Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4201 and Shewana3_4202 are separated by 96 nucleotides Shewana3_4202 and Shewana3_4203 are separated by 178 nucleotides
Shewana3_4201: Shewana3_4201 - ATPase (RefSeq), at 63,987 to 64,961
_4201
Shewana3_4202: Shewana3_4202 - hypothetical protein (RefSeq), at 65,058 to 66,125
_4202
Shewana3_4203: Shewana3_4203 - hypothetical protein (RefSeq), at 66,304 to 66,699
_4203
Position (kb) 65
66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 64.073 kb on - strand at 64.281 kb on - strand, within Shewana3_4201 at 64.543 kb on - strand, within Shewana3_4201 at 64.591 kb on + strand, within Shewana3_4201 at 64.599 kb on - strand, within Shewana3_4201 at 64.690 kb on - strand, within Shewana3_4201 at 64.700 kb on - strand, within Shewana3_4201 at 64.839 kb on + strand, within Shewana3_4201 at 65.173 kb on - strand, within Shewana3_4202 at 65.187 kb on + strand, within Shewana3_4202 at 65.401 kb on - strand, within Shewana3_4202 at 65.424 kb on - strand, within Shewana3_4202 at 65.573 kb on + strand, within Shewana3_4202 at 65.642 kb on - strand, within Shewana3_4202 at 65.643 kb on + strand, within Shewana3_4202 at 65.705 kb on + strand, within Shewana3_4202 at 65.713 kb on - strand, within Shewana3_4202 at 65.825 kb on - strand, within Shewana3_4202 at 65.968 kb on - strand, within Shewana3_4202 at 66.140 kb on - strand at 66.234 kb on + strand at 66.332 kb on + strand at 66.508 kb on - strand, within Shewana3_4203 at 66.517 kb on - strand, within Shewana3_4203 at 66.703 kb on - strand at 66.731 kb on + strand at 66.731 kb on + strand at 66.739 kb on - strand at 66.784 kb on - strand at 66.815 kb on - strand at 66.903 kb on + strand at 67.102 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 64,073 - -1.6 64,281 - Shewana3_4201 0.30 -1.9 64,543 - Shewana3_4201 0.57 +0.9 64,591 + Shewana3_4201 0.62 +0.1 64,599 - Shewana3_4201 0.63 +0.8 64,690 - Shewana3_4201 0.72 +0.8 64,700 - Shewana3_4201 0.73 -0.1 64,839 + Shewana3_4201 0.87 -0.9 65,173 - Shewana3_4202 0.11 +1.2 65,187 + Shewana3_4202 0.12 +1.6 65,401 - Shewana3_4202 0.32 +0.5 65,424 - Shewana3_4202 0.34 +0.6 65,573 + Shewana3_4202 0.48 -1.3 65,642 - Shewana3_4202 0.55 -3.5 65,643 + Shewana3_4202 0.55 +0.9 65,705 + Shewana3_4202 0.61 +0.5 65,713 - Shewana3_4202 0.61 -1.4 65,825 - Shewana3_4202 0.72 +1.0 65,968 - Shewana3_4202 0.85 -0.3 66,140 - -0.7 66,234 + +0.3 66,332 + -0.0 66,508 - Shewana3_4203 0.52 +0.1 66,517 - Shewana3_4203 0.54 -1.1 66,703 - +1.0 66,731 + +0.8 66,731 + +0.5 66,739 - +1.1 66,784 - +0.3 66,815 - +0.3 66,903 + -0.1 67,102 + +0.3
Or see this region's nucleotide sequence