Experiment: Putrescine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1496 and Pf6N2E2_1497 overlap by 4 nucleotides Pf6N2E2_1497 and Pf6N2E2_1498 overlap by 4 nucleotides Pf6N2E2_1498 and Pf6N2E2_1499 are separated by 52 nucleotides
Pf6N2E2_1496 - Aspartate aminotransferase (EC 2.6.1.1), at 1,749,482 to 1,750,693
_1496
Pf6N2E2_1497 - D-2-hydroxyglutarate dehydrogenase, at 1,750,690 to 1,752,114
_1497
Pf6N2E2_1498 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,752,111 to 1,752,962
_1498
Pf6N2E2_1499 - ABC transporter amino acid-binding protein, at 1,753,015 to 1,753,857
_1499
Position (kb)
1750
1751
1752
1753 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1749.798 kb on + strand, within Pf6N2E2_1496 at 1749.969 kb on + strand, within Pf6N2E2_1496 at 1749.969 kb on + strand, within Pf6N2E2_1496 at 1749.970 kb on - strand, within Pf6N2E2_1496 at 1750.063 kb on + strand, within Pf6N2E2_1496 at 1750.071 kb on + strand, within Pf6N2E2_1496 at 1750.500 kb on + strand, within Pf6N2E2_1496 at 1750.501 kb on - strand, within Pf6N2E2_1496 at 1750.501 kb on - strand, within Pf6N2E2_1496 at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.683 kb on + strand at 1750.684 kb on - strand at 1750.684 kb on - strand at 1750.684 kb on - strand at 1750.805 kb on - strand at 1750.880 kb on - strand, within Pf6N2E2_1497 at 1750.949 kb on + strand, within Pf6N2E2_1497 at 1750.949 kb on + strand, within Pf6N2E2_1497 at 1750.985 kb on + strand, within Pf6N2E2_1497 at 1751.085 kb on + strand, within Pf6N2E2_1497 at 1751.116 kb on - strand, within Pf6N2E2_1497 at 1751.116 kb on - strand, within Pf6N2E2_1497 at 1751.499 kb on + strand, within Pf6N2E2_1497 at 1751.499 kb on + strand, within Pf6N2E2_1497 at 1751.500 kb on - strand, within Pf6N2E2_1497 at 1751.987 kb on - strand at 1752.036 kb on - strand at 1752.146 kb on - strand at 1752.381 kb on + strand, within Pf6N2E2_1498 at 1752.381 kb on + strand, within Pf6N2E2_1498 at 1752.382 kb on - strand, within Pf6N2E2_1498 at 1752.382 kb on - strand, within Pf6N2E2_1498 at 1752.387 kb on + strand, within Pf6N2E2_1498 at 1752.388 kb on - strand, within Pf6N2E2_1498 at 1752.593 kb on - strand, within Pf6N2E2_1498 at 1752.608 kb on - strand, within Pf6N2E2_1498 at 1752.769 kb on + strand, within Pf6N2E2_1498 at 1752.770 kb on - strand, within Pf6N2E2_1498 at 1752.770 kb on - strand, within Pf6N2E2_1498 at 1752.970 kb on + strand at 1752.970 kb on + strand at 1752.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (C) remove 1,749,798 + Pf6N2E2_1496 0.26 -0.5 1,749,969 + Pf6N2E2_1496 0.40 -1.0 1,749,969 + Pf6N2E2_1496 0.40 +1.1 1,749,970 - Pf6N2E2_1496 0.40 +0.3 1,750,063 + Pf6N2E2_1496 0.48 +0.1 1,750,071 + Pf6N2E2_1496 0.49 -0.5 1,750,500 + Pf6N2E2_1496 0.84 -1.8 1,750,501 - Pf6N2E2_1496 0.84 -0.8 1,750,501 - Pf6N2E2_1496 0.84 +0.8 1,750,683 + -0.4 1,750,683 + +0.1 1,750,683 + -1.2 1,750,683 + +0.4 1,750,683 + +1.0 1,750,684 - -1.5 1,750,684 - +1.0 1,750,684 - -1.7 1,750,805 - +0.5 1,750,880 - Pf6N2E2_1497 0.13 -0.3 1,750,949 + Pf6N2E2_1497 0.18 -0.3 1,750,949 + Pf6N2E2_1497 0.18 -0.1 1,750,985 + Pf6N2E2_1497 0.21 +0.4 1,751,085 + Pf6N2E2_1497 0.28 +0.6 1,751,116 - Pf6N2E2_1497 0.30 +0.8 1,751,116 - Pf6N2E2_1497 0.30 -0.6 1,751,499 + Pf6N2E2_1497 0.57 -0.5 1,751,499 + Pf6N2E2_1497 0.57 -0.2 1,751,500 - Pf6N2E2_1497 0.57 -1.6 1,751,987 - +0.6 1,752,036 - -0.6 1,752,146 - -0.3 1,752,381 + Pf6N2E2_1498 0.32 +0.4 1,752,381 + Pf6N2E2_1498 0.32 -0.2 1,752,382 - Pf6N2E2_1498 0.32 -0.1 1,752,382 - Pf6N2E2_1498 0.32 -0.5 1,752,387 + Pf6N2E2_1498 0.32 -0.3 1,752,388 - Pf6N2E2_1498 0.33 +0.3 1,752,593 - Pf6N2E2_1498 0.57 +0.9 1,752,608 - Pf6N2E2_1498 0.58 -0.3 1,752,769 + Pf6N2E2_1498 0.77 +0.7 1,752,770 - Pf6N2E2_1498 0.77 -0.0 1,752,770 - Pf6N2E2_1498 0.77 +0.1 1,752,970 + -0.1 1,752,970 + +1.9 1,752,971 - +0.3
Or see this region's nucleotide sequence