Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mviN and Synpcc7942_2042 are separated by 6 nucleotides Synpcc7942_2042 and speH are separated by 116 nucleotides speH and Synpcc7942_2044 are separated by 304 nucleotides
Synpcc7942_2041: mviN - integral membrane protein MviN, at 2,110,181 to 2,111,803
mviN
Synpcc7942_2042: Synpcc7942_2042 - hypothetical protein, at 2,111,810 to 2,112,136
_2042
Synpcc7942_2043: speH - S-adenosylmethionine decarboxylase proenzyme, at 2,112,253 to 2,112,672
speH
Synpcc7942_2044: Synpcc7942_2044 - hypothetical protein, at 2,112,977 to 2,113,990
_2044
Position (kb)
2112
2113 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2111.797 kb on + strand at 2111.797 kb on + strand at 2111.797 kb on + strand at 2111.797 kb on + strand at 2111.802 kb on + strand at 2111.805 kb on - strand at 2111.805 kb on - strand at 2111.805 kb on - strand at 2111.805 kb on - strand at 2111.805 kb on - strand at 2111.805 kb on - strand at 2111.805 kb on - strand at 2111.805 kb on - strand at 2111.805 kb on - strand at 2111.807 kb on + strand at 2111.810 kb on - strand at 2111.820 kb on + strand at 2111.828 kb on - strand at 2111.830 kb on + strand at 2111.835 kb on - strand at 2111.888 kb on - strand, within Synpcc7942_2042 at 2111.931 kb on - strand, within Synpcc7942_2042 at 2111.947 kb on + strand, within Synpcc7942_2042 at 2111.947 kb on + strand, within Synpcc7942_2042 at 2112.005 kb on + strand, within Synpcc7942_2042 at 2112.014 kb on + strand, within Synpcc7942_2042 at 2112.017 kb on + strand, within Synpcc7942_2042 at 2112.024 kb on + strand, within Synpcc7942_2042 at 2112.024 kb on + strand, within Synpcc7942_2042 at 2112.024 kb on + strand, within Synpcc7942_2042 at 2112.030 kb on + strand, within Synpcc7942_2042 at 2112.030 kb on + strand, within Synpcc7942_2042 at 2112.030 kb on + strand, within Synpcc7942_2042 at 2112.030 kb on + strand, within Synpcc7942_2042 at 2112.030 kb on + strand, within Synpcc7942_2042 at 2112.031 kb on - strand, within Synpcc7942_2042 at 2112.032 kb on - strand, within Synpcc7942_2042 at 2112.032 kb on - strand, within Synpcc7942_2042 at 2112.038 kb on - strand, within Synpcc7942_2042 at 2112.038 kb on - strand, within Synpcc7942_2042 at 2112.038 kb on - strand, within Synpcc7942_2042 at 2112.038 kb on - strand, within Synpcc7942_2042 at 2112.038 kb on - strand at 2112.038 kb on - strand, within Synpcc7942_2042 at 2112.050 kb on - strand, within Synpcc7942_2042 at 2112.050 kb on - strand, within Synpcc7942_2042 at 2112.060 kb on + strand, within Synpcc7942_2042 at 2112.068 kb on - strand, within Synpcc7942_2042 at 2112.140 kb on + strand at 2112.148 kb on + strand at 2112.192 kb on - strand at 2112.214 kb on - strand at 2112.256 kb on + strand at 2112.295 kb on - strand, within speH at 2112.308 kb on - strand, within speH at 2112.322 kb on + strand, within speH at 2112.338 kb on - strand, within speH at 2112.388 kb on + strand, within speH at 2112.388 kb on + strand, within speH at 2112.388 kb on + strand, within speH at 2112.396 kb on - strand, within speH at 2112.396 kb on - strand, within speH at 2112.460 kb on + strand, within speH at 2112.460 kb on + strand, within speH at 2112.460 kb on + strand, within speH at 2112.468 kb on - strand, within speH at 2112.468 kb on - strand, within speH at 2112.490 kb on - strand, within speH at 2112.561 kb on + strand, within speH at 2112.564 kb on - strand, within speH at 2112.574 kb on - strand, within speH at 2112.577 kb on - strand, within speH at 2112.579 kb on - strand, within speH at 2112.579 kb on - strand at 2112.613 kb on + strand, within speH at 2112.618 kb on + strand, within speH at 2112.626 kb on - strand, within speH at 2112.626 kb on - strand, within speH at 2112.653 kb on + strand at 2112.767 kb on + strand at 2112.770 kb on - strand at 2112.770 kb on - strand at 2112.786 kb on - strand at 2112.833 kb on + strand at 2112.841 kb on - strand at 2112.880 kb on - strand at 2112.942 kb on - strand at 2112.965 kb on + strand at 2112.965 kb on + strand at 2112.965 kb on + strand at 2112.973 kb on - strand at 2112.973 kb on - strand at 2112.973 kb on - strand at 2112.983 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,111,797 + +0.1 2,111,797 + +2.8 2,111,797 + -1.5 2,111,797 + +1.7 2,111,802 + +0.0 2,111,805 - +2.7 2,111,805 - -1.3 2,111,805 - +0.2 2,111,805 - -0.4 2,111,805 - -1.2 2,111,805 - -0.2 2,111,805 - +0.6 2,111,805 - -0.2 2,111,805 - +1.4 2,111,807 + +0.6 2,111,810 - -0.5 2,111,820 + -1.2 2,111,828 - -0.5 2,111,830 + -3.2 2,111,835 - -4.1 2,111,888 - Synpcc7942_2042 0.24 -1.4 2,111,931 - Synpcc7942_2042 0.37 -3.3 2,111,947 + Synpcc7942_2042 0.42 -0.7 2,111,947 + Synpcc7942_2042 0.42 -2.1 2,112,005 + Synpcc7942_2042 0.60 -3.2 2,112,014 + Synpcc7942_2042 0.62 -4.5 2,112,017 + Synpcc7942_2042 0.63 +0.7 2,112,024 + Synpcc7942_2042 0.65 -2.4 2,112,024 + Synpcc7942_2042 0.65 -1.2 2,112,024 + Synpcc7942_2042 0.65 -1.8 2,112,030 + Synpcc7942_2042 0.67 -1.1 2,112,030 + Synpcc7942_2042 0.67 -2.4 2,112,030 + Synpcc7942_2042 0.67 -3.5 2,112,030 + Synpcc7942_2042 0.67 -1.3 2,112,030 + Synpcc7942_2042 0.67 -3.0 2,112,031 - Synpcc7942_2042 0.68 -4.7 2,112,032 - Synpcc7942_2042 0.68 -1.6 2,112,032 - Synpcc7942_2042 0.68 -3.6 2,112,038 - Synpcc7942_2042 0.70 -3.2 2,112,038 - Synpcc7942_2042 0.70 -4.4 2,112,038 - Synpcc7942_2042 0.70 -4.2 2,112,038 - Synpcc7942_2042 0.70 +0.7 2,112,038 - +1.1 2,112,038 - Synpcc7942_2042 0.70 -2.8 2,112,050 - Synpcc7942_2042 0.73 -3.1 2,112,050 - Synpcc7942_2042 0.73 -1.4 2,112,060 + Synpcc7942_2042 0.76 -5.0 2,112,068 - Synpcc7942_2042 0.79 -4.3 2,112,140 + +3.8 2,112,148 + -0.7 2,112,192 - -0.8 2,112,214 - +0.0 2,112,256 + +0.4 2,112,295 - speH Synpcc7942_2043 0.10 -3.2 2,112,308 - speH Synpcc7942_2043 0.13 +1.4 2,112,322 + speH Synpcc7942_2043 0.16 +4.4 2,112,338 - speH Synpcc7942_2043 0.20 -0.5 2,112,388 + speH Synpcc7942_2043 0.32 +2.6 2,112,388 + speH Synpcc7942_2043 0.32 +1.5 2,112,388 + speH Synpcc7942_2043 0.32 +0.4 2,112,396 - speH Synpcc7942_2043 0.34 +1.1 2,112,396 - speH Synpcc7942_2043 0.34 +2.1 2,112,460 + speH Synpcc7942_2043 0.49 +1.9 2,112,460 + speH Synpcc7942_2043 0.49 -1.3 2,112,460 + speH Synpcc7942_2043 0.49 -0.9 2,112,468 - speH Synpcc7942_2043 0.51 +0.9 2,112,468 - speH Synpcc7942_2043 0.51 -0.2 2,112,490 - speH Synpcc7942_2043 0.56 +1.5 2,112,561 + speH Synpcc7942_2043 0.73 -0.7 2,112,564 - speH Synpcc7942_2043 0.74 +1.6 2,112,574 - speH Synpcc7942_2043 0.76 +0.5 2,112,577 - speH Synpcc7942_2043 0.77 +2.0 2,112,579 - speH Synpcc7942_2043 0.78 -1.6 2,112,579 - +1.5 2,112,613 + speH Synpcc7942_2043 0.86 -0.1 2,112,618 + speH Synpcc7942_2043 0.87 -0.8 2,112,626 - speH Synpcc7942_2043 0.89 +2.2 2,112,626 - speH Synpcc7942_2043 0.89 +1.0 2,112,653 + -1.9 2,112,767 + -0.6 2,112,770 - -1.0 2,112,770 - -0.9 2,112,786 - +4.4 2,112,833 + -1.9 2,112,841 - -0.7 2,112,880 - +0.4 2,112,942 - -1.5 2,112,965 + +0.2 2,112,965 + -1.0 2,112,965 + +0.3 2,112,973 - -1.4 2,112,973 - +0.9 2,112,973 - +0.3 2,112,983 - +1.4
Or see this region's nucleotide sequence