand, within Shewana3_4266 at 144.540 kb on + strand, within Shewana3_4266 at 144.584 kb on + strand, within Shewana3_4266 at 144.591 kb on + strand, within Shewana3_4266 at 144.620 kb on + strand, within Shewana3_4266 at 144.650 kb on + strand, within Shewana3_4266 at 144.823 kb on + strand, within Shewana3_4266 at 144.828 kb on + strand, within Shewana3_4266 at 144.889 kb on + strand at 144.972 kb on - strand, within Shewana3_4267 at 145.099 kb on - strand, within Shewana3_4267 at 145.124 kb on + strand, within Shewana3_4267 at 145.201 kb on - strand, within Shewana3_4267 at 145.239 kb on - strand, within Shewana3_4267 at 145.273 kb on - strand, within Shewana3_4267 at 145.393 kb on - strand at 145.811 kb on + strand at 145.819 kb on - strand at 146.000 kb on + strand at 146.252 kb on - strand, within Shewana3_4268
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 143,930 + -1.2 143,948 - +0.4 144,380 + Shewana3_4266 0.28 -2.0 144,395 - Shewana3_4266 0.30 +0.6 144,540 + Shewana3_4266 0.50 +0.6 144,584 + Shewana3_4266 0.56 -1.6 144,591 + Shewana3_4266 0.57 -1.9 144,620 + Shewana3_4266 0.61 +0.5 144,650 + Shewana3_4266 0.65 +0.7 144,823 + Shewana3_4266 0.89 -2.4 144,828 + Shewana3_4266 0.90 -0.1 144,889 + +0.9 144,972 - Shewana3_4267 0.15 -1.7 145,099 - Shewana3_4267 0.45 +0.5 145,124 + Shewana3_4267 0.51 -1.6 145,201 - Shewana3_4267 0.69 -1.4 145,239 - Shewana3_4267 0.78 -1.6 145,273 - Shewana3_4267 0.86 -0.2 145,393 - +0.3 145,811 + +1.0 145,819 - +0.3 146,000 + +0.5 146,252 - Shewana3_4268 0.18 +0.7
Or see this region's nucleotide sequence