Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4207 and Shewana3_4208 are separated by 68 nucleotides Shewana3_4208 and Shewana3_4209 overlap by 20 nucleotides Shewana3_4209 and Shewana3_4210 are separated by 74 nucleotides
Shewana3_4207: Shewana3_4207 - TraU family protein (RefSeq), at 75,580 to 76,659
_4207
Shewana3_4208: Shewana3_4208 - hypothetical protein (RefSeq), at 76,728 to 78,023
_4208
Shewana3_4209: Shewana3_4209 - Type IV secretory pathway protease TraF-like protein (RefSeq), at 78,004 to 78,510
_4209
Shewana3_4210: Shewana3_4210 - hypothetical protein (RefSeq), at 78,585 to 79,010
_4210
Position (kb) 76
77
78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 75.841 kb on - strand, within Shewana3_4207 at 75.901 kb on + strand, within Shewana3_4207 at 75.908 kb on + strand, within Shewana3_4207 at 75.916 kb on - strand, within Shewana3_4207 at 76.424 kb on - strand, within Shewana3_4207 at 76.429 kb on - strand, within Shewana3_4207 at 76.598 kb on - strand at 76.788 kb on + strand at 76.792 kb on - strand at 76.827 kb on - strand at 76.957 kb on + strand, within Shewana3_4208 at 76.965 kb on - strand, within Shewana3_4208 at 77.078 kb on - strand, within Shewana3_4208 at 77.144 kb on - strand, within Shewana3_4208 at 77.185 kb on + strand, within Shewana3_4208 at 77.315 kb on + strand, within Shewana3_4208 at 77.342 kb on - strand, within Shewana3_4208 at 77.372 kb on + strand, within Shewana3_4208 at 77.401 kb on - strand, within Shewana3_4208 at 77.402 kb on - strand, within Shewana3_4208 at 77.417 kb on + strand, within Shewana3_4208 at 77.742 kb on - strand, within Shewana3_4208 at 77.851 kb on - strand, within Shewana3_4208 at 77.854 kb on - strand, within Shewana3_4208 at 77.856 kb on - strand, within Shewana3_4208 at 78.081 kb on + strand, within Shewana3_4209 at 78.081 kb on + strand, within Shewana3_4209 at 78.089 kb on - strand, within Shewana3_4209 at 78.120 kb on - strand, within Shewana3_4209 at 78.210 kb on + strand, within Shewana3_4209 at 78.210 kb on + strand, within Shewana3_4209 at 78.218 kb on - strand, within Shewana3_4209 at 78.316 kb on - strand, within Shewana3_4209 at 78.491 kb on + strand at 78.774 kb on + strand, within Shewana3_4210 at 78.900 kb on - strand, within Shewana3_4210 at 78.900 kb on - strand, within Shewana3_4210 at 79.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 75,841 - Shewana3_4207 0.24 +0.6 75,901 + Shewana3_4207 0.30 +0.6 75,908 + Shewana3_4207 0.30 -1.8 75,916 - Shewana3_4207 0.31 +0.6 76,424 - Shewana3_4207 0.78 +0.7 76,429 - Shewana3_4207 0.79 +0.7 76,598 - -1.6 76,788 + +0.3 76,792 - +0.7 76,827 - -1.5 76,957 + Shewana3_4208 0.18 +1.1 76,965 - Shewana3_4208 0.18 -2.8 77,078 - Shewana3_4208 0.27 -0.3 77,144 - Shewana3_4208 0.32 +0.2 77,185 + Shewana3_4208 0.35 -1.8 77,315 + Shewana3_4208 0.45 -2.4 77,342 - Shewana3_4208 0.47 +0.6 77,372 + Shewana3_4208 0.50 +0.2 77,401 - Shewana3_4208 0.52 +0.4 77,402 - Shewana3_4208 0.52 -1.0 77,417 + Shewana3_4208 0.53 +0.2 77,742 - Shewana3_4208 0.78 -3.2 77,851 - Shewana3_4208 0.87 -1.8 77,854 - Shewana3_4208 0.87 +0.5 77,856 - Shewana3_4208 0.87 -1.0 78,081 + Shewana3_4209 0.15 -0.4 78,081 + Shewana3_4209 0.15 -0.2 78,089 - Shewana3_4209 0.17 +0.4 78,120 - Shewana3_4209 0.23 +0.2 78,210 + Shewana3_4209 0.41 +1.7 78,210 + Shewana3_4209 0.41 +0.6 78,218 - Shewana3_4209 0.42 +0.8 78,316 - Shewana3_4209 0.62 +0.6 78,491 + -1.7 78,774 + Shewana3_4210 0.44 +0.0 78,900 - Shewana3_4210 0.74 +0.4 78,900 - Shewana3_4210 0.74 -2.2 79,020 - +1.3
Or see this region's nucleotide sequence