Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt coaE and Synpcc7942_0274 are separated by 37 nucleotides Synpcc7942_0274 and Synpcc7942_0275 overlap by 11 nucleotides Synpcc7942_0275 and glcD are separated by 11 nucleotides glcD and ligA are separated by 19 nucleotides
Synpcc7942_0273: coaE - Dephospho-CoA kinase, at 268,662 to 269,291
coaE
Synpcc7942_0274: Synpcc7942_0274 - hypothetical protein, at 269,329 to 269,667
_0274
Synpcc7942_0275: Synpcc7942_0275 - hypothetical protein, at 269,657 to 269,989
_0275
Synpcc7942_0276: glcD - glycolate oxidase subunit GlcD, at 270,001 to 271,473
glcD
Synpcc7942_0277: ligA - NAD-dependent DNA ligase LigA, at 271,493 to 273,532
ligA
Position (kb) 270
271
272 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 269.351 kb on - strand at 269.804 kb on + strand, within Synpcc7942_0275 at 270.162 kb on + strand, within glcD at 270.219 kb on + strand, within glcD at 270.273 kb on - strand, within glcD at 270.395 kb on - strand, within glcD at 270.411 kb on + strand, within glcD at 270.419 kb on - strand, within glcD at 270.431 kb on + strand, within glcD at 270.497 kb on - strand, within glcD at 270.598 kb on + strand, within glcD at 270.598 kb on + strand, within glcD at 270.701 kb on - strand, within glcD at 270.701 kb on - strand, within glcD at 270.730 kb on + strand, within glcD at 270.738 kb on - strand, within glcD at 270.753 kb on + strand, within glcD at 270.854 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.891 kb on - strand, within glcD at 270.897 kb on - strand, within glcD at 270.931 kb on - strand, within glcD at 270.941 kb on - strand, within glcD at 270.953 kb on - strand, within glcD at 270.982 kb on + strand, within glcD at 270.988 kb on - strand, within glcD at 271.032 kb on + strand, within glcD at 271.218 kb on + strand, within glcD at 271.342 kb on - strand at 271.344 kb on + strand at 271.352 kb on - strand at 271.352 kb on - strand at 271.399 kb on + strand at 271.399 kb on + strand at 271.407 kb on - strand at 271.407 kb on - strand at 271.457 kb on + strand at 271.474 kb on + strand at 271.493 kb on + strand at 271.498 kb on + strand at 271.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 269,351 - +1.5 269,804 + Synpcc7942_0275 0.44 -0.5 270,162 + glcD Synpcc7942_0276 0.11 +1.5 270,219 + glcD Synpcc7942_0276 0.15 +3.7 270,273 - glcD Synpcc7942_0276 0.18 +4.4 270,395 - glcD Synpcc7942_0276 0.27 +0.1 270,411 + glcD Synpcc7942_0276 0.28 +4.7 270,419 - glcD Synpcc7942_0276 0.28 +3.6 270,431 + glcD Synpcc7942_0276 0.29 +4.6 270,497 - glcD Synpcc7942_0276 0.34 +2.2 270,598 + glcD Synpcc7942_0276 0.41 +3.2 270,598 + glcD Synpcc7942_0276 0.41 +3.6 270,701 - glcD Synpcc7942_0276 0.48 -2.2 270,701 - glcD Synpcc7942_0276 0.48 +2.0 270,730 + glcD Synpcc7942_0276 0.49 +1.6 270,738 - glcD Synpcc7942_0276 0.50 +3.9 270,753 + glcD Synpcc7942_0276 0.51 -2.1 270,854 - glcD Synpcc7942_0276 0.58 +1.1 270,881 - glcD Synpcc7942_0276 0.60 +2.8 270,881 - glcD Synpcc7942_0276 0.60 -1.6 270,881 - glcD Synpcc7942_0276 0.60 +1.5 270,881 - glcD Synpcc7942_0276 0.60 +3.2 270,881 - glcD Synpcc7942_0276 0.60 +4.8 270,891 - glcD Synpcc7942_0276 0.60 +0.4 270,897 - glcD Synpcc7942_0276 0.61 +2.5 270,931 - glcD Synpcc7942_0276 0.63 +2.3 270,941 - glcD Synpcc7942_0276 0.64 +1.7 270,953 - glcD Synpcc7942_0276 0.65 +1.1 270,982 + glcD Synpcc7942_0276 0.67 +2.5 270,988 - glcD Synpcc7942_0276 0.67 +3.2 271,032 + glcD Synpcc7942_0276 0.70 +3.6 271,218 + glcD Synpcc7942_0276 0.83 -1.7 271,342 - -1.8 271,344 + +3.1 271,352 - +2.1 271,352 - -1.1 271,399 + +2.4 271,399 + +2.9 271,407 - +4.4 271,407 - +3.3 271,457 + +0.3 271,474 + -0.1 271,493 + +1.0 271,498 + -0.3 271,509 - +0.1
Or see this region's nucleotide sequence