Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1381 and Synpcc7942_1382 are separated by 431 nucleotides Synpcc7942_1382 and ppa are separated by 86 nucleotides ppa and Synpcc7942_1384 are separated by 127 nucleotides
Synpcc7942_1381: Synpcc7942_1381 - ATPase, at 1,424,685 to 1,426,610
_1381
Synpcc7942_1382: Synpcc7942_1382 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 1,427,042 to 1,429,711
_1382
Synpcc7942_1383: ppa - Inorganic diphosphatase, at 1,429,798 to 1,430,310
ppa
Synpcc7942_1384: Synpcc7942_1384 - hypothetical protein, at 1,430,438 to 1,431,283
_1384
Position (kb)
1427
1428
1429
1430 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1426.054 kb on - strand, within Synpcc7942_1381 at 1426.204 kb on + strand, within Synpcc7942_1381 at 1426.253 kb on + strand, within Synpcc7942_1381 at 1426.481 kb on - strand at 1426.481 kb on - strand at 1426.492 kb on + strand at 1426.539 kb on + strand at 1426.586 kb on - strand at 1426.605 kb on + strand at 1426.691 kb on + strand at 1426.704 kb on - strand at 1426.704 kb on - strand at 1426.704 kb on - strand at 1426.704 kb on - strand at 1426.704 kb on - strand at 1426.704 kb on - strand at 1426.704 kb on - strand at 1426.704 kb on - strand at 1426.742 kb on + strand at 1426.753 kb on + strand at 1426.893 kb on - strand at 1426.934 kb on + strand at 1427.053 kb on - strand at 1427.063 kb on - strand at 1427.121 kb on - strand at 1427.184 kb on + strand at 1427.223 kb on + strand at 1427.224 kb on - strand at 1427.240 kb on + strand at 1427.243 kb on - strand at 1427.341 kb on + strand, within Synpcc7942_1382 at 1427.365 kb on + strand, within Synpcc7942_1382 at 1427.374 kb on - strand, within Synpcc7942_1382 at 1427.391 kb on + strand, within Synpcc7942_1382 at 1427.398 kb on - strand, within Synpcc7942_1382 at 1427.399 kb on - strand, within Synpcc7942_1382 at 1427.459 kb on + strand, within Synpcc7942_1382 at 1427.467 kb on - strand, within Synpcc7942_1382 at 1427.469 kb on + strand, within Synpcc7942_1382 at 1427.472 kb on - strand, within Synpcc7942_1382 at 1427.571 kb on + strand, within Synpcc7942_1382 at 1427.611 kb on + strand, within Synpcc7942_1382 at 1427.619 kb on - strand, within Synpcc7942_1382 at 1427.688 kb on - strand, within Synpcc7942_1382 at 1427.750 kb on + strand, within Synpcc7942_1382 at 1427.761 kb on + strand, within Synpcc7942_1382 at 1427.849 kb on - strand, within Synpcc7942_1382 at 1427.958 kb on - strand, within Synpcc7942_1382 at 1428.096 kb on + strand, within Synpcc7942_1382 at 1428.164 kb on + strand, within Synpcc7942_1382 at 1428.184 kb on + strand, within Synpcc7942_1382 at 1428.212 kb on - strand, within Synpcc7942_1382 at 1428.387 kb on + strand, within Synpcc7942_1382 at 1428.471 kb on - strand, within Synpcc7942_1382 at 1428.553 kb on + strand, within Synpcc7942_1382 at 1428.670 kb on + strand, within Synpcc7942_1382 at 1428.676 kb on - strand, within Synpcc7942_1382 at 1428.678 kb on - strand, within Synpcc7942_1382 at 1428.700 kb on + strand, within Synpcc7942_1382 at 1428.700 kb on + strand, within Synpcc7942_1382 at 1428.741 kb on - strand, within Synpcc7942_1382 at 1428.756 kb on - strand, within Synpcc7942_1382 at 1428.810 kb on + strand, within Synpcc7942_1382 at 1428.812 kb on - strand, within Synpcc7942_1382 at 1428.812 kb on - strand, within Synpcc7942_1382 at 1428.820 kb on + strand, within Synpcc7942_1382 at 1428.870 kb on - strand, within Synpcc7942_1382 at 1428.872 kb on + strand, within Synpcc7942_1382 at 1428.880 kb on + strand, within Synpcc7942_1382 at 1428.913 kb on - strand, within Synpcc7942_1382 at 1428.927 kb on + strand, within Synpcc7942_1382 at 1428.950 kb on + strand, within Synpcc7942_1382 at 1429.041 kb on + strand, within Synpcc7942_1382 at 1429.074 kb on - strand, within Synpcc7942_1382 at 1429.078 kb on + strand, within Synpcc7942_1382 at 1429.088 kb on + strand, within Synpcc7942_1382 at 1429.090 kb on - strand, within Synpcc7942_1382 at 1429.213 kb on - strand, within Synpcc7942_1382 at 1429.215 kb on - strand, within Synpcc7942_1382 at 1429.469 kb on - strand at 1429.539 kb on + strand at 1429.568 kb on - strand at 1429.648 kb on - strand at 1429.653 kb on - strand at 1429.709 kb on - strand at 1429.767 kb on - strand at 1429.769 kb on + strand at 1429.772 kb on - strand at 1429.772 kb on - strand at 1430.392 kb on - strand at 1430.438 kb on + strand at 1430.479 kb on + strand at 1430.487 kb on - strand at 1430.487 kb on - strand at 1430.510 kb on + strand at 1430.510 kb on - strand at 1430.530 kb on + strand, within Synpcc7942_1384 at 1430.538 kb on - strand, within Synpcc7942_1384 at 1430.541 kb on + strand, within Synpcc7942_1384 at 1430.541 kb on + strand, within Synpcc7942_1384 at 1430.543 kb on - strand, within Synpcc7942_1384 at 1430.549 kb on - strand, within Synpcc7942_1384 at 1430.549 kb on - strand, within Synpcc7942_1384 at 1430.556 kb on + strand, within Synpcc7942_1384 at 1430.557 kb on + strand, within Synpcc7942_1384 at 1430.559 kb on - strand, within Synpcc7942_1384 at 1430.571 kb on + strand, within Synpcc7942_1384 at 1430.627 kb on + strand, within Synpcc7942_1384 at 1430.637 kb on + strand, within Synpcc7942_1384 at 1430.637 kb on + strand, within Synpcc7942_1384 at 1430.660 kb on + strand, within Synpcc7942_1384 at 1430.678 kb on - strand, within Synpcc7942_1384 at 1430.686 kb on - strand, within Synpcc7942_1384
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,426,054 - Synpcc7942_1381 0.71 -2.2 1,426,204 + Synpcc7942_1381 0.79 -1.9 1,426,253 + Synpcc7942_1381 0.81 -2.4 1,426,481 - -1.6 1,426,481 - -0.4 1,426,492 + -2.0 1,426,539 + -0.0 1,426,586 - -0.5 1,426,605 + +3.4 1,426,691 + +2.1 1,426,704 - +0.3 1,426,704 - -0.6 1,426,704 - -0.5 1,426,704 - +0.2 1,426,704 - -1.0 1,426,704 - +0.4 1,426,704 - +2.8 1,426,704 - -0.1 1,426,742 + +0.5 1,426,753 + +0.7 1,426,893 - -3.0 1,426,934 + +0.6 1,427,053 - -2.6 1,427,063 - -0.7 1,427,121 - -2.1 1,427,184 + -2.4 1,427,223 + +1.4 1,427,224 - +0.3 1,427,240 + -0.3 1,427,243 - -3.3 1,427,341 + Synpcc7942_1382 0.11 -1.6 1,427,365 + Synpcc7942_1382 0.12 -2.0 1,427,374 - Synpcc7942_1382 0.12 -1.6 1,427,391 + Synpcc7942_1382 0.13 -1.0 1,427,398 - Synpcc7942_1382 0.13 -0.4 1,427,399 - Synpcc7942_1382 0.13 -0.8 1,427,459 + Synpcc7942_1382 0.16 -2.3 1,427,467 - Synpcc7942_1382 0.16 -1.9 1,427,469 + Synpcc7942_1382 0.16 -1.5 1,427,472 - Synpcc7942_1382 0.16 -1.5 1,427,571 + Synpcc7942_1382 0.20 -0.5 1,427,611 + Synpcc7942_1382 0.21 -1.1 1,427,619 - Synpcc7942_1382 0.22 -2.4 1,427,688 - Synpcc7942_1382 0.24 -0.7 1,427,750 + Synpcc7942_1382 0.27 +0.3 1,427,761 + Synpcc7942_1382 0.27 +0.9 1,427,849 - Synpcc7942_1382 0.30 -0.2 1,427,958 - Synpcc7942_1382 0.34 -0.5 1,428,096 + Synpcc7942_1382 0.39 -0.2 1,428,164 + Synpcc7942_1382 0.42 -2.5 1,428,184 + Synpcc7942_1382 0.43 +0.1 1,428,212 - Synpcc7942_1382 0.44 +0.2 1,428,387 + Synpcc7942_1382 0.50 -1.7 1,428,471 - Synpcc7942_1382 0.54 -1.6 1,428,553 + Synpcc7942_1382 0.57 -1.0 1,428,670 + Synpcc7942_1382 0.61 +0.1 1,428,676 - Synpcc7942_1382 0.61 -0.9 1,428,678 - Synpcc7942_1382 0.61 +0.1 1,428,700 + Synpcc7942_1382 0.62 -2.0 1,428,700 + Synpcc7942_1382 0.62 -0.9 1,428,741 - Synpcc7942_1382 0.64 -0.7 1,428,756 - Synpcc7942_1382 0.64 -0.5 1,428,810 + Synpcc7942_1382 0.66 +1.3 1,428,812 - Synpcc7942_1382 0.66 -1.2 1,428,812 - Synpcc7942_1382 0.66 -0.7 1,428,820 + Synpcc7942_1382 0.67 -0.8 1,428,870 - Synpcc7942_1382 0.68 +0.6 1,428,872 + Synpcc7942_1382 0.69 -2.2 1,428,880 + Synpcc7942_1382 0.69 -0.7 1,428,913 - Synpcc7942_1382 0.70 +3.4 1,428,927 + Synpcc7942_1382 0.71 -0.3 1,428,950 + Synpcc7942_1382 0.71 -1.3 1,429,041 + Synpcc7942_1382 0.75 -2.0 1,429,074 - Synpcc7942_1382 0.76 -2.2 1,429,078 + Synpcc7942_1382 0.76 -0.5 1,429,088 + Synpcc7942_1382 0.77 -1.6 1,429,090 - Synpcc7942_1382 0.77 +0.5 1,429,213 - Synpcc7942_1382 0.81 -0.9 1,429,215 - Synpcc7942_1382 0.81 -1.8 1,429,469 - +0.1 1,429,539 + -1.1 1,429,568 - -0.9 1,429,648 - +1.0 1,429,653 - +0.5 1,429,709 - +1.1 1,429,767 - -1.0 1,429,769 + +0.4 1,429,772 - +3.2 1,429,772 - -0.7 1,430,392 - -0.8 1,430,438 + -1.0 1,430,479 + +0.0 1,430,487 - +0.2 1,430,487 - +1.0 1,430,510 + -0.9 1,430,510 - -3.1 1,430,530 + Synpcc7942_1384 0.11 +0.7 1,430,538 - Synpcc7942_1384 0.12 +0.5 1,430,541 + Synpcc7942_1384 0.12 +0.4 1,430,541 + Synpcc7942_1384 0.12 -0.5 1,430,543 - Synpcc7942_1384 0.12 +1.5 1,430,549 - Synpcc7942_1384 0.13 +1.3 1,430,549 - Synpcc7942_1384 0.13 -0.4 1,430,556 + Synpcc7942_1384 0.14 -1.9 1,430,557 + Synpcc7942_1384 0.14 +0.1 1,430,559 - Synpcc7942_1384 0.14 -2.2 1,430,571 + Synpcc7942_1384 0.16 +1.0 1,430,627 + Synpcc7942_1384 0.22 +0.8 1,430,637 + Synpcc7942_1384 0.24 -0.9 1,430,637 + Synpcc7942_1384 0.24 +0.3 1,430,660 + Synpcc7942_1384 0.26 -1.0 1,430,678 - Synpcc7942_1384 0.28 +0.1 1,430,686 - Synpcc7942_1384 0.29 -0.6
Or see this region's nucleotide sequence