Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4179 and Shewana3_4180 are separated by 10 nucleotides Shewana3_4180 and Shewana3_4181 are separated by 13 nucleotides Shewana3_4181 and Shewana3_4182 are separated by 354 nucleotides
Shewana3_4179: Shewana3_4179 - hypothetical protein (RefSeq), at 45,611 to 46,507
_4179
Shewana3_4180: Shewana3_4180 - hypothetical protein (RefSeq), at 46,518 to 47,165
_4180
Shewana3_4181: Shewana3_4181 - hypothetical protein (RefSeq), at 47,179 to 47,901
_4181
Shewana3_4182: Shewana3_4182 - hypothetical protein (RefSeq), at 48,256 to 48,957
_4182
Position (kb) 47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 46.289 kb on - strand, within Shewana3_4179 at 46.338 kb on - strand, within Shewana3_4179 at 46.419 kb on - strand at 46.431 kb on - strand at 46.608 kb on - strand, within Shewana3_4180 at 46.619 kb on + strand, within Shewana3_4180 at 46.762 kb on + strand, within Shewana3_4180 at 46.903 kb on + strand, within Shewana3_4180 at 46.947 kb on + strand, within Shewana3_4180 at 47.171 kb on - strand at 47.298 kb on + strand, within Shewana3_4181 at 47.414 kb on - strand, within Shewana3_4181 at 47.517 kb on + strand, within Shewana3_4181 at 47.547 kb on + strand, within Shewana3_4181 at 47.671 kb on + strand, within Shewana3_4181 at 47.689 kb on - strand, within Shewana3_4181 at 47.699 kb on - strand, within Shewana3_4181 at 48.385 kb on + strand, within Shewana3_4182 at 48.474 kb on - strand, within Shewana3_4182 at 48.609 kb on - strand, within Shewana3_4182 at 48.644 kb on + strand, within Shewana3_4182 at 48.652 kb on - strand, within Shewana3_4182
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 46,289 - Shewana3_4179 0.76 +0.2 46,338 - Shewana3_4179 0.81 +0.5 46,419 - +0.1 46,431 - +1.1 46,608 - Shewana3_4180 0.14 +0.2 46,619 + Shewana3_4180 0.16 +0.4 46,762 + Shewana3_4180 0.38 +0.1 46,903 + Shewana3_4180 0.59 +0.3 46,947 + Shewana3_4180 0.66 +0.4 47,171 - +0.6 47,298 + Shewana3_4181 0.16 -3.5 47,414 - Shewana3_4181 0.33 +1.4 47,517 + Shewana3_4181 0.47 +0.7 47,547 + Shewana3_4181 0.51 +0.9 47,671 + Shewana3_4181 0.68 +0.9 47,689 - Shewana3_4181 0.71 -0.9 47,699 - Shewana3_4181 0.72 +0.3 48,385 + Shewana3_4182 0.18 -0.1 48,474 - Shewana3_4182 0.31 -0.4 48,609 - Shewana3_4182 0.50 -0.0 48,644 + Shewana3_4182 0.55 -2.1 48,652 - Shewana3_4182 0.56 -1.2
Or see this region's nucleotide sequence