Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4259 and Shewana3_4260 are separated by 47 nucleotides Shewana3_4260 and Shewana3_4261 are separated by 9 nucleotides Shewana3_4261 and Shewana3_4262 are separated by 71 nucleotides Shewana3_4262 and Shewana3_4263 are separated by 225 nucleotides
Shewana3_4259: Shewana3_4259 - putative periplasmic protein (RefSeq), at 136,041 to 137,258
_4259
Shewana3_4260: Shewana3_4260 - hypothetical protein (RefSeq), at 137,306 to 137,962
_4260
Shewana3_4261: Shewana3_4261 - hypothetical protein (RefSeq), at 137,972 to 138,256
_4261
Shewana3_4262: Shewana3_4262 - hypothetical protein (RefSeq), at 138,328 to 138,615
_4262
Shewana3_4263: Shewana3_4263 - hypothetical protein (RefSeq), at 138,841 to 139,476
_4263
Position (kb) 137
138
139 Strain fitness (log2 ratio) -1
0
1 at 137.303 kb on + strand at 137.333 kb on + strand at 137.333 kb on + strand at 137.333 kb on + strand at 137.402 kb on - strand, within Shewana3_4260 at 137.402 kb on - strand, within Shewana3_4260 at 137.471 kb on + strand, within Shewana3_4260 at 137.471 kb on + strand, within Shewana3_4260 at 137.540 kb on - strand, within Shewana3_4260 at 137.656 kb on + strand, within Shewana3_4260 at 137.738 kb on + strand, within Shewana3_4260 at 137.746 kb on - strand, within Shewana3_4260 at 137.834 kb on - strand, within Shewana3_4260 at 137.936 kb on - strand at 138.011 kb on + strand, within Shewana3_4261 at 138.036 kb on + strand, within Shewana3_4261 at 138.181 kb on + strand, within Shewana3_4261 at 138.833 kb on + strand at 138.839 kb on - strand at 138.889 kb on + strand at 138.964 kb on - strand, within Shewana3_4263 at 138.987 kb on + strand, within Shewana3_4263 at 138.995 kb on - strand, within Shewana3_4263 at 138.995 kb on - strand, within Shewana3_4263 at 138.995 kb on - strand, within Shewana3_4263 at 139.004 kb on + strand, within Shewana3_4263 at 139.125 kb on + strand, within Shewana3_4263 at 139.178 kb on - strand, within Shewana3_4263 at 139.196 kb on - strand, within Shewana3_4263
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 137,303 + -1.2 137,333 + -1.5 137,333 + +0.5 137,333 + +0.8 137,402 - Shewana3_4260 0.15 +0.3 137,402 - Shewana3_4260 0.15 -1.4 137,471 + Shewana3_4260 0.25 -1.5 137,471 + Shewana3_4260 0.25 -0.9 137,540 - Shewana3_4260 0.36 +1.0 137,656 + Shewana3_4260 0.53 +0.4 137,738 + Shewana3_4260 0.66 +0.9 137,746 - Shewana3_4260 0.67 -1.0 137,834 - Shewana3_4260 0.80 -0.8 137,936 - +0.5 138,011 + Shewana3_4261 0.14 +0.7 138,036 + Shewana3_4261 0.22 +1.0 138,181 + Shewana3_4261 0.73 -0.1 138,833 + +0.7 138,839 - +0.7 138,889 + +0.1 138,964 - Shewana3_4263 0.19 +0.2 138,987 + Shewana3_4263 0.23 -1.1 138,995 - Shewana3_4263 0.24 +0.4 138,995 - Shewana3_4263 0.24 -1.0 138,995 - Shewana3_4263 0.24 -1.1 139,004 + Shewana3_4263 0.26 -0.8 139,125 + Shewana3_4263 0.45 -1.0 139,178 - Shewana3_4263 0.53 +0.5 139,196 - Shewana3_4263 0.56 +0.2
Or see this region's nucleotide sequence