Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4173 and Shewana3_4174 are separated by 205 nucleotides Shewana3_4174 and Shewana3_4175 are separated by 240 nucleotides Shewana3_4175 and Shewana3_4176 are separated by 414 nucleotides
Shewana3_4173: Shewana3_4173 - putative prophage repressor (RefSeq), at 39,612 to 40,346
_4173
Shewana3_4174: Shewana3_4174 - hypothetical protein (RefSeq), at 40,552 to 40,911
_4174
Shewana3_4175: Shewana3_4175 - hypothetical protein (RefSeq), at 41,152 to 42,258
_4175
Shewana3_4176: Shewana3_4176 - hypothetical protein (RefSeq), at 42,673 to 43,521
_4176
Position (kb) 41
42
43 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 40.203 kb on - strand, within Shewana3_4173 at 40.244 kb on + strand, within Shewana3_4173 at 40.304 kb on - strand at 40.459 kb on + strand at 40.467 kb on - strand at 40.477 kb on - strand at 40.526 kb on + strand at 40.544 kb on - strand at 40.544 kb on - strand at 40.544 kb on - strand at 40.544 kb on - strand at 40.573 kb on - strand at 40.791 kb on - strand, within Shewana3_4174 at 40.840 kb on - strand, within Shewana3_4174 at 40.874 kb on - strand, within Shewana3_4174 at 40.881 kb on + strand at 41.088 kb on - strand at 41.271 kb on - strand, within Shewana3_4175 at 41.863 kb on + strand, within Shewana3_4175 at 41.917 kb on + strand, within Shewana3_4175 at 42.066 kb on + strand, within Shewana3_4175 at 42.122 kb on + strand, within Shewana3_4175 at 42.125 kb on - strand, within Shewana3_4175 at 42.383 kb on + strand at 42.391 kb on - strand at 42.391 kb on - strand at 42.435 kb on + strand at 42.443 kb on - strand at 42.443 kb on - strand at 42.443 kb on - strand at 42.629 kb on + strand at 42.630 kb on + strand at 42.630 kb on + strand at 42.650 kb on - strand at 42.765 kb on - strand, within Shewana3_4176 at 43.253 kb on + strand, within Shewana3_4176 at 43.253 kb on + strand, within Shewana3_4176
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 40,203 - Shewana3_4173 0.80 +0.7 40,244 + Shewana3_4173 0.86 +0.3 40,304 - -1.5 40,459 + -1.6 40,467 - +0.2 40,477 - +1.1 40,526 + -2.3 40,544 - +0.4 40,544 - -0.1 40,544 - +0.1 40,544 - -1.4 40,573 - +0.4 40,791 - Shewana3_4174 0.66 -4.4 40,840 - Shewana3_4174 0.80 +0.2 40,874 - Shewana3_4174 0.89 -0.3 40,881 + -0.2 41,088 - -1.6 41,271 - Shewana3_4175 0.11 +0.6 41,863 + Shewana3_4175 0.64 +0.1 41,917 + Shewana3_4175 0.69 -2.8 42,066 + Shewana3_4175 0.83 -2.1 42,122 + Shewana3_4175 0.88 +1.3 42,125 - Shewana3_4175 0.88 +0.0 42,383 + -1.7 42,391 - -0.6 42,391 - -2.0 42,435 + +0.6 42,443 - +0.6 42,443 - -2.8 42,443 - +1.1 42,629 + +0.4 42,630 + +0.9 42,630 + +0.4 42,650 - -0.6 42,765 - Shewana3_4176 0.11 -1.4 43,253 + Shewana3_4176 0.68 +1.2 43,253 + Shewana3_4176 0.68 +0.4
Or see this region's nucleotide sequence