Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1247 and Shew_1248 are separated by 9 nucleotides Shew_1248 and Shew_1249 overlap by 1 nucleotides Shew_1249 and Shew_1250 are separated by 452 nucleotides
Shew_1247: Shew_1247 - hypothetical protein (RefSeq), at 1,445,248 to 1,446,180
_1247
Shew_1248: Shew_1248 - phosphoribosylaminoimidazole carboxylase, catalytic subunit (RefSeq), at 1,446,190 to 1,446,672
_1248
Shew_1249: Shew_1249 - phosphoribosylaminoimidazole carboxylase, ATPase subunit (RefSeq), at 1,446,672 to 1,447,769
_1249
Shew_1250: Shew_1250 - hypothetical protein (RefSeq), at 1,448,222 to 1,448,908
_1250
Position (kb)
1446
1447
1448 Strain fitness (log2 ratio)
-2
-1
0
1 at 1445.780 kb on - strand, within Shew_1247 at 1445.820 kb on + strand, within Shew_1247 at 1447.104 kb on + strand, within Shew_1249 at 1447.221 kb on - strand, within Shew_1249 at 1447.400 kb on - strand, within Shew_1249 at 1447.404 kb on + strand, within Shew_1249 at 1447.419 kb on - strand, within Shew_1249 at 1447.797 kb on + strand at 1447.958 kb on + strand at 1448.029 kb on - strand at 1448.236 kb on + strand at 1448.401 kb on + strand, within Shew_1250 at 1448.409 kb on - strand, within Shew_1250 at 1448.442 kb on + strand, within Shew_1250 at 1448.563 kb on - strand, within Shew_1250 at 1448.570 kb on - strand, within Shew_1250 at 1448.615 kb on - strand, within Shew_1250 at 1448.642 kb on + strand, within Shew_1250 at 1448.700 kb on - strand, within Shew_1250 at 1448.762 kb on - strand, within Shew_1250
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 1,445,780 - Shew_1247 0.57 -1.4 1,445,820 + Shew_1247 0.61 +0.3 1,447,104 + Shew_1249 0.39 +1.5 1,447,221 - Shew_1249 0.50 +0.3 1,447,400 - Shew_1249 0.66 +0.3 1,447,404 + Shew_1249 0.67 +0.3 1,447,419 - Shew_1249 0.68 +0.8 1,447,797 + +0.8 1,447,958 + +1.6 1,448,029 - +0.6 1,448,236 + +1.1 1,448,401 + Shew_1250 0.26 -2.7 1,448,409 - Shew_1250 0.27 +0.2 1,448,442 + Shew_1250 0.32 +0.1 1,448,563 - Shew_1250 0.50 -0.4 1,448,570 - Shew_1250 0.51 +0.2 1,448,615 - Shew_1250 0.57 +0.9 1,448,642 + Shew_1250 0.61 +0.3 1,448,700 - Shew_1250 0.70 +0.8 1,448,762 - Shew_1250 0.79 -0.0
Or see this region's nucleotide sequence