Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4198 and Shewana3_4199 are separated by 775 nucleotides Shewana3_4199 and Shewana3_4200 are separated by 189 nucleotides
Shewana3_4198: Shewana3_4198 - hypothetical protein (RefSeq), at 59,875 to 60,306
_4198
Shewana3_4199: Shewana3_4199 - hypothetical protein (RefSeq), at 61,082 to 62,101
_4199
Shewana3_4200: Shewana3_4200 - hypothetical protein (RefSeq), at 62,291 to 63,763
_4200
Position (kb) 61
62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 60.140 kb on - strand, within Shewana3_4198 at 60.330 kb on + strand at 60.353 kb on + strand at 60.355 kb on + strand at 60.362 kb on + strand at 60.363 kb on - strand at 60.370 kb on - strand at 60.370 kb on - strand at 60.372 kb on + strand at 60.372 kb on + strand at 60.372 kb on + strand at 60.467 kb on - strand at 60.501 kb on - strand at 60.527 kb on + strand at 60.548 kb on - strand at 61.001 kb on + strand at 61.009 kb on - strand at 61.009 kb on - strand at 61.188 kb on - strand, within Shewana3_4199 at 61.226 kb on + strand, within Shewana3_4199 at 61.234 kb on - strand, within Shewana3_4199 at 61.234 kb on - strand, within Shewana3_4199 at 61.234 kb on - strand, within Shewana3_4199 at 61.299 kb on + strand, within Shewana3_4199 at 61.618 kb on + strand, within Shewana3_4199 at 61.618 kb on + strand, within Shewana3_4199 at 61.618 kb on + strand, within Shewana3_4199 at 61.626 kb on - strand, within Shewana3_4199 at 61.626 kb on - strand, within Shewana3_4199 at 61.626 kb on - strand, within Shewana3_4199 at 61.643 kb on - strand, within Shewana3_4199 at 61.662 kb on - strand, within Shewana3_4199 at 61.662 kb on - strand, within Shewana3_4199 at 62.017 kb on - strand at 62.104 kb on - strand at 62.149 kb on - strand at 62.288 kb on - strand at 62.352 kb on - strand at 62.472 kb on - strand, within Shewana3_4200 at 62.589 kb on - strand, within Shewana3_4200 at 62.589 kb on - strand, within Shewana3_4200 at 62.599 kb on - strand, within Shewana3_4200 at 62.990 kb on + strand, within Shewana3_4200 at 63.011 kb on + strand, within Shewana3_4200 at 63.011 kb on + strand, within Shewana3_4200 at 63.029 kb on - strand, within Shewana3_4200 at 63.029 kb on - strand, within Shewana3_4200 at 63.090 kb on + strand, within Shewana3_4200
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 60,140 - Shewana3_4198 0.61 -1.7 60,330 + -2.0 60,353 + +1.5 60,355 + +0.3 60,362 + +0.4 60,363 - -1.5 60,370 - +0.4 60,370 - -0.1 60,372 + -1.3 60,372 + +0.5 60,372 + -3.3 60,467 - +1.5 60,501 - -0.0 60,527 + +0.1 60,548 - +0.1 61,001 + +0.5 61,009 - +0.5 61,009 - +0.8 61,188 - Shewana3_4199 0.10 +0.4 61,226 + Shewana3_4199 0.14 +0.3 61,234 - Shewana3_4199 0.15 +0.6 61,234 - Shewana3_4199 0.15 +1.1 61,234 - Shewana3_4199 0.15 -0.5 61,299 + Shewana3_4199 0.21 +0.8 61,618 + Shewana3_4199 0.53 +1.4 61,618 + Shewana3_4199 0.53 -0.5 61,618 + Shewana3_4199 0.53 +0.3 61,626 - Shewana3_4199 0.53 +0.3 61,626 - Shewana3_4199 0.53 +0.0 61,626 - Shewana3_4199 0.53 +0.1 61,643 - Shewana3_4199 0.55 +0.3 61,662 - Shewana3_4199 0.57 +0.5 61,662 - Shewana3_4199 0.57 +0.7 62,017 - +0.4 62,104 - +0.3 62,149 - +0.2 62,288 - +0.4 62,352 - +0.4 62,472 - Shewana3_4200 0.12 +0.5 62,589 - Shewana3_4200 0.20 +0.4 62,589 - Shewana3_4200 0.20 -0.7 62,599 - Shewana3_4200 0.21 +0.1 62,990 + Shewana3_4200 0.47 +0.2 63,011 + Shewana3_4200 0.49 +0.0 63,011 + Shewana3_4200 0.49 -0.4 63,029 - Shewana3_4200 0.50 -0.1 63,029 - Shewana3_4200 0.50 +1.5 63,090 + Shewana3_4200 0.54 -0.7
Or see this region's nucleotide sequence