Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1451 and Shew_1452 are separated by 242 nucleotides Shew_1452 and Shew_1453 are separated by 65 nucleotides Shew_1453 and Shew_1454 are separated by 114 nucleotides
Shew_1451: Shew_1451 - ABC transporter-related protein (RefSeq), at 1,671,372 to 1,673,201
_1451
Shew_1452: Shew_1452 - AraC family transcriptional regulator (RefSeq), at 1,673,444 to 1,674,955
_1452
Shew_1453: Shew_1453 - methylated-DNA--protein-cysteinemethyltransferase (RefSeq), at 1,675,021 to 1,675,608
_1453
Shew_1454: Shew_1454 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 1,675,723 to 1,677,048
_1454
Position (kb)
1673
1674
1675 Strain fitness (log2 ratio)
-1
0
1
2 at 1672.485 kb on + strand, within Shew_1451 at 1672.644 kb on - strand, within Shew_1451 at 1672.746 kb on - strand, within Shew_1451 at 1672.758 kb on + strand, within Shew_1451 at 1673.050 kb on - strand at 1673.472 kb on - strand at 1673.663 kb on + strand, within Shew_1452 at 1673.778 kb on - strand, within Shew_1452 at 1673.922 kb on + strand, within Shew_1452 at 1674.090 kb on + strand, within Shew_1452 at 1674.339 kb on + strand, within Shew_1452 at 1674.366 kb on - strand, within Shew_1452 at 1674.366 kb on - strand, within Shew_1452 at 1674.795 kb on - strand, within Shew_1452 at 1674.945 kb on - strand at 1675.256 kb on - strand, within Shew_1453 at 1675.321 kb on - strand, within Shew_1453 at 1675.414 kb on - strand, within Shew_1453 at 1675.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 1,672,485 + Shew_1451 0.61 -0.8 1,672,644 - Shew_1451 0.70 +1.0 1,672,746 - Shew_1451 0.75 -0.9 1,672,758 + Shew_1451 0.76 +1.2 1,673,050 - +0.7 1,673,472 - +0.3 1,673,663 + Shew_1452 0.14 +1.4 1,673,778 - Shew_1452 0.22 +0.8 1,673,922 + Shew_1452 0.32 +0.4 1,674,090 + Shew_1452 0.43 -0.5 1,674,339 + Shew_1452 0.59 +1.1 1,674,366 - Shew_1452 0.61 +0.6 1,674,366 - Shew_1452 0.61 +1.2 1,674,795 - Shew_1452 0.89 +1.5 1,674,945 - +0.3 1,675,256 - Shew_1453 0.40 +0.8 1,675,321 - Shew_1453 0.51 +0.9 1,675,414 - Shew_1453 0.67 +1.4 1,675,761 - +2.4
Or see this region's nucleotide sequence