Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amt1 and Synpcc7942_0443 are separated by 124 nucleotides Synpcc7942_0443 and Synpcc7942_0444 are separated by 39 nucleotides
Synpcc7942_0442: amt1 - ammonium transporter, at 431,458 to 432,951
amt1
Synpcc7942_0443: Synpcc7942_0443 - hypothetical protein, at 433,076 to 434,317
_0443
Synpcc7942_0444: Synpcc7942_0444 - hypothetical protein, at 434,357 to 435,040
_0444
Position (kb) 433
434
435 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 432.100 kb on + strand, within amt1 at 432.140 kb on + strand, within amt1 at 432.203 kb on - strand, within amt1 at 432.217 kb on + strand, within amt1 at 432.222 kb on + strand, within amt1 at 432.225 kb on - strand, within amt1 at 432.225 kb on - strand, within amt1 at 432.230 kb on + strand, within amt1 at 432.242 kb on + strand, within amt1 at 432.266 kb on - strand, within amt1 at 432.297 kb on - strand, within amt1 at 432.297 kb on - strand, within amt1 at 432.297 kb on - strand, within amt1 at 432.359 kb on + strand, within amt1 at 432.378 kb on - strand, within amt1 at 432.404 kb on + strand, within amt1 at 432.404 kb on + strand, within amt1 at 432.412 kb on - strand, within amt1 at 432.412 kb on - strand, within amt1 at 432.412 kb on - strand, within amt1 at 432.414 kb on + strand, within amt1 at 432.435 kb on - strand, within amt1 at 432.457 kb on - strand, within amt1 at 432.469 kb on + strand, within amt1 at 432.477 kb on + strand, within amt1 at 432.516 kb on - strand, within amt1 at 432.538 kb on + strand, within amt1 at 432.609 kb on - strand, within amt1 at 432.696 kb on + strand, within amt1 at 432.717 kb on + strand, within amt1 at 432.717 kb on + strand, within amt1 at 432.717 kb on + strand, within amt1 at 432.725 kb on - strand, within amt1 at 432.725 kb on - strand, within amt1 at 432.727 kb on + strand, within amt1 at 432.738 kb on - strand, within amt1 at 432.777 kb on + strand, within amt1 at 432.795 kb on - strand, within amt1 at 432.824 kb on - strand at 432.864 kb on - strand at 432.865 kb on + strand at 432.873 kb on - strand at 432.907 kb on - strand at 432.928 kb on + strand at 432.952 kb on + strand at 433.054 kb on - strand at 433.057 kb on + strand at 433.103 kb on + strand at 433.181 kb on + strand at 433.181 kb on + strand at 433.186 kb on + strand at 433.189 kb on - strand at 433.189 kb on - strand at 433.189 kb on - strand at 433.189 kb on - strand at 433.194 kb on - strand at 433.204 kb on - strand, within Synpcc7942_0443 at 433.238 kb on - strand, within Synpcc7942_0443 at 433.239 kb on + strand, within Synpcc7942_0443 at 433.245 kb on - strand, within Synpcc7942_0443 at 433.265 kb on + strand at 433.270 kb on - strand, within Synpcc7942_0443 at 433.273 kb on - strand, within Synpcc7942_0443 at 433.281 kb on + strand, within Synpcc7942_0443 at 433.288 kb on - strand, within Synpcc7942_0443 at 433.288 kb on - strand, within Synpcc7942_0443 at 433.305 kb on - strand, within Synpcc7942_0443 at 433.307 kb on + strand, within Synpcc7942_0443 at 433.309 kb on + strand, within Synpcc7942_0443 at 433.315 kb on - strand, within Synpcc7942_0443 at 433.325 kb on - strand, within Synpcc7942_0443 at 433.346 kb on + strand, within Synpcc7942_0443 at 433.373 kb on + strand, within Synpcc7942_0443 at 433.376 kb on - strand, within Synpcc7942_0443 at 433.506 kb on + strand, within Synpcc7942_0443 at 433.512 kb on - strand, within Synpcc7942_0443 at 433.516 kb on + strand, within Synpcc7942_0443 at 433.594 kb on + strand, within Synpcc7942_0443 at 433.594 kb on + strand, within Synpcc7942_0443 at 433.602 kb on - strand, within Synpcc7942_0443 at 433.602 kb on - strand, within Synpcc7942_0443 at 433.603 kb on - strand, within Synpcc7942_0443 at 433.674 kb on + strand, within Synpcc7942_0443 at 433.680 kb on + strand, within Synpcc7942_0443 at 433.750 kb on + strand, within Synpcc7942_0443 at 433.763 kb on - strand, within Synpcc7942_0443 at 433.778 kb on - strand, within Synpcc7942_0443 at 433.788 kb on - strand at 433.811 kb on + strand, within Synpcc7942_0443 at 433.811 kb on + strand, within Synpcc7942_0443 at 433.876 kb on + strand, within Synpcc7942_0443 at 433.882 kb on + strand, within Synpcc7942_0443 at 433.890 kb on - strand, within Synpcc7942_0443 at 433.890 kb on - strand, within Synpcc7942_0443 at 433.918 kb on - strand, within Synpcc7942_0443 at 433.920 kb on - strand, within Synpcc7942_0443 at 433.957 kb on - strand, within Synpcc7942_0443 at 434.009 kb on - strand, within Synpcc7942_0443 at 434.104 kb on + strand, within Synpcc7942_0443 at 434.112 kb on - strand, within Synpcc7942_0443 at 434.153 kb on - strand, within Synpcc7942_0443 at 434.174 kb on + strand, within Synpcc7942_0443 at 434.182 kb on - strand, within Synpcc7942_0443 at 434.182 kb on - strand, within Synpcc7942_0443 at 434.182 kb on - strand, within Synpcc7942_0443 at 434.211 kb on + strand at 434.217 kb on + strand at 434.221 kb on + strand at 434.226 kb on - strand at 434.227 kb on + strand at 434.236 kb on + strand at 434.243 kb on - strand at 434.244 kb on - strand at 434.251 kb on - strand at 434.262 kb on - strand at 434.306 kb on + strand at 434.312 kb on + strand at 434.315 kb on + strand at 434.343 kb on + strand at 434.349 kb on + strand at 434.361 kb on - strand at 434.378 kb on + strand at 434.383 kb on + strand at 434.383 kb on + strand at 434.391 kb on - strand at 434.506 kb on - strand, within Synpcc7942_0444 at 434.516 kb on + strand, within Synpcc7942_0444 at 434.516 kb on + strand, within Synpcc7942_0444 at 434.516 kb on + strand, within Synpcc7942_0444 at 434.522 kb on - strand, within Synpcc7942_0444 at 434.523 kb on + strand, within Synpcc7942_0444 at 434.524 kb on - strand, within Synpcc7942_0444 at 434.524 kb on - strand, within Synpcc7942_0444 at 434.524 kb on - strand, within Synpcc7942_0444 at 434.526 kb on + strand, within Synpcc7942_0444 at 434.526 kb on + strand, within Synpcc7942_0444 at 434.528 kb on + strand, within Synpcc7942_0444 at 434.533 kb on + strand, within Synpcc7942_0444 at 434.534 kb on - strand, within Synpcc7942_0444 at 434.541 kb on - strand, within Synpcc7942_0444 at 434.567 kb on - strand, within Synpcc7942_0444 at 434.652 kb on + strand, within Synpcc7942_0444 at 434.660 kb on - strand, within Synpcc7942_0444 at 434.692 kb on - strand, within Synpcc7942_0444 at 434.731 kb on - strand, within Synpcc7942_0444 at 434.749 kb on + strand, within Synpcc7942_0444 at 434.754 kb on - strand, within Synpcc7942_0444 at 434.789 kb on + strand, within Synpcc7942_0444 at 434.846 kb on - strand, within Synpcc7942_0444 at 434.856 kb on - strand, within Synpcc7942_0444 at 434.875 kb on + strand, within Synpcc7942_0444 at 434.898 kb on + strand, within Synpcc7942_0444 at 434.948 kb on + strand, within Synpcc7942_0444 at 435.016 kb on + strand at 435.016 kb on + strand at 435.016 kb on + strand at 435.018 kb on + strand at 435.026 kb on - strand at 435.043 kb on - strand at 435.117 kb on - strand at 435.308 kb on - strand at 435.310 kb on + strand at 435.310 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 432,100 + amt1 Synpcc7942_0442 0.43 +0.1 432,140 + amt1 Synpcc7942_0442 0.46 -1.7 432,203 - amt1 Synpcc7942_0442 0.50 -1.2 432,217 + amt1 Synpcc7942_0442 0.51 -2.3 432,222 + amt1 Synpcc7942_0442 0.51 +0.8 432,225 - amt1 Synpcc7942_0442 0.51 -0.5 432,225 - amt1 Synpcc7942_0442 0.51 +0.2 432,230 + amt1 Synpcc7942_0442 0.52 -1.8 432,242 + amt1 Synpcc7942_0442 0.52 +0.9 432,266 - amt1 Synpcc7942_0442 0.54 -0.8 432,297 - amt1 Synpcc7942_0442 0.56 -1.3 432,297 - amt1 Synpcc7942_0442 0.56 +0.2 432,297 - amt1 Synpcc7942_0442 0.56 +0.7 432,359 + amt1 Synpcc7942_0442 0.60 -1.4 432,378 - amt1 Synpcc7942_0442 0.62 -2.7 432,404 + amt1 Synpcc7942_0442 0.63 +0.6 432,404 + amt1 Synpcc7942_0442 0.63 +1.0 432,412 - amt1 Synpcc7942_0442 0.64 +2.0 432,412 - amt1 Synpcc7942_0442 0.64 -0.1 432,412 - amt1 Synpcc7942_0442 0.64 +1.3 432,414 + amt1 Synpcc7942_0442 0.64 +3.2 432,435 - amt1 Synpcc7942_0442 0.65 -3.2 432,457 - amt1 Synpcc7942_0442 0.67 -1.5 432,469 + amt1 Synpcc7942_0442 0.68 +0.5 432,477 + amt1 Synpcc7942_0442 0.68 -2.1 432,516 - amt1 Synpcc7942_0442 0.71 -0.7 432,538 + amt1 Synpcc7942_0442 0.72 -1.1 432,609 - amt1 Synpcc7942_0442 0.77 -2.5 432,696 + amt1 Synpcc7942_0442 0.83 -0.3 432,717 + amt1 Synpcc7942_0442 0.84 -1.6 432,717 + amt1 Synpcc7942_0442 0.84 -1.9 432,717 + amt1 Synpcc7942_0442 0.84 -0.4 432,725 - amt1 Synpcc7942_0442 0.85 -1.4 432,725 - amt1 Synpcc7942_0442 0.85 -0.8 432,727 + amt1 Synpcc7942_0442 0.85 -2.1 432,738 - amt1 Synpcc7942_0442 0.86 -1.6 432,777 + amt1 Synpcc7942_0442 0.88 -2.9 432,795 - amt1 Synpcc7942_0442 0.89 -2.4 432,824 - +2.8 432,864 - -2.5 432,865 + -2.4 432,873 - -2.1 432,907 - +1.1 432,928 + -0.1 432,952 + -2.5 433,054 - +1.7 433,057 + -0.4 433,103 + +0.9 433,181 + +0.2 433,181 + -1.8 433,186 + -3.4 433,189 - -0.1 433,189 - +1.0 433,189 - +2.4 433,189 - +0.1 433,194 - +2.2 433,204 - Synpcc7942_0443 0.10 -2.1 433,238 - Synpcc7942_0443 0.13 +2.0 433,239 + Synpcc7942_0443 0.13 -1.6 433,245 - Synpcc7942_0443 0.14 -0.1 433,265 + +2.6 433,270 - Synpcc7942_0443 0.16 +0.2 433,273 - Synpcc7942_0443 0.16 +1.0 433,281 + Synpcc7942_0443 0.17 -0.1 433,288 - Synpcc7942_0443 0.17 +1.0 433,288 - Synpcc7942_0443 0.17 -0.5 433,305 - Synpcc7942_0443 0.18 -0.3 433,307 + Synpcc7942_0443 0.19 -1.1 433,309 + Synpcc7942_0443 0.19 -0.3 433,315 - Synpcc7942_0443 0.19 +0.2 433,325 - Synpcc7942_0443 0.20 +1.0 433,346 + Synpcc7942_0443 0.22 +2.7 433,373 + Synpcc7942_0443 0.24 +1.2 433,376 - Synpcc7942_0443 0.24 -0.6 433,506 + Synpcc7942_0443 0.35 -0.7 433,512 - Synpcc7942_0443 0.35 -0.6 433,516 + Synpcc7942_0443 0.35 +0.2 433,594 + Synpcc7942_0443 0.42 +1.3 433,594 + Synpcc7942_0443 0.42 +1.3 433,602 - Synpcc7942_0443 0.42 -0.6 433,602 - Synpcc7942_0443 0.42 +0.2 433,603 - Synpcc7942_0443 0.42 -0.3 433,674 + Synpcc7942_0443 0.48 +1.0 433,680 + Synpcc7942_0443 0.49 -1.4 433,750 + Synpcc7942_0443 0.54 -1.1 433,763 - Synpcc7942_0443 0.55 -1.5 433,778 - Synpcc7942_0443 0.57 -0.8 433,788 - +2.2 433,811 + Synpcc7942_0443 0.59 +2.8 433,811 + Synpcc7942_0443 0.59 +0.8 433,876 + Synpcc7942_0443 0.64 -1.0 433,882 + Synpcc7942_0443 0.65 +1.3 433,890 - Synpcc7942_0443 0.66 -0.8 433,890 - Synpcc7942_0443 0.66 +0.3 433,918 - Synpcc7942_0443 0.68 +0.0 433,920 - Synpcc7942_0443 0.68 +0.9 433,957 - Synpcc7942_0443 0.71 +3.3 434,009 - Synpcc7942_0443 0.75 -0.4 434,104 + Synpcc7942_0443 0.83 +0.9 434,112 - Synpcc7942_0443 0.83 +0.9 434,153 - Synpcc7942_0443 0.87 -0.5 434,174 + Synpcc7942_0443 0.88 +2.7 434,182 - Synpcc7942_0443 0.89 +1.3 434,182 - Synpcc7942_0443 0.89 +1.5 434,182 - Synpcc7942_0443 0.89 +0.8 434,211 + -1.7 434,217 + -0.0 434,221 + -0.9 434,226 - -1.2 434,227 + -1.7 434,236 + +0.1 434,243 - -1.8 434,244 - +0.4 434,251 - +0.6 434,262 - -1.4 434,306 + +0.8 434,312 + +1.6 434,315 + -1.4 434,343 + +1.1 434,349 + +0.1 434,361 - +1.6 434,378 + -2.3 434,383 + +2.5 434,383 + -2.3 434,391 - +0.6 434,506 - Synpcc7942_0444 0.22 -0.8 434,516 + Synpcc7942_0444 0.23 +1.4 434,516 + Synpcc7942_0444 0.23 -1.0 434,516 + Synpcc7942_0444 0.23 +2.3 434,522 - Synpcc7942_0444 0.24 +3.7 434,523 + Synpcc7942_0444 0.24 +0.5 434,524 - Synpcc7942_0444 0.24 -2.3 434,524 - Synpcc7942_0444 0.24 -1.2 434,524 - Synpcc7942_0444 0.24 -1.0 434,526 + Synpcc7942_0444 0.25 +0.3 434,526 + Synpcc7942_0444 0.25 -0.3 434,528 + Synpcc7942_0444 0.25 -0.2 434,533 + Synpcc7942_0444 0.26 -0.3 434,534 - Synpcc7942_0444 0.26 -1.1 434,541 - Synpcc7942_0444 0.27 +2.2 434,567 - Synpcc7942_0444 0.31 +2.0 434,652 + Synpcc7942_0444 0.43 +1.2 434,660 - Synpcc7942_0444 0.44 -0.6 434,692 - Synpcc7942_0444 0.49 +5.8 434,731 - Synpcc7942_0444 0.55 -0.2 434,749 + Synpcc7942_0444 0.57 +0.5 434,754 - Synpcc7942_0444 0.58 +0.4 434,789 + Synpcc7942_0444 0.63 -0.3 434,846 - Synpcc7942_0444 0.71 -1.1 434,856 - Synpcc7942_0444 0.73 -0.1 434,875 + Synpcc7942_0444 0.76 -2.2 434,898 + Synpcc7942_0444 0.79 -0.2 434,948 + Synpcc7942_0444 0.86 -2.7 435,016 + +2.3 435,016 + +0.6 435,016 + -0.9 435,018 + -0.7 435,026 - +4.7 435,043 - -0.5 435,117 - -0.8 435,308 - -0.7 435,310 + +0.1 435,310 + -0.8
Or see this region's nucleotide sequence