Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1758 and Synpcc7942_1759 overlap by 19 nucleotides Synpcc7942_1759 and ald are separated by 75 nucleotides
Synpcc7942_1758: Synpcc7942_1758 - hypothetical protein, at 1,825,182 to 1,826,267
_1758
Synpcc7942_1759: Synpcc7942_1759 - CAB/ELIP/HLIP-related protein, at 1,826,249 to 1,826,452
_1759
Synpcc7942_1760: ald - L-alanine dehydrogenase, at 1,826,528 to 1,827,619
ald
Position (kb)
1826
1827 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 1825.249 kb on + strand at 1825.249 kb on + strand at 1825.250 kb on + strand at 1825.250 kb on + strand at 1825.250 kb on + strand at 1825.250 kb on + strand at 1825.250 kb on + strand at 1825.258 kb on - strand at 1825.258 kb on - strand at 1825.258 kb on - strand at 1825.258 kb on - strand at 1825.258 kb on - strand at 1825.279 kb on - strand at 1825.329 kb on - strand, within Synpcc7942_1758 at 1825.329 kb on - strand, within Synpcc7942_1758 at 1825.363 kb on + strand, within Synpcc7942_1758 at 1825.364 kb on - strand, within Synpcc7942_1758 at 1825.369 kb on + strand, within Synpcc7942_1758 at 1825.377 kb on - strand, within Synpcc7942_1758 at 1825.377 kb on - strand, within Synpcc7942_1758 at 1825.383 kb on + strand, within Synpcc7942_1758 at 1825.490 kb on - strand, within Synpcc7942_1758 at 1825.533 kb on - strand, within Synpcc7942_1758 at 1825.540 kb on - strand, within Synpcc7942_1758 at 1825.619 kb on - strand, within Synpcc7942_1758 at 1825.622 kb on + strand at 1825.681 kb on + strand, within Synpcc7942_1758 at 1825.691 kb on + strand, within Synpcc7942_1758 at 1825.691 kb on + strand, within Synpcc7942_1758 at 1825.699 kb on - strand, within Synpcc7942_1758 at 1825.746 kb on - strand, within Synpcc7942_1758 at 1825.761 kb on + strand, within Synpcc7942_1758 at 1825.764 kb on - strand, within Synpcc7942_1758 at 1825.778 kb on + strand, within Synpcc7942_1758 at 1825.814 kb on - strand, within Synpcc7942_1758 at 1825.816 kb on + strand, within Synpcc7942_1758 at 1825.816 kb on + strand, within Synpcc7942_1758 at 1825.816 kb on + strand, within Synpcc7942_1758 at 1825.816 kb on + strand, within Synpcc7942_1758 at 1825.852 kb on - strand, within Synpcc7942_1758 at 1825.875 kb on - strand, within Synpcc7942_1758 at 1825.908 kb on - strand, within Synpcc7942_1758 at 1825.913 kb on - strand, within Synpcc7942_1758 at 1825.913 kb on - strand, within Synpcc7942_1758 at 1825.946 kb on + strand, within Synpcc7942_1758 at 1825.954 kb on - strand, within Synpcc7942_1758 at 1825.961 kb on + strand, within Synpcc7942_1758 at 1826.013 kb on + strand, within Synpcc7942_1758 at 1826.021 kb on - strand at 1826.060 kb on + strand, within Synpcc7942_1758 at 1826.068 kb on - strand, within Synpcc7942_1758 at 1826.092 kb on + strand, within Synpcc7942_1758 at 1826.100 kb on - strand, within Synpcc7942_1758 at 1826.104 kb on + strand, within Synpcc7942_1758 at 1826.140 kb on - strand, within Synpcc7942_1758 at 1826.177 kb on + strand at 1826.182 kb on + strand at 1826.187 kb on + strand at 1826.195 kb on - strand at 1826.195 kb on - strand at 1826.206 kb on + strand at 1826.221 kb on + strand at 1826.221 kb on + strand at 1826.244 kb on - strand at 1826.246 kb on + strand at 1826.247 kb on + strand at 1826.251 kb on + strand at 1826.254 kb on - strand at 1826.254 kb on - strand at 1826.255 kb on - strand at 1826.276 kb on + strand, within Synpcc7942_1759 at 1826.367 kb on - strand, within Synpcc7942_1759 at 1826.398 kb on + strand, within Synpcc7942_1759 at 1826.401 kb on - strand, within Synpcc7942_1759 at 1826.404 kb on - strand, within Synpcc7942_1759 at 1826.406 kb on - strand, within Synpcc7942_1759 at 1826.406 kb on - strand, within Synpcc7942_1759 at 1826.446 kb on + strand at 1826.467 kb on + strand at 1826.507 kb on - strand at 1826.507 kb on - strand at 1826.541 kb on - strand at 1826.543 kb on - strand at 1826.576 kb on + strand at 1826.576 kb on + strand at 1826.576 kb on + strand at 1826.576 kb on + strand at 1826.584 kb on - strand at 1826.584 kb on - strand at 1826.594 kb on + strand at 1826.594 kb on - strand at 1826.599 kb on + strand at 1826.607 kb on - strand at 1826.609 kb on + strand at 1826.617 kb on - strand at 1826.631 kb on - strand at 1826.676 kb on + strand, within ald at 1826.688 kb on + strand, within ald at 1826.706 kb on + strand, within ald at 1826.711 kb on + strand, within ald at 1826.711 kb on + strand, within ald at 1826.730 kb on + strand, within ald at 1826.742 kb on + strand, within ald at 1826.742 kb on + strand, within ald at 1826.750 kb on - strand, within ald at 1826.755 kb on + strand, within ald at 1826.765 kb on + strand, within ald at 1826.765 kb on + strand, within ald at 1826.770 kb on - strand, within ald at 1826.786 kb on + strand, within ald at 1826.789 kb on - strand, within ald at 1826.794 kb on + strand, within ald at 1826.802 kb on - strand, within ald at 1826.804 kb on + strand, within ald at 1826.812 kb on - strand, within ald at 1826.827 kb on + strand, within ald at 1826.835 kb on - strand, within ald at 1826.843 kb on + strand, within ald at 1826.853 kb on - strand, within ald at 1826.900 kb on + strand, within ald at 1826.908 kb on - strand, within ald at 1827.018 kb on + strand, within ald at 1827.018 kb on + strand, within ald at 1827.041 kb on + strand, within ald at 1827.059 kb on - strand, within ald at 1827.063 kb on + strand, within ald at 1827.072 kb on - strand, within ald at 1827.125 kb on - strand, within ald at 1827.140 kb on + strand, within ald at 1827.142 kb on + strand, within ald at 1827.148 kb on - strand, within ald at 1827.162 kb on - strand, within ald at 1827.176 kb on + strand, within ald at 1827.176 kb on + strand, within ald at 1827.178 kb on - strand, within ald at 1827.184 kb on - strand, within ald at 1827.207 kb on + strand, within ald at 1827.207 kb on + strand, within ald at 1827.214 kb on - strand, within ald at 1827.217 kb on + strand, within ald at 1827.234 kb on + strand, within ald at 1827.237 kb on - strand, within ald at 1827.237 kb on - strand, within ald at 1827.276 kb on + strand, within ald at 1827.276 kb on + strand, within ald at 1827.276 kb on + strand, within ald at 1827.284 kb on - strand, within ald at 1827.284 kb on - strand, within ald at 1827.284 kb on - strand, within ald at 1827.296 kb on + strand, within ald at 1827.298 kb on - strand, within ald at 1827.303 kb on - strand, within ald at 1827.311 kb on + strand, within ald at 1827.314 kb on - strand, within ald at 1827.314 kb on - strand, within ald at 1827.319 kb on - strand, within ald at 1827.319 kb on - strand at 1827.347 kb on + strand, within ald at 1827.354 kb on + strand, within ald at 1827.354 kb on + strand, within ald at 1827.354 kb on + strand, within ald at 1827.355 kb on - strand, within ald at 1827.356 kb on - strand, within ald at 1827.359 kb on + strand, within ald at 1827.368 kb on + strand, within ald at 1827.368 kb on + strand, within ald at 1827.376 kb on - strand, within ald at 1827.376 kb on - strand, within ald at 1827.404 kb on + strand, within ald at 1827.439 kb on + strand, within ald at 1827.439 kb on + strand, within ald
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,825,249 + +0.3 1,825,249 + +0.7 1,825,250 + +4.5 1,825,250 + +1.1 1,825,250 + -0.6 1,825,250 + -0.4 1,825,250 + +1.1 1,825,258 - +0.6 1,825,258 - +1.0 1,825,258 - -0.2 1,825,258 - +1.0 1,825,258 - +0.8 1,825,279 - +1.2 1,825,329 - Synpcc7942_1758 0.14 +0.8 1,825,329 - Synpcc7942_1758 0.14 +0.6 1,825,363 + Synpcc7942_1758 0.17 +1.6 1,825,364 - Synpcc7942_1758 0.17 +0.8 1,825,369 + Synpcc7942_1758 0.17 -0.9 1,825,377 - Synpcc7942_1758 0.18 +2.3 1,825,377 - Synpcc7942_1758 0.18 +0.9 1,825,383 + Synpcc7942_1758 0.19 +0.9 1,825,490 - Synpcc7942_1758 0.28 +1.1 1,825,533 - Synpcc7942_1758 0.32 +0.7 1,825,540 - Synpcc7942_1758 0.33 -0.5 1,825,619 - Synpcc7942_1758 0.40 -0.2 1,825,622 + -1.5 1,825,681 + Synpcc7942_1758 0.46 -0.0 1,825,691 + Synpcc7942_1758 0.47 -0.5 1,825,691 + Synpcc7942_1758 0.47 +1.6 1,825,699 - Synpcc7942_1758 0.48 -0.4 1,825,746 - Synpcc7942_1758 0.52 -1.2 1,825,761 + Synpcc7942_1758 0.53 +0.4 1,825,764 - Synpcc7942_1758 0.54 +2.1 1,825,778 + Synpcc7942_1758 0.55 -4.0 1,825,814 - Synpcc7942_1758 0.58 -2.6 1,825,816 + Synpcc7942_1758 0.58 -0.7 1,825,816 + Synpcc7942_1758 0.58 -0.2 1,825,816 + Synpcc7942_1758 0.58 +0.6 1,825,816 + Synpcc7942_1758 0.58 -0.7 1,825,852 - Synpcc7942_1758 0.62 +1.1 1,825,875 - Synpcc7942_1758 0.64 +4.6 1,825,908 - Synpcc7942_1758 0.67 +1.5 1,825,913 - Synpcc7942_1758 0.67 +1.1 1,825,913 - Synpcc7942_1758 0.67 +0.8 1,825,946 + Synpcc7942_1758 0.70 +0.4 1,825,954 - Synpcc7942_1758 0.71 -0.2 1,825,961 + Synpcc7942_1758 0.72 +0.4 1,826,013 + Synpcc7942_1758 0.77 +0.9 1,826,021 - +3.9 1,826,060 + Synpcc7942_1758 0.81 +1.9 1,826,068 - Synpcc7942_1758 0.82 +4.4 1,826,092 + Synpcc7942_1758 0.84 -0.1 1,826,100 - Synpcc7942_1758 0.85 +0.2 1,826,104 + Synpcc7942_1758 0.85 -0.4 1,826,140 - Synpcc7942_1758 0.88 +1.1 1,826,177 + -0.9 1,826,182 + -0.3 1,826,187 + +1.8 1,826,195 - +1.6 1,826,195 - +0.9 1,826,206 + -0.4 1,826,221 + -1.1 1,826,221 + +3.3 1,826,244 - +3.4 1,826,246 + -0.9 1,826,247 + +0.4 1,826,251 + -0.8 1,826,254 - +4.0 1,826,254 - +1.4 1,826,255 - +0.9 1,826,276 + Synpcc7942_1759 0.13 +4.6 1,826,367 - Synpcc7942_1759 0.58 +1.1 1,826,398 + Synpcc7942_1759 0.73 -0.3 1,826,401 - Synpcc7942_1759 0.75 +2.2 1,826,404 - Synpcc7942_1759 0.76 -1.6 1,826,406 - Synpcc7942_1759 0.77 +0.5 1,826,406 - Synpcc7942_1759 0.77 +1.7 1,826,446 + +1.3 1,826,467 + +1.1 1,826,507 - +5.7 1,826,507 - +5.5 1,826,541 - +5.8 1,826,543 - +3.4 1,826,576 + +6.2 1,826,576 + +7.2 1,826,576 + +6.3 1,826,576 + +5.4 1,826,584 - +3.3 1,826,584 - +5.2 1,826,594 + +6.6 1,826,594 - +7.2 1,826,599 + +5.1 1,826,607 - +2.7 1,826,609 + +6.6 1,826,617 - +6.1 1,826,631 - +6.0 1,826,676 + ald Synpcc7942_1760 0.14 +6.2 1,826,688 + ald Synpcc7942_1760 0.15 +6.2 1,826,706 + ald Synpcc7942_1760 0.16 +4.3 1,826,711 + ald Synpcc7942_1760 0.17 +5.8 1,826,711 + ald Synpcc7942_1760 0.17 +4.0 1,826,730 + ald Synpcc7942_1760 0.18 +5.8 1,826,742 + ald Synpcc7942_1760 0.20 +6.9 1,826,742 + ald Synpcc7942_1760 0.20 +3.1 1,826,750 - ald Synpcc7942_1760 0.20 +5.2 1,826,755 + ald Synpcc7942_1760 0.21 +5.6 1,826,765 + ald Synpcc7942_1760 0.22 +2.2 1,826,765 + ald Synpcc7942_1760 0.22 +6.1 1,826,770 - ald Synpcc7942_1760 0.22 +6.3 1,826,786 + ald Synpcc7942_1760 0.24 +6.1 1,826,789 - ald Synpcc7942_1760 0.24 +2.1 1,826,794 + ald Synpcc7942_1760 0.24 +3.2 1,826,802 - ald Synpcc7942_1760 0.25 +3.2 1,826,804 + ald Synpcc7942_1760 0.25 +5.8 1,826,812 - ald Synpcc7942_1760 0.26 +6.3 1,826,827 + ald Synpcc7942_1760 0.27 +3.5 1,826,835 - ald Synpcc7942_1760 0.28 +4.3 1,826,843 + ald Synpcc7942_1760 0.29 +4.5 1,826,853 - ald Synpcc7942_1760 0.30 +5.6 1,826,900 + ald Synpcc7942_1760 0.34 +3.4 1,826,908 - ald Synpcc7942_1760 0.35 +3.1 1,827,018 + ald Synpcc7942_1760 0.45 +6.9 1,827,018 + ald Synpcc7942_1760 0.45 +5.9 1,827,041 + ald Synpcc7942_1760 0.47 +6.4 1,827,059 - ald Synpcc7942_1760 0.49 +3.2 1,827,063 + ald Synpcc7942_1760 0.49 +4.6 1,827,072 - ald Synpcc7942_1760 0.50 +2.4 1,827,125 - ald Synpcc7942_1760 0.55 +5.3 1,827,140 + ald Synpcc7942_1760 0.56 +5.7 1,827,142 + ald Synpcc7942_1760 0.56 +3.4 1,827,148 - ald Synpcc7942_1760 0.57 +5.2 1,827,162 - ald Synpcc7942_1760 0.58 +6.2 1,827,176 + ald Synpcc7942_1760 0.59 +3.0 1,827,176 + ald Synpcc7942_1760 0.59 +4.9 1,827,178 - ald Synpcc7942_1760 0.60 +5.1 1,827,184 - ald Synpcc7942_1760 0.60 +4.8 1,827,207 + ald Synpcc7942_1760 0.62 +5.4 1,827,207 + ald Synpcc7942_1760 0.62 +6.1 1,827,214 - ald Synpcc7942_1760 0.63 +3.6 1,827,217 + ald Synpcc7942_1760 0.63 +5.4 1,827,234 + ald Synpcc7942_1760 0.65 +4.2 1,827,237 - ald Synpcc7942_1760 0.65 +3.5 1,827,237 - ald Synpcc7942_1760 0.65 +5.5 1,827,276 + ald Synpcc7942_1760 0.68 +3.9 1,827,276 + ald Synpcc7942_1760 0.68 +5.9 1,827,276 + ald Synpcc7942_1760 0.68 +4.6 1,827,284 - ald Synpcc7942_1760 0.69 +5.4 1,827,284 - ald Synpcc7942_1760 0.69 +4.1 1,827,284 - ald Synpcc7942_1760 0.69 +4.7 1,827,296 + ald Synpcc7942_1760 0.70 +5.4 1,827,298 - ald Synpcc7942_1760 0.71 +6.3 1,827,303 - ald Synpcc7942_1760 0.71 +4.5 1,827,311 + ald Synpcc7942_1760 0.72 +5.6 1,827,314 - ald Synpcc7942_1760 0.72 +6.1 1,827,314 - ald Synpcc7942_1760 0.72 +4.8 1,827,319 - ald Synpcc7942_1760 0.72 +6.0 1,827,319 - +6.2 1,827,347 + ald Synpcc7942_1760 0.75 +7.4 1,827,354 + ald Synpcc7942_1760 0.76 +2.8 1,827,354 + ald Synpcc7942_1760 0.76 +2.9 1,827,354 + ald Synpcc7942_1760 0.76 +2.8 1,827,355 - ald Synpcc7942_1760 0.76 +5.3 1,827,356 - ald Synpcc7942_1760 0.76 +3.3 1,827,359 + ald Synpcc7942_1760 0.76 +5.3 1,827,368 + ald Synpcc7942_1760 0.77 +4.7 1,827,368 + ald Synpcc7942_1760 0.77 +5.1 1,827,376 - ald Synpcc7942_1760 0.78 +5.9 1,827,376 - ald Synpcc7942_1760 0.78 +6.0 1,827,404 + ald Synpcc7942_1760 0.80 +5.6 1,827,439 + ald Synpcc7942_1760 0.83 +2.1 1,827,439 + ald Synpcc7942_1760 0.83 +5.4
Or see this region's nucleotide sequence