Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1540 and Shew_1541 are separated by 60 nucleotides Shew_1541 and Shew_1542 are separated by 22 nucleotides Shew_1542 and Shew_1543 are separated by 14 nucleotides
Shew_1540: Shew_1540 - alcohol dehydrogenase (RefSeq), at 1,768,212 to 1,769,342
_1540
Shew_1541: Shew_1541 - carboxylesterase (RefSeq), at 1,769,403 to 1,770,239
_1541
Shew_1542: Shew_1542 - major facilitator transporter (RefSeq), at 1,770,262 to 1,771,800
_1542
Shew_1543: Shew_1543 - secretion protein HlyD family protein (RefSeq), at 1,771,815 to 1,772,873
_1543
Position (kb)
1770
1771
1772 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1769.859 kb on + strand, within Shew_1541 at 1770.059 kb on + strand, within Shew_1541 at 1770.167 kb on - strand at 1770.183 kb on + strand at 1770.313 kb on + strand at 1770.403 kb on + strand at 1770.403 kb on + strand at 1770.905 kb on + strand, within Shew_1542 at 1771.010 kb on + strand, within Shew_1542 at 1771.072 kb on + strand, within Shew_1542 at 1771.080 kb on - strand, within Shew_1542 at 1771.563 kb on - strand, within Shew_1542 at 1771.839 kb on - strand at 1772.000 kb on - strand, within Shew_1543 at 1772.251 kb on + strand, within Shew_1543 at 1772.251 kb on + strand, within Shew_1543 at 1772.259 kb on - strand, within Shew_1543 at 1772.259 kb on - strand, within Shew_1543 at 1772.439 kb on + strand, within Shew_1543 at 1772.573 kb on - strand, within Shew_1543 at 1772.588 kb on - strand, within Shew_1543 at 1772.628 kb on + strand, within Shew_1543 at 1772.735 kb on - strand, within Shew_1543
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 1,769,859 + Shew_1541 0.54 +0.2 1,770,059 + Shew_1541 0.78 +0.4 1,770,167 - -0.3 1,770,183 + +0.4 1,770,313 + +0.2 1,770,403 + -2.6 1,770,403 + -0.3 1,770,905 + Shew_1542 0.42 +0.4 1,771,010 + Shew_1542 0.49 +1.8 1,771,072 + Shew_1542 0.53 +0.2 1,771,080 - Shew_1542 0.53 +1.7 1,771,563 - Shew_1542 0.85 +0.7 1,771,839 - -3.4 1,772,000 - Shew_1543 0.17 -0.4 1,772,251 + Shew_1543 0.41 +0.1 1,772,251 + Shew_1543 0.41 +0.5 1,772,259 - Shew_1543 0.42 +0.5 1,772,259 - Shew_1543 0.42 -0.8 1,772,439 + Shew_1543 0.59 -0.3 1,772,573 - Shew_1543 0.72 +2.8 1,772,588 - Shew_1543 0.73 +0.5 1,772,628 + Shew_1543 0.77 -0.3 1,772,735 - Shew_1543 0.87 -0.0
Or see this region's nucleotide sequence