Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhtA and Synpcc7942_2161 are separated by 12 nucleotides Synpcc7942_2161 and Synpcc7942_2162 are separated by 188 nucleotides Synpcc7942_2162 and Synpcc7942_2163 are separated by 96 nucleotides
Synpcc7942_2160: nhtA - alanine-glyoxylate aminotransferase, at 2,242,713 to 2,243,867
nhtA
Synpcc7942_2161: Synpcc7942_2161 - hypothetical protein, at 2,243,880 to 2,244,536
_2161
Synpcc7942_2162: Synpcc7942_2162 - hypothetical protein, at 2,244,725 to 2,244,940
_2162
Synpcc7942_2163: Synpcc7942_2163 - hypothetical protein, at 2,245,037 to 2,245,525
_2163
Position (kb)
2243
2244
2245 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2242.901 kb on - strand, within nhtA at 2242.971 kb on - strand at 2242.993 kb on - strand, within nhtA at 2243.097 kb on - strand, within nhtA at 2243.120 kb on - strand, within nhtA at 2243.153 kb on - strand, within nhtA at 2243.202 kb on + strand, within nhtA at 2243.210 kb on - strand, within nhtA at 2243.462 kb on + strand, within nhtA at 2243.669 kb on - strand, within nhtA at 2243.855 kb on + strand at 2243.855 kb on - strand at 2243.869 kb on - strand at 2243.871 kb on + strand at 2243.937 kb on - strand at 2243.949 kb on - strand, within Synpcc7942_2161 at 2243.985 kb on + strand, within Synpcc7942_2161 at 2244.069 kb on - strand, within Synpcc7942_2161 at 2244.196 kb on - strand, within Synpcc7942_2161 at 2244.201 kb on - strand, within Synpcc7942_2161 at 2244.201 kb on - strand, within Synpcc7942_2161 at 2244.201 kb on - strand, within Synpcc7942_2161 at 2244.210 kb on + strand, within Synpcc7942_2161 at 2244.395 kb on + strand, within Synpcc7942_2161 at 2244.431 kb on + strand, within Synpcc7942_2161 at 2244.431 kb on + strand, within Synpcc7942_2161 at 2244.431 kb on + strand, within Synpcc7942_2161 at 2244.478 kb on + strand at 2244.682 kb on - strand at 2244.682 kb on - strand at 2244.716 kb on + strand at 2244.740 kb on + strand at 2244.764 kb on - strand, within Synpcc7942_2162 at 2244.787 kb on - strand, within Synpcc7942_2162 at 2244.804 kb on - strand, within Synpcc7942_2162 at 2244.836 kb on + strand, within Synpcc7942_2162 at 2244.839 kb on - strand, within Synpcc7942_2162 at 2244.839 kb on - strand, within Synpcc7942_2162 at 2244.844 kb on - strand, within Synpcc7942_2162 at 2244.875 kb on - strand, within Synpcc7942_2162 at 2244.923 kb on - strand at 2244.923 kb on - strand at 2244.925 kb on + strand at 2244.925 kb on + strand at 2244.967 kb on + strand at 2244.975 kb on - strand at 2245.000 kb on + strand at 2245.033 kb on + strand at 2245.033 kb on + strand at 2245.033 kb on + strand at 2245.040 kb on + strand at 2245.041 kb on - strand at 2245.078 kb on + strand at 2245.092 kb on - strand, within Synpcc7942_2163 at 2245.125 kb on - strand, within Synpcc7942_2163 at 2245.139 kb on + strand, within Synpcc7942_2163 at 2245.140 kb on - strand, within Synpcc7942_2163 at 2245.147 kb on - strand, within Synpcc7942_2163 at 2245.147 kb on - strand, within Synpcc7942_2163 at 2245.163 kb on - strand, within Synpcc7942_2163 at 2245.185 kb on + strand, within Synpcc7942_2163 at 2245.210 kb on + strand, within Synpcc7942_2163 at 2245.266 kb on + strand, within Synpcc7942_2163 at 2245.295 kb on + strand, within Synpcc7942_2163 at 2245.308 kb on - strand at 2245.318 kb on - strand, within Synpcc7942_2163 at 2245.319 kb on - strand, within Synpcc7942_2163 at 2245.326 kb on - strand, within Synpcc7942_2163 at 2245.329 kb on - strand, within Synpcc7942_2163 at 2245.346 kb on - strand, within Synpcc7942_2163 at 2245.383 kb on + strand, within Synpcc7942_2163 at 2245.393 kb on + strand, within Synpcc7942_2163 at 2245.393 kb on + strand, within Synpcc7942_2163 at 2245.393 kb on + strand, within Synpcc7942_2163 at 2245.393 kb on + strand, within Synpcc7942_2163 at 2245.401 kb on - strand, within Synpcc7942_2163 at 2245.401 kb on - strand, within Synpcc7942_2163 at 2245.401 kb on - strand, within Synpcc7942_2163 at 2245.401 kb on - strand, within Synpcc7942_2163 at 2245.401 kb on - strand, within Synpcc7942_2163 at 2245.410 kb on + strand, within Synpcc7942_2163 at 2245.421 kb on - strand, within Synpcc7942_2163 at 2245.423 kb on + strand, within Synpcc7942_2163 at 2245.453 kb on + strand, within Synpcc7942_2163 at 2245.516 kb on - strand at 2245.520 kb on + strand at 2245.530 kb on + strand at 2245.530 kb on + strand at 2245.530 kb on + strand at 2245.530 kb on + strand at 2245.530 kb on + strand at 2245.530 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,242,901 - nhtA Synpcc7942_2160 0.16 +4.1 2,242,971 - +3.3 2,242,993 - nhtA Synpcc7942_2160 0.24 -1.1 2,243,097 - nhtA Synpcc7942_2160 0.33 +4.1 2,243,120 - nhtA Synpcc7942_2160 0.35 +3.4 2,243,153 - nhtA Synpcc7942_2160 0.38 -2.1 2,243,202 + nhtA Synpcc7942_2160 0.42 +2.6 2,243,210 - nhtA Synpcc7942_2160 0.43 -0.8 2,243,462 + nhtA Synpcc7942_2160 0.65 +3.5 2,243,669 - nhtA Synpcc7942_2160 0.83 +3.6 2,243,855 + +2.3 2,243,855 - +0.4 2,243,869 - -1.3 2,243,871 + +0.6 2,243,937 - +0.5 2,243,949 - Synpcc7942_2161 0.11 -1.9 2,243,985 + Synpcc7942_2161 0.16 -0.9 2,244,069 - Synpcc7942_2161 0.29 +0.1 2,244,196 - Synpcc7942_2161 0.48 +0.8 2,244,201 - Synpcc7942_2161 0.49 -1.2 2,244,201 - Synpcc7942_2161 0.49 -0.3 2,244,201 - Synpcc7942_2161 0.49 +2.4 2,244,210 + Synpcc7942_2161 0.50 -0.5 2,244,395 + Synpcc7942_2161 0.78 -0.3 2,244,431 + Synpcc7942_2161 0.84 +1.0 2,244,431 + Synpcc7942_2161 0.84 +0.0 2,244,431 + Synpcc7942_2161 0.84 +2.2 2,244,478 + +0.4 2,244,682 - +1.9 2,244,682 - -0.7 2,244,716 + -0.8 2,244,740 + +0.1 2,244,764 - Synpcc7942_2162 0.18 -0.7 2,244,787 - Synpcc7942_2162 0.29 +2.2 2,244,804 - Synpcc7942_2162 0.37 -1.1 2,244,836 + Synpcc7942_2162 0.51 +0.4 2,244,839 - Synpcc7942_2162 0.53 -1.7 2,244,839 - Synpcc7942_2162 0.53 +1.1 2,244,844 - Synpcc7942_2162 0.55 +2.7 2,244,875 - Synpcc7942_2162 0.69 -1.3 2,244,923 - -0.9 2,244,923 - +2.1 2,244,925 + -0.6 2,244,925 + +0.1 2,244,967 + -1.4 2,244,975 - +2.9 2,245,000 + -1.5 2,245,033 + -2.4 2,245,033 + +2.7 2,245,033 + +0.3 2,245,040 + +0.3 2,245,041 - -0.6 2,245,078 + -1.6 2,245,092 - Synpcc7942_2163 0.11 +0.4 2,245,125 - Synpcc7942_2163 0.18 -1.6 2,245,139 + Synpcc7942_2163 0.21 +3.8 2,245,140 - Synpcc7942_2163 0.21 -1.0 2,245,147 - Synpcc7942_2163 0.22 +0.9 2,245,147 - Synpcc7942_2163 0.22 -2.1 2,245,163 - Synpcc7942_2163 0.26 -0.5 2,245,185 + Synpcc7942_2163 0.30 -2.1 2,245,210 + Synpcc7942_2163 0.35 +1.4 2,245,266 + Synpcc7942_2163 0.47 -1.0 2,245,295 + Synpcc7942_2163 0.53 +0.0 2,245,308 - +2.0 2,245,318 - Synpcc7942_2163 0.57 +0.4 2,245,319 - Synpcc7942_2163 0.58 +1.9 2,245,326 - Synpcc7942_2163 0.59 +4.1 2,245,329 - Synpcc7942_2163 0.60 -0.6 2,245,346 - Synpcc7942_2163 0.63 -2.4 2,245,383 + Synpcc7942_2163 0.71 -0.3 2,245,393 + Synpcc7942_2163 0.73 -1.7 2,245,393 + Synpcc7942_2163 0.73 -0.8 2,245,393 + Synpcc7942_2163 0.73 -0.4 2,245,393 + Synpcc7942_2163 0.73 +1.2 2,245,401 - Synpcc7942_2163 0.74 +0.5 2,245,401 - Synpcc7942_2163 0.74 -0.1 2,245,401 - Synpcc7942_2163 0.74 +0.1 2,245,401 - Synpcc7942_2163 0.74 -0.8 2,245,401 - Synpcc7942_2163 0.74 +0.8 2,245,410 + Synpcc7942_2163 0.76 -0.5 2,245,421 - Synpcc7942_2163 0.79 +0.3 2,245,423 + Synpcc7942_2163 0.79 +3.3 2,245,453 + Synpcc7942_2163 0.85 -2.4 2,245,516 - +2.6 2,245,520 + +1.1 2,245,530 + -0.8 2,245,530 + +1.0 2,245,530 + -1.4 2,245,530 + -0.5 2,245,530 + -0.7 2,245,530 + -2.4
Or see this region's nucleotide sequence