Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3062 and Shew_3063 are separated by 168 nucleotides Shew_3063 and gcvT are separated by 193 nucleotides gcvT and Shew_3065 are separated by 476 nucleotides
Shew_3062: Shew_3062 - glycine dehydrogenase (RefSeq), at 3,635,485 to 3,638,373
_3062
Shew_3063: Shew_3063 - glycine cleavage system protein H (RefSeq), at 3,638,542 to 3,638,931
_3063
Shew_3064: gcvT - glycine cleavage system aminomethyltransferase T (RefSeq), at 3,639,125 to 3,640,219
gcvT
Shew_3065: Shew_3065 - UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase (RefSeq), at 3,640,696 to 3,641,904
_3065
Position (kb)
3639
3640
3641 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3638.199 kb on + strand at 3638.864 kb on - strand, within Shew_3063 at 3639.113 kb on - strand at 3639.136 kb on + strand at 3639.267 kb on - strand, within gcvT at 3639.306 kb on - strand, within gcvT at 3639.359 kb on - strand, within gcvT at 3639.437 kb on + strand, within gcvT at 3639.518 kb on + strand, within gcvT at 3639.526 kb on - strand, within gcvT at 3639.576 kb on - strand, within gcvT at 3639.683 kb on + strand, within gcvT at 3639.859 kb on - strand, within gcvT at 3640.398 kb on - strand at 3640.522 kb on - strand at 3640.522 kb on - strand at 3640.730 kb on + strand at 3640.730 kb on + strand at 3641.024 kb on - strand, within Shew_3065 at 3641.099 kb on + strand, within Shew_3065 at 3641.134 kb on - strand, within Shew_3065
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 3,638,199 + +0.5 3,638,864 - Shew_3063 0.83 +1.7 3,639,113 - +2.4 3,639,136 + +1.9 3,639,267 - gcvT Shew_3064 0.13 +1.9 3,639,306 - gcvT Shew_3064 0.17 +1.3 3,639,359 - gcvT Shew_3064 0.21 +1.5 3,639,437 + gcvT Shew_3064 0.28 +1.6 3,639,518 + gcvT Shew_3064 0.36 +1.4 3,639,526 - gcvT Shew_3064 0.37 +1.7 3,639,576 - gcvT Shew_3064 0.41 +2.0 3,639,683 + gcvT Shew_3064 0.51 +2.3 3,639,859 - gcvT Shew_3064 0.67 +2.0 3,640,398 - +1.5 3,640,522 - +0.5 3,640,522 - +1.0 3,640,730 + -3.1 3,640,730 + +0.3 3,641,024 - Shew_3065 0.27 +1.7 3,641,099 + Shew_3065 0.33 +1.0 3,641,134 - Shew_3065 0.36 +1.5
Or see this region's nucleotide sequence