Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dut and slmA are separated by 130 nucleotides slmA and Shew_3485 are separated by 149 nucleotides Shew_3485 and folE are separated by 225 nucleotides folE and pyrE are separated by 81 nucleotides
Shew_3483: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (RefSeq), at 4,168,723 to 4,169,181
dut
Shew_3484: slmA - nucleoid occlusion protein (RefSeq), at 4,169,312 to 4,169,905
slmA
Shew_3485: Shew_3485 - peptidase S9 prolyl oligopeptidase (RefSeq), at 4,170,055 to 4,172,019
_3485
Shew_3486: folE - GTP cyclohydrolase I (RefSeq), at 4,172,245 to 4,172,889
folE
Shew_3487: pyrE - orotate phosphoribosyltransferase (RefSeq), at 4,172,971 to 4,173,612
pyrE
Position (kb) 4170
4171
4172
4173 Strain fitness (log2 ratio) -2
-1
0
1 at 4169.174 kb on - strand at 4169.256 kb on + strand at 4169.759 kb on - strand, within slmA at 4169.867 kb on - strand at 4170.290 kb on + strand, within Shew_3485 at 4170.378 kb on + strand, within Shew_3485 at 4170.396 kb on - strand, within Shew_3485 at 4170.421 kb on - strand, within Shew_3485 at 4170.603 kb on + strand, within Shew_3485 at 4170.611 kb on - strand, within Shew_3485 at 4170.754 kb on + strand, within Shew_3485 at 4170.773 kb on - strand, within Shew_3485 at 4170.826 kb on + strand, within Shew_3485 at 4170.834 kb on - strand, within Shew_3485 at 4170.867 kb on + strand, within Shew_3485 at 4171.229 kb on + strand, within Shew_3485 at 4171.367 kb on + strand, within Shew_3485 at 4171.367 kb on + strand, within Shew_3485 at 4171.375 kb on - strand, within Shew_3485 at 4172.116 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 4,169,174 - -2.6 4,169,256 + -0.2 4,169,759 - slmA Shew_3484 0.75 -1.6 4,169,867 - -2.5 4,170,290 + Shew_3485 0.12 -1.0 4,170,378 + Shew_3485 0.16 -0.5 4,170,396 - Shew_3485 0.17 +0.8 4,170,421 - Shew_3485 0.19 +0.4 4,170,603 + Shew_3485 0.28 +1.0 4,170,611 - Shew_3485 0.28 +1.0 4,170,754 + Shew_3485 0.36 +0.6 4,170,773 - Shew_3485 0.37 +0.0 4,170,826 + Shew_3485 0.39 +0.1 4,170,834 - Shew_3485 0.40 +1.5 4,170,867 + Shew_3485 0.41 +0.4 4,171,229 + Shew_3485 0.60 +1.4 4,171,367 + Shew_3485 0.67 +0.0 4,171,367 + Shew_3485 0.67 -0.8 4,171,375 - Shew_3485 0.67 -0.8 4,172,116 + +0.7
Or see this region's nucleotide sequence